{"id":1808,"date":"2014-03-18T01:19:59","date_gmt":"2014-03-17T18:19:59","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1808"},"modified":"2014-03-18T01:19:59","modified_gmt":"2014-03-17T18:19:59","slug":"qtls-chu-luc-lam-giam-anh-huong-xau-cua-nhiet-do-nong-den-ham-luong-amylose-cua-hat-lua-thong-qua-hieu-qua-cat-phan-tu-rna-thanh-mrna","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/qtls-chu-luc-lam-giam-anh-huong-xau-cua-nhiet-do-nong-den-ham-luong-amylose-cua-hat-lua-thong-qua-hieu-qua-cat-phan-tu-rna-thanh-mrna\/","title":{"rendered":"QTLs ch\u1ee7 l\u1ef1c l\u00e0m gi\u1ea3m \u1ea3nh h\u01b0\u1edfng x\u1ea5u c\u1ee7a nhi\u1ec7t \u0111\u1ed9 n\u00f3ng \u0111\u1ebfn h\u00e0m l\u01b0\u1ee3ng amylose c\u1ee7a h\u1ea1t l\u00faa, th\u00f4ng qua hi\u1ec7u qu\u1ea3 c\u1eaft ph\u00e2n t\u1eed RNA th\u00e0nh mRNA"},"content":{"rendered":"
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C\u00e1c nh\u00e0 khoa h\u1ecdc Trung Qu\u1ed1c \u0111\u00e3 t\u00ecm th\u1ea5y 4 QTLs c\u00f3 ch\u1ee9c n\u0103ng duy tr\u00ec h\u00e0m l\u01b0\u1ee3ng amylose c\u1ee7a g\u1ea1o khi nhi\u1ec7t \u0111\u1ed9 t\u0103ng cao b\u1eb1ng c\u00e1ch t\u0103ng c\u01b0\u1eddng ho\u1ea1t \u0111\u1ed9ng splicing m\u1ed9t c\u00e1ch c\u00f3 hi\u1ec7u qu\u1ea3 \u0111\u1ed1i v\u1edbi gen Wx.<\/span>\u0110\u0103ng ng\u00e0y 18-03-2014<\/span> trong chuy\u00ean m\u1ee5c Tin th\u1ebf gi\u1edbi<\/a><\/p>\n<\/div>\n

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H\u00e0m l\u01b0\u1ee3ng amylose \u0111\u01b0\u1ee3c \u0111i\u1ec1u khi\u1ec3n b\u1edfi\u00a0gen Wx.<\/em><\/strong>\u00a0\u0110\u00e2y l\u00e0 t\u00ednh tr\u1ea1ng ch\u1ee7 \u0111\u1ea1o quy\u1ebft \u0111\u1ecbnh ph\u1ea9m ch\u1ea5t n\u1ea5u n\u01b0\u1edbng v\u00e0 ph\u1ea9m ch\u1ea5t c\u01a1m c\u1ee7a gi\u1ed1ng l\u00faa n\u00e0o \u0111\u00f3. Trong su\u1ed1t giai \u0111o\u1ea1n v\u00e0o ch\u1eafc c\u1ee7a h\u1ea1t, nhi\u1ec7t \u0111\u1ed9 n\u00f3ng c\u1ee7a kh\u00ed quy\u1ec3n c\u00f3 th\u1ec3 l\u00e0m t\u1ed5n th\u01b0\u01a1ng ph\u1ea9m ch\u1ea5t h\u1ea1t b\u1eb1ng c\u00e1ch l\u00e0m gi\u1ea3m ho\u1eb7c m\u1ed9t c\u00e1ch c\u00f3 \u00fd ngh\u0129a h\u00e0m l\u01b0\u1ee3ng amylose trong nhi\u1ec1u gi\u1ed1ng l\u00faa. C\u00e1c t\u00e1c gi\u1ea3 ch\u1ee9ng minh v\u1ec1 m\u1eb7t di truy\u1ec1n h\u1ecdc r\u1eb1ng: s\u1ef1 th\u1ec3 hi\u1ec7n\u00a0gen Wx<\/em>\u00a0v\u00e0 h\u00e0m l\u01b0\u1ee3ng amylose \u1edf nhi\u1ec7t \u0111\u1ed9 n\u00f3ng high, c\u00f3 nhi\u1ec1u QTL \u0111\u00f3ng vai tr\u00f2 \u0111i\u1ec1u h\u00e0nh.<\/p>\n

H\u1ecd \u0111\u00e3 th\u1ef1c hi\u1ec7n m\u1ed9t k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 \u201cgenome-wide survey<\/strong>\u201d tr\u00ean m\u1ed9t qu\u1ea7n th\u1ec3 con lai\u00a0CCSLs\u00a0<\/strong>(chromosome segment substitution lines). Qu\u1ea7n th\u1ec3 n\u00e0y mang c\u00e1c \u0111o\u1ea1n ph\u00e2n th\u1ec3 c\u00f3 tr\u00ean c\u00e1c nhi\u1ec5m s\u1eafc th\u1ec3 m\u1ee5c ti\u00eau c\u1ee7a gi\u1ed1ng l\u00faa indica ch\u1ed1ng ch\u1ecbu n\u00f3ng\u00a09311<\/strong>\u00a0lai v\u1edbi gi\u1ed1ng l\u00faa japonica r\u1ea5t nh\u1ea1y c\u1ea3m v\u1edbi nhi\u1ec7t \u0111\u1ed9 n\u00f3ng \u201cNipponbare<\/strong>\u201d.<\/p>\n

B\u1ed1n QTLs \u0111\u00f3 l\u00e0QTls, qHAC4, qHAC8a, qHAC8<\/strong>b v\u00e0\u00a0qHAC10<\/strong>. Ch\u00fang c\u00f3 vai tr\u00f2 l\u00e0m gi\u1ea3m c\u00e1c \u1ea3nh h\u01b0\u1edfng b\u1ea5t l\u1ee3i \u0111\u1ebfn h\u00e0m l\u01b0\u1ee3ng amylose \u1edf \u0111i\u1ec1u ki\u1ec7n nhi\u1ec7t \u0111\u1ed9 cao. Ch\u00fang \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh n\u1eb1m tr\u00ean nhi\u1ec5m s\u1eafc th\u1ec3 s\u1ed1 4, 8, 8 v\u00e0 10, theo th\u1ee9 t\u1ef1. QTL ch\u1ee7 l\u1ef1c\u00a0qHAC8a<\/strong>, c\u00f3 gi\u00e1 tr\u1ecb LOD cao (6,196), \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng b\u1ea3n \u0111\u1ed3 v\u1eadt l\u00fd t\u1ea1i m\u1ed9t \u0111o\u1ea1n ph\u00e2n t\u1eed ng\u1eafn (small chromosome segment: ~300 kb).<\/p>\n

Qu\u1ea7n th\u1ec3 c\u00e1c d\u00f2ng con lai\u00a0CSSLs<\/strong>\u00a0mang nh\u1eefng QTL:\u00a0qHAC8a, qHAC8b<\/strong>\u00a0v\u00e0\/ho\u1eb7c\u00a0qHAC4<\/strong>\u00a0t\u1eeb gi\u1ed1ng l\u00faa\u00a09311<\/strong>\u00a0c\u00f3 hi\u1ec7u qu\u1ea3 \u201csplicing: cao \u0111\u1ed1i v\u1edbi ph\u00e2n t\u1eed\u00a0pre-mRNA<\/strong>\u00a0c\u1ee7a genWx<\/em>. Ch\u00fang \u0111\u01b0\u1ee3c xem nh\u01b0 c\u00f3 ch\u1ee9c n\u0103ng l\u00e0m \u1ed5n \u0111\u1ecbnh h\u00e0m l\u01b0\u1ee3ng amylose khi nhi\u1ec7t \u0111\u1ed9 n\u00f3ng x\u1ea3y ra. Do \u0111\u00f3, n\u1ebfu gia t\u0103ng \u0111\u01b0\u1ee3c hi\u1ec7u qu\u1ea3 th\u00fac \u0111\u1ea9y ti\u1ebfn tr\u00ecnh\u00a0pre-mRNA<\/strong>\u00a0\u0111\u1ed1i v\u1edbi gen\u00a0Wx<\/em>, \u0111\u00e2y s\u1ebd l\u00e0 m\u1ed9t c\u01a1 ch\u1ebf \u0111i\u1ec1u ti\u1ebft v\u00f4 c\u00f9ng quan tr\u1ecdng duy tr\u00ec \u0111\u01b0\u1ee3c h\u00e0m l\u01b0\u1ee3ng amylose c\u1ee7a gi\u1ed1ng l\u00faa \u1edf \u0111i\u1ec1u ki\u1ec7n nhi\u1ec7t \u0111\u1ed9 cao. H\u01a1n n\u1eefa, \u0111\u00e2y l\u00e0 k\u1ebft qu\u1ea3 \u0111\u00f3ng g\u00f3p v\u1ec1 m\u1eb7t l\u00fd thuy\u1ebft l\u00e0m c\u01a1 s\u1edf cho nh\u00e0 ch\u1ecdn gi\u1ed1ng l\u00faa ch\u1ed1ng ch\u1ecbu n\u00f3ng.<\/p>\n

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Xem chi ti\u1ebft t\u1ea1i\u00a0\u00a0http:\/\/link.springer.com\/article\/10.1007\/s00122-013-2216-4<\/a><\/p>\n

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H\u00ecnh<\/u><\/strong>.\u00a0\u00a0B\u1ea3n \u0111\u1ed3 QTL h\u00e0m l\u01b0\u1ee3ng amylose trong h\u1ea1t g\u1ea1o khi ph\u1ea3n \u1ee9ng v\u1edbi nhi\u1ec7t \u0111\u1ed9 kh\u00ed quy\u1ec3n n\u00f3ng<\/strong>(HT).<\/p>\n

H\u00ecnh A<\/strong>: B\u1ea3n \u0111\u1ed3 QTL h\u00e0m l\u01b0\u1ee3ng amylose trong \u0111i\u1ec1u ki\u1ec7n nhi\u1ec7t \u0111\u1ed9 n\u00f3ng v\u1edbi ki\u1ec3u ph\u00e2n t\u00edch :Single-QTL. Gi\u00e1 tr\u1ecb LOD c\u00f3 \u00fd ngh\u0129a t\u1ea1i 1.000 v\u1ecb tr\u00ed (1,000 permutations) (LOD = 3,79, p = 0,01); m\u0169i t\u00ean ch\u1ec9 ra c\u00e1c v\u1ecb tr\u00ed \u0111\u00e1nh d\u1ea5u.<\/p>\n

H\u00ecnh B<\/strong>: B\u1ea3n \u0111\u1ed3 v\u1eadt l\u00fd c\u1ee7a nh\u1eefng QTLs trong b\u1ed9 gen c\u00e2y l\u00faa. C\u00e1c thanh m\u00e0u x\u00e1m bi\u1ec3u hi\u1ec7n cho c\u00e1c \u201csubstituted segments\u201d t\u1eeb gi\u1ed1ng cho gen kh\u00e1ng v\u00e0 th\u00e0nh tr\u1ed1ng bi\u1ec3u hi\u1ec7n cho nh\u1eefng \u0111\u00f3ng g\u00f3p t\u1eeb nhi\u1ec5m s\u1eafc th\u1ec3 c\u1ee7a gi\u1ed1ng Nipponbare. Gi\u00e1 tr\u1ecb\u00a0DD<\/strong>\u00a0c\u1ee7a c\u00e1c d\u00f2ng CSSL (chromosome segment sub-stitution lines) \u0111\u01b0\u1ee3c xem x\u00e9t th\u00f4ng qua \u0111\u00e1nh gi\u00e1 ki\u1ec3u h\u00ecnh.<\/p>\n

Theo IAS<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

C\u00e1c nh\u00e0 khoa h\u1ecdc Trung Qu\u1ed1c \u0111\u00e3 t\u00ecm th\u1ea5y 4 QTLs c\u00f3 ch\u1ee9c n\u0103ng duy tr\u00ec h\u00e0m l\u01b0\u1ee3ng amylose c\u1ee7a g\u1ea1o khi nhi\u1ec7t \u0111\u1ed9 t\u0103ng cao b\u1eb1ng c\u00e1ch t\u0103ng c\u01b0\u1eddng ho\u1ea1t \u0111\u1ed9ng splicing m\u1ed9t c\u00e1ch c\u00f3 hi\u1ec7u qu\u1ea3 \u0111\u1ed1i v\u1edbi gen Wx.\u0110\u0103ng ng\u00e0y 18-03-2014 trong chuy\u00ean m\u1ee5c Tin th\u1ebf gi\u1edbi H\u00e0m l\u01b0\u1ee3ng amylose \u0111\u01b0\u1ee3c \u0111i\u1ec1u […]\n","protected":false},"author":1,"featured_media":1818,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/1808"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=1808"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/1808\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/1818"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=1808"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=1808"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=1808"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}