{"id":5918,"date":"2014-08-25T04:32:19","date_gmt":"2014-08-24T21:32:19","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=777"},"modified":"2014-08-25T04:32:19","modified_gmt":"2014-08-24T21:32:19","slug":"tach-dong-tao-plasmit-mang-gen-epsps-ung-dung-cho-phat-hien-dinh-luong-dau-nanh-glycine-max-l-chuyen-gen-khang-thuoc-diet-co-glyphosate-bang-ky-thuat-real-time-pcr","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/tach-dong-tao-plasmit-mang-gen-epsps-ung-dung-cho-phat-hien-dinh-luong-dau-nanh-glycine-max-l-chuyen-gen-khang-thuoc-diet-co-glyphosate-bang-ky-thuat-real-time-pcr\/","title":{"rendered":"T\u00e1ch d\u00f2ng, t\u1ea1o Plasmit mang gen EPSPS \u1ee9ng d\u1ee5ng cho ph\u00e1t hi\u1ec7n \u0111\u1ecbnh l\u01b0\u1ee3ng \u0111\u1eadu n\u00e0nh (Glycine max L.) chuy\u1ec3n gen kh\u00e1ng thu\u1ed1c di\u1ec7t c\u1ecf Glyphosate b\u1eb1ng k\u1ef9 thu\u1eadt Real \u2013 Time PCR"},"content":{"rendered":"<div class=\"TomTat clearfix\">\n<h1><\/h1>\n<p><span id=\"ArticalDetail1_lblTeaser\" class=\"OverollContentDesc\">Nghi\u00ean c\u1ee9u t\u1eadp trung\u00a0v\u00e0o vi\u1ec7c t\u1ea1o d\u00f2ng gen EPSPS \u1edf E. coli, sau \u0111\u00f3 thu nh\u1eadn s\u1ea3n ph\u1ea9m t\u1ea1o d\u00f2ng, tinh s\u1ea1ch v\u00e0 ti\u1ebfn h\u00e0nh x\u00e2y d\u1ef1ng \u0111\u01b0\u1eddng chu\u1ea9n cho ph\u1ea3n \u1ee9ng \u0111\u1ecbnh l\u01b0\u1ee3ng, ph\u00e1t hi\u1ec7n gen EPSPS \u0111\u01b0\u1ee3c chuy\u1ec3n tr\u00ean \u0111\u1eadu n\u00e0nh b\u1eb1ng Real &#8211; time PCR.<\/span><\/p>\n<\/div>\n<div class=\"NoiDungBai\">\n<p>Nghi\u00ean c\u1ee9u do nh\u00f3m t\u00e1c gi\u1ea3 B\u00f9i C\u00e1ch Tuy\u1ebfn (T\u1ed5ng C\u1ee5c M\u00f4i tr\u01b0\u1eddng, B\u1ed9 T\u00e0i nguy\u00ean v\u00e0 M\u00f4i tr\u01b0\u1eddng), \u0110inh Th\u00e0nh Ph\u01b0\u1edbc (B\u1ed9 m\u00f4n C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc \u2013 Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc N\u00f4ng L\u00e2m TP. H\u1ed3 Ch\u00ed Minh), Ph\u1ea1m Th\u1ecb Anh Th\u01b0 (Trung t\u00e2m Nghi\u00ean c\u1ee9u Khoa h\u1ecdc Qu\u1ed1c gia Ph\u00e1p (CNRS) v\u00e0 Hu\u1ef3nh V\u0103n Bi\u1ebft \u2013 Vi\u1ec7n Nghi\u00ean c\u1ee9u C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc v\u00e0 M\u00f4i tr\u01b0\u1eddng, Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc N\u00f4ng L\u00e2m TP. H\u1ed3 Ch\u00ed Minh) th\u1ef1c hi\u1ec7n nh\u1eb1m m\u1ee5c ti\u00eau l\u00e0 t\u1ea1o \u0111\u01b0\u1ee3c c\u00e1c m\u1eabu chu\u1ea9n mang gien EPSPS (5-enolpyruvylsgikimate-3-phosphate synthase) c\u00f3 \u0111\u1ed9 tinh s\u1ea1ch cao \u0111\u1ec3 x\u00e2y d\u1ef1ng \u0111\u01b0\u1ee3c \u0111\u01b0\u1eddng chu\u1ea9n cho \u0111\u1ecbnh l\u01b0\u1ee3ng gien EPSPS kh\u00e1ng thu\u1ed1c di\u1ec7t c\u1ecf v\u1edbi chi ph\u00ed th\u1ea5p v\u00e0 ti\u1ebft ki\u1ec7m, nh\u1eb1m ph\u00e1t hi\u1ec7n \u0111\u1ecbnh l\u01b0\u1ee3ng c\u00e2y \u0111\u1eadu n\u00e0nh v\u00e0 s\u1ea3n ph\u1ea9m t\u1eeb \u0111\u1eadu n\u00e0nh chuy\u1ec3n gien n\u00e0y.<\/p>\n<p class=\"Bodytext20\">Qua nghi\u00ean c\u1ee9u nh\u00f3m t\u00e1c gi\u1ea3 \u0111\u00e3 t\u1ea1o \u0111\u01b0\u1ee3c E. coli \u00a0kh\u1ea3 n\u1ea1p c\u00f3 kh\u1ea3 n\u0103ng nh\u1eadn plasmit; t\u1ea1o d\u00f2ng th\u00e0nh c\u00f4ng gien EPSPS; x\u00e2y d\u1ef1ng \u0111\u01b0\u1ee3c \u0111\u01b0\u1eddng chu\u1ea9n cho \u0111\u1ecbnh l\u01b0\u1ee3ng chuy\u1ec3n gien EPSPS kh\u00e1ng thu\u1ed1c di\u1ec7t c\u1ecf tr\u00ean \u0111\u1eadu n\u00e0nh b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p Real &#8211; time PCR c\u00f3 ph\u01b0\u01a1ng tr\u00ecnh h\u1ed3i quy = -3,0533x + 54,4187 v\u00f3i \u0111\u1ed9 tin c\u1eady cao (R2\u00a0= 0,9992).<\/p>\n<p class=\"Bodytext20\"><span class=\"noi-dung-chi-tiet\">K\u1ebft qu\u1ea3 th\u1eed nghi\u1ec7m b\u1eb1ng k\u1ef9 thu\u1eadt Real &#8211; time PCR v\u1edbi \u0111\u01b0\u1eddng chu\u1ea9n x\u00e1c l\u1eadp \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n m\u1eabu ADN \u0111\u1eadu n\u00e0nh s\u1ed1 1 v\u00e0 s\u1ed1 2 c\u00f3 mang gien EPSPS. S\u1ed1 l\u01b0\u1ee3ng gien chuy\u1ec3n t\u01b0\u01a1ng \u1ee9ng t\u00ean m\u1eabu ADN \u0111\u1eadu n\u00e0nh s\u1ed1 1 l\u00e0 211,34 x 106\u00a0b\u1ea3n sao v\u00e0 m\u1eabu ADN \u0111\u1eadu n\u00e0nh s\u1ed1 2 l\u00e0 1,21 x 109\u00a0b\u1ea3n sao.<\/span><\/p>\n<p class=\"BodyText6\"><span class=\"noi-dung-chi-tiet\">Qua k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u, nh\u00f3m t\u00e1c gi\u1ea3 \u0111\u00e3 \u0111\u1ec1 ngh\u1ecb n\u00ean th\u1ef1c hi\u1ec7n nghi\u00ean c\u1ee9u t\u1ea1o d\u00f2ng nhi\u1ec1u tr\u00ecnh t\u1ef1 gien \u0111\u01b0\u1ee3c chuy\u1ec3n v\u00e0o c\u00e2y tr\u1ed3ng, t\u1ea1o d\u00f2ng c\u00e1c tr\u00ecnh t\u1ef1 promoter (ch\u1ea5t ho\u1ea1t h\u00f3a), terminater (\u0111i\u1ec3m k\u1ebft th\u00fac) v\u00e0 nhi\u1ec1u tr\u00ecnh t\u1ef1 kh\u00e1c trong c\u1ea5u tr\u00fac gien chuy\u1ec3n \u0111\u1ec3 t\u1ea1o \u0111\u01b0\u1ee3c nhi\u1ec1u m\u1eabu chu\u1ea9n v\u00e0 \u0111\u01b0\u1eddng chu\u1ea9n cho ki\u1ec3m tra \u0111\u1ecbnh l\u01b0\u1ee3ng chuy\u1ec3n gien nh\u1eb1m t\u0103ng m\u1ee9c \u0111\u1ed9 chuy\u00ean bi\u1ec7t trong ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng c\u01a1 quan bi\u1ebfn \u0111\u1ed5i di truy\u1ec1n (GMOs).<\/span><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Nghi\u00ean c\u1ee9u t\u1eadp trung\u00a0v\u00e0o vi\u1ec7c t\u1ea1o d\u00f2ng gen EPSPS \u1edf E. coli, sau \u0111\u00f3 thu nh\u1eadn s\u1ea3n ph\u1ea9m t\u1ea1o d\u00f2ng, tinh s\u1ea1ch v\u00e0 ti\u1ebfn h\u00e0nh x\u00e2y d\u1ef1ng \u0111\u01b0\u1eddng chu\u1ea9n cho ph\u1ea3n \u1ee9ng \u0111\u1ecbnh l\u01b0\u1ee3ng, ph\u00e1t hi\u1ec7n gen EPSPS \u0111\u01b0\u1ee3c chuy\u1ec3n tr\u00ean \u0111\u1eadu n\u00e0nh b\u1eb1ng Real &#8211; time PCR. Nghi\u00ean c\u1ee9u do nh\u00f3m t\u00e1c gi\u1ea3 B\u00f9i [&#8230;]\n","protected":false},"author":1,"featured_media":5921,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/5918"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=5918"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/5918\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/5921"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=5918"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=5918"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=5918"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}