{"id":1870,"date":"2014-02-13T01:38:21","date_gmt":"2014-02-12T18:38:21","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1870"},"modified":"2014-02-13T01:38:21","modified_gmt":"2014-02-12T18:38:21","slug":"nghien-cuu-ve-gen-pit1-tren-bo","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/nghien-cuu-ve-gen-pit1-tren-bo\/","title":{"rendered":"Nghi\u00ean c\u1ee9u v\u1ec1 gen PIT1 tr\u00ean B\u00f2"},"content":{"rendered":"
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\u1ede b\u00f2 gen PIT1 c\u00f3 tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed kho\u1ea3ng 33 kDa, n\u1eb1m tr\u00ean nhi\u1ec5m s\u1eafc th\u1ec3 s\u1ed1 1 v\u00e0 n\u1eb1m \u1edf gi\u1eefa TGLA57 v\u00e0 RM95 bao g\u1ed3m 6 exon m\u00e3 h\u00f3a cho chu\u1ed7i polypeptide g\u1ed3m 291 axit amin.<\/span>\u0110\u0103ng ng\u00e0y 13-02-2014<\/span> trong chuy\u00ean m\u1ee5c Tin th\u1ebf gi\u1edbi<\/a><\/p>\n<\/div>\n

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cDNA c\u1ee7a gen PIT1 \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch tr\u00ecnh t\u1ef1 tr\u00ean nhi\u1ec1u lo\u00e0i kh\u00e1c nhau (Tatsumi v\u00e0 cs, 1992; Wong v\u00e0 cs, 1992; Yamada v\u00e0 cs, 1993).\u00a0Nhi\u1ec1u c\u00f4ng tr\u00ecnh nghi\u00ean c\u1ee9u v\u1ec1 gen tr\u00ean B\u00f2 \u0111\u00e3 c\u00f4ng b\u1ed1 c\u00f3 li\u00ean quan \u0111\u1ebfn tr\u1ecdng l\u01b0\u1ee3ng c\u01a1 th\u1ec3, t\u0103ng tr\u1ecdng trung b\u00ecnh h\u00e0ng ng\u00e0y (Renaville v\u00e0 cs, 1997a; Carrijo v\u00e0 cs,. 2008) v\u00e0 t\u00ednh tr\u1ea1ng s\u1ea3n xu\u1ea5t s\u1eefa (Renaville v\u00e0 cs, 1997b.; de Mattos v\u00e0 cs, 2004; Xue v\u00e0 cs, 2006).<\/p>\n

V\u1edbi k\u1ef9 thu\u1eadt PCR-RFLP, \u0111a h\u00ecnh\u00a0Hin<\/em>fI \u0111\u00e3 \u0111\u01b0\u1ee3c t\u00ecm th\u1ea5y \u1edf exon 6\u00a0 \u0111\u00f3 l\u00e0 s\u1ef1 thay th\u1ebf G\/A (Woollard v\u00e0 cs., 1994; Dierkes v\u00e0 cs., 1998). K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u gen PIT1 c\u1ee7a Renaville<\/span>\u00a0v\u00e0 cs (1997) cho th\u1ea5y gia s\u00fac c\u00f3 t\u1ec9 l\u1ec7 ki\u1ec3u gen BB c\u00f3 tr\u1ecdng l\u01b0\u1ee3ng c\u01a1 th\u1ec3\u00a0 l\u00fac 7 th\u00e1ng tu\u1ed5i cao h\u01a1n gia s\u00fac c\u00f3 ki\u1ec3u gen \u1edf AB v\u00e0 AA trong khi \u0111\u00f3 gia s\u00fac c\u00f3 ki\u1ec3u gen AA v\u00e0 BB \u0111\u00e3 v\u01b0\u1ee3t tr\u1ed9i so v\u1edbi gia s\u00fac c\u00f3 ki\u1ec3u gen AB \u1edf t\u00ednh tr\u1ea1ng chi\u1ec1u cao vai l\u00fac 13 th\u00e1ng, tuy nhi\u00ean kh\u00f4ng t\u00ecm th\u1ea5y m\u1ed1i quan h\u1ec7 ki\u1ec3u gen v\u1edbi t\u0103ng tr\u1ecdng trung b\u00ecnh ng\u00e0y ho\u1eb7c ti\u00eau h\u00f3a. Theo \u0111\u00f3 4 \u0111a h\u00ecnh kh\u00e1c c\u0169ng \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh n\u1eb1m trong intron 3, intron 4 v\u00e0 intron 5 (Zhao v\u00e0 cs., 2004).<\/p>\n

G\u1ea7n \u0111\u00e2y, c\u00e1c nghi\u00ean c\u1ee9u c\u0169ng \u0111\u00e3 ph\u00e1t hi\u1ec7n m\u1edbi v\u1ec1 \u0111\u1ed9t bi\u1ebfn l\u1eb7n trong exon 2 (Pan v\u00e0 cs., 2008), \u0111\u1ed9t bi\u1ebfn \u0111i\u1ec3m (SNP) l\u00e0 m\u1ed9t thay th\u1ebf G\/A t\u1ea1i v\u1ecb tr\u00ed 545 (NM_174579: c.545G) d\u1ef1a v\u00e0o ph\u01b0\u01a1ng ph\u00e1p PCR-RFLP v\u1edbi enzyme gi\u1edbi h\u1ea1n\u00a0Taq<\/em>I. K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u c\u1ee7a Chen (2011) tr\u00ean m\u1ed9t s\u1ed1 gi\u1ed1ng gi\u1ed1ng B\u00f2 Trung Qu\u1ed1c v\u00e0 gi\u1ed1ng B\u00f2 Holstein \u0111\u00e3 ph\u00e1t hi\u1ec7n c\u00e1c \u0111a h\u00ecnh trong c\u00e1c intron PIT1-5 v\u1edbi t\u1ea7n s\u1ed1 alen A \/ B \u00a0\u1edf c\u00e1c gi\u1ed1ng t\u01b0\u01a1ng \u1ee9ng nh\u01b0 sau: Nanyang (NY), Qinchuan (QC), Jiaxianhong (JXH), Xizhen (XZ), Luxi (LX) v\u00e0 qu\u1ea7n th\u1ec3 b\u00f2 Holstein (H) l\u00e0 0.444\/0.556, 0.477\/0.523, 0.538\/0.462, 0.421\/0.579, 0.523\/0.477, 0.475\/0.525.<\/p>\n

Nghi\u00ean c\u1ee9u c\u0169ng cho th\u1ea5y c\u00f3 m\u1ed1i quan h\u1ec7 gi\u1eefa ki\u1ec3u gen v\u1edbi \u00a0t\u1ed1c \u0111\u1ed9 t\u0103ng tr\u01b0\u1edfng, tr\u1ecdng l\u01b0\u1ee3ng c\u01a1 th\u1ec3, t\u0103ng tr\u1ecdng b\u00ecnh qu\u00e2n ng\u00e0y, chi\u1ec1u cao c\u01a1 th\u1ec3, chu vi ng\u1ef1c 6, 12, 18 v\u00e0 24 th\u00e1ng (P <0,01). Tr\u1ecdng l\u01b0\u1ee3ng c\u01a1 th\u1ec3 v\u00e0 k\u00edch th\u01b0\u1edbc c\u01a1 th\u1ec3 c\u0169ng cho th\u1ea5y m\u1ed9t xu h\u01b0\u1edbng c\u1ee7a alen B> alen A trong c\u00e1c nh\u00f3m tu\u1ed5i kh\u00e1c. V\u00ec v\u1eady, ki\u1ec3u gen BB c\u00f3 th\u1ec3 l\u00e0 m\u1ed9t ki\u1ec3u gen chi ph\u1ed1i v\u00e0 allele B c\u00f3 th\u1ec3 l\u00e0 m\u1ed9t allele tr\u1ed9i. Nh\u01b0 v\u1eady t\u1eeb c\u00e1c k\u1ebft qu\u1ea3 cho th\u1ea5y alen B c\u1ee7a gen PIT1 c\u00f3 \u1ea3nh h\u01b0\u1edfng t\u00edch c\u1ef1c \u0111\u1ebfn t\u00ednh tr\u1ea1ng sinh tr\u01b0\u1edfng.<\/p>\n

Theo IAS<\/span><\/em><\/strong><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u1ede b\u00f2 gen PIT1 c\u00f3 tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed kho\u1ea3ng 33 kDa, n\u1eb1m tr\u00ean nhi\u1ec5m s\u1eafc th\u1ec3 s\u1ed1 1 v\u00e0 n\u1eb1m \u1edf gi\u1eefa TGLA57 v\u00e0 RM95 bao g\u1ed3m 6 exon m\u00e3 h\u00f3a cho chu\u1ed7i polypeptide g\u1ed3m 291 axit amin.\u0110\u0103ng ng\u00e0y 13-02-2014 trong chuy\u00ean m\u1ee5c Tin th\u1ebf gi\u1edbi cDNA c\u1ee7a gen PIT1 \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch […]\n","protected":false},"author":1,"featured_media":1881,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/1870"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=1870"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/1870\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/1881"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=1870"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=1870"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=1870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}