{"id":5916,"date":"2014-10-01T04:28:20","date_gmt":"2014-09-30T21:28:20","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=770"},"modified":"2014-10-01T04:28:20","modified_gmt":"2014-09-30T21:28:20","slug":"chuyen-gien-dieu-khien-chiu-han-dreb2aca-vao-cay-bong-gossypoum-hirsutum-l-thong-qua-a-tumefaciens","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/chuyen-gien-dieu-khien-chiu-han-dreb2aca-vao-cay-bong-gossypoum-hirsutum-l-thong-qua-a-tumefaciens\/","title":{"rendered":"Chuy\u1ec3n gien \u0111i\u1ec1u khi\u1ec3n ch\u1ecbu h\u1ea1n DREB2ACA v\u00e0o c\u00e2y b\u00f4ng (Gossypoum hirsutum L.) th\u00f4ng qua A. tumefaciens"},"content":{"rendered":"
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Nghi\u00ean c\u1ee9u do\u00a0nh\u00f3m t\u00e1c gi\u1ea3 Nguy\u1ec5n Th\u1ecb Nh\u00e3, Nguy\u1ec5n Th\u1ecb T\u00e2m, Tr\u1ecbnh Th\u1ecb V\u00e2n Anh, Th\u00e1i Th\u1ecb L\u1ec7 H\u1eb1ng, Tr\u1ecbnh Minh H\u1ee3p v\u00e0 L\u00ea Tr\u1ecdng T\u00ecnh thu\u1ed9c Vi\u1ec7n Nghi\u00ean c\u1ee9u B\u00f4ng v\u00e0 Ph\u00e1t tri\u1ec3n N\u00f4ng nghi\u1ec7p Nha H\u1ed1 v\u00e0 B\u00f9i Minh Tr\u00ed thu\u1ed9c Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc N\u00f4ng L\u00e2m TP. H\u1ed3 Ch\u00ed Minh th\u1ef1c hi\u1ec7n.<\/p>\n

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Nghi\u00ean c\u1ee9u \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n nh\u1eb1m nghi\u00ean c\u1ee9u \u1ea3nh h\u01b0\u1edfng m\u1eadt \u0111\u1ed9 vi khu\u1ea9n v\u00e0 th\u1eddi gian nhi\u1ec5m Atumefaciens \u0111\u1ebfn hi\u1ec7u qu\u1ea3 chuy\u1ec3n gien m\u00e3 h\u00f3a nh\u00e2n t\u1ed1 phi\u00ean m\u00e3 li\u00ean quan \u0111\u1ebfn h\u1ea1n DREB2ACA v\u00e0o gi\u1ed1ng b\u00f4ng Coker312 (Gossypium hirsutum L.) th\u00f4ng qua A. tumefaciens ch\u1ee7ng LBA4404.<\/p>\n

K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u cho th\u1ea5y, m\u1eadt \u0111\u1ed9 v\u00e0 th\u1eddi gian nhi\u1ec5m A. tumefaciens \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u qu\u1ea3 chuy\u1ec3n gien, trong \u0111\u00f3, m\u1eadt \u0111\u1ed9 OD600\u00a00,75 k\u1ebft h\u1ee3p th\u1eddi gian l\u00e2y nhi\u1ec5m 10 ph\u00fat cho hi\u1ec7u qu\u1ea3 t\u1ed1t nh\u1ea5t, c\u00e1c ch\u1ec9 ti\u00eau v\u1ec1 t\u1ef7 l\u1ec7 m\u00f4 s\u1eb9o chuy\u1ec3n gien h\u00ecnh th\u00e0nh (34,4%), t\u1ef7 l\u1ec7 m\u00f4 s\u1eb9o s\u1ed1ng s\u00f3t sau ch\u1ecdn l\u1ecdc (26,7%) c\u0169ng nh\u01b0 t\u1ef7 l\u1ec7 m\u00f4 s\u1eb9o ph\u00e2n h\u00f3a ph\u00f4i (3,3%) \u0111\u1ec1u \u0111\u1ea1t cao nh\u1ea5t.<\/span><\/p>\n

Ti\u1ebfn h\u00e0nh 3 \u0111\u1ee3t chuy\u1ec3n gien thu \u0111\u01b0\u1ee3c 87 c\u00e2y chuy\u1ec3n gien th\u1ebf h\u1ec7 T0\u00a0kh\u00e1ng hygromyxin, trong s\u1ed1 \u0111\u00f3 c\u00f3 60 c\u00e2y mang \u0111\u1ed3ng th\u1eddi 2 gien\u00a0hpt,\u00a0<\/em>DREB2ACA v\u00e0 promoter\u00a0lip9<\/em>\u00a0v\u00e0 c\u00f3 21 c\u00e2y sinh tr\u01b0\u1edfng, ph\u00e1t tri\u1ec3n t\u1ed1t, c\u00f3 ki\u1ec3u h\u00ecnh t\u01b0\u01a1ng \u0111\u1ed3ng v\u1edbi gi\u1ed1ng g\u1ed1c v\u00e0 h\u1eefu d\u1ee5c. \u0110\u00e1nh gi\u00e1 21 d\u00f2ng chuy\u1ec3n gien th\u1ebf h\u1ec7 T1\u00a0thu \u0111\u01b0\u1ee3c 15 d\u00f2ng kh\u00e1ng hygromyxin v\u1edbi t\u1ef7 l\u1ec7 25 – 100%, mang \u0111\u1ed3ng th\u1eddi 2 gien\u00a0hpt,<\/em>DREB2ACA v\u00e0 promoter\u00a0lip9<\/em>\u00a0(12,5 -100%). \u0110\u00e1nh gi\u00e1 8 d\u00f2ng chuy\u1ec3n gien th\u1ebf h\u1ec7 T2\u00a0thu \u0111\u01b0\u1ee3c c\u1ea3 8 d\u00f2ng \u0111\u1ec1u kh\u00e1ng hygromyxin v\u1edbi t\u1ef7 l\u1ec7 50 – 100% v\u00e0 mang \u0111\u1ed3ng th\u1eddi 2 gien\u00a0hpt,\u00a0<\/em>DREB2ACA v\u00e0 promoter\u00a0lip9<\/em>.<\/span><\/p>\n

Qua k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u nh\u00f3m t\u00e1c gi\u1ea3 \u0111\u00e3 \u0111\u1ec1 ngh\u1ecb: Ti\u1ebfp t\u1ee5c \u0111\u00e1nh gi\u00e1 s\u1ef1 bi\u1ec3u hi\u1ec7n c\u1ee7a gien chuy\u1ec3n trong 8 d\u00f2ng chuy\u1ec3n gien, t\u00ednh ch\u1ecbu h\u1ea1n c\u0169ng nh\u01b0 c\u00e1c \u0111\u1eb7c t\u00ednh n\u00f4ng sinh h\u1ecdc kh\u00e1c \u0111\u1ec3 ch\u1ecdn l\u1ecdc \u0111\u01b0\u1ee3c d\u00f2ng b\u00f4ng chuy\u1ec3n gien ch\u1ecbu h\u1ea1n t\u1ed1t nh\u1ea5t.<\/span><\/p>\n

Theo TC NN & PTNT<\/span><\/strong><\/em><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Nghi\u00ean c\u1ee9u do\u00a0nh\u00f3m t\u00e1c gi\u1ea3 Nguy\u1ec5n Th\u1ecb Nh\u00e3, Nguy\u1ec5n Th\u1ecb T\u00e2m, Tr\u1ecbnh Th\u1ecb V\u00e2n Anh, Th\u00e1i Th\u1ecb L\u1ec7 H\u1eb1ng, Tr\u1ecbnh Minh H\u1ee3p v\u00e0 L\u00ea Tr\u1ecdng T\u00ecnh thu\u1ed9c Vi\u1ec7n Nghi\u00ean c\u1ee9u B\u00f4ng v\u00e0 Ph\u00e1t tri\u1ec3n N\u00f4ng nghi\u1ec7p Nha H\u1ed1 v\u00e0 B\u00f9i Minh Tr\u00ed thu\u1ed9c Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc N\u00f4ng L\u00e2m TP. H\u1ed3 Ch\u00ed Minh th\u1ef1c hi\u1ec7n.   […]\n","protected":false},"author":1,"featured_media":771,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/5916"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=5916"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/5916\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/771"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=5916"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=5916"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=5916"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}