{"id":5962,"date":"2014-02-12T06:57:32","date_gmt":"2014-02-11T23:57:32","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=941"},"modified":"2014-02-12T06:57:32","modified_gmt":"2014-02-11T23:57:32","slug":"thiet-lap-quy-trinh-tai-sinh-cay-in-vitro-va-khao-sat-kha-nang-chuyen-nap-gen-vao-cay-ca-chua-lycopersicon-esculentum-thong-qua-agrobacterium-tumefaciens","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/thiet-lap-quy-trinh-tai-sinh-cay-in-vitro-va-khao-sat-kha-nang-chuyen-nap-gen-vao-cay-ca-chua-lycopersicon-esculentum-thong-qua-agrobacterium-tumefaciens\/","title":{"rendered":"Thi\u1ebft l\u1eadp quy tr\u00ecnh t\u00e1i sinh c\u00e2y in vitro v\u00e0 kh\u1ea3o s\u00e1t kh\u1ea3 n\u0103ng chuy\u1ec3n n\u1ea1p gen v\u00e0o c\u00e2y c\u00e0 chua (Lycopersicon esculentum) th\u00f4ng qua Agrobacterium Tumefaciens"},"content":{"rendered":"<div class=\"TomTat clearfix\">\n<p>C\u00e2y c\u00e0 chua c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb ch\u00e2u M\u1ef9 v\u00e0 ph\u1ed5 bi\u1ebfn ra to\u00e0n th\u1ebf gi\u1edbi t\u1eeb sau th\u1ebf k\u1ef7 16, v\u1edbi kh\u1ea3 n\u0103ng th\u00edch \u1ee9ng cao, c\u00e0 chua g\u1ea7n nh\u01b0 \u0111\u01b0\u1ee3c tr\u1ed3ng \u1edf m\u1ecdi n\u01a1i tr\u00ean th\u1ebf gi\u1edbi v\u00e0 mang l\u1ea1i gi\u00e1 tr\u1ecb kinh t\u1ebf cao.<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<div class=\"NoiDungBai\">\n<p>Nghi\u00ean c\u1ee9u do nh\u00f3m c\u00e1c t\u00e1c gi\u1ea3 B\u00f9i C\u00e1ch Tuy\u1ebfn (B\u1ed9 T\u00e0i nguy\u00ean v\u00e0 M\u00f4i tr\u01b0\u1eddng), Nguy\u1ec5n Xu\u00e2n Tu\u1ea5n v\u00e0 c\u00e1c c\u1ed9ng s\u1ef1 (Vi\u1ec7n C\u00f4ng ngh\u1ec7 sinh h\u1ecdc v\u00e0 M\u00f4i tr\u01b0\u1eddng, \u0110H N\u00f4ng L\u00e2m TP. HCM) c\u00f9ng th\u1ef1c hi\u1ec7n, v\u1edbi m\u1ee5c \u0111\u00edch nh\u1eb1m t\u1ea1o ti\u1ec1n \u0111\u1ec1 cho c\u00e1c nghi\u00ean c\u1ee9u ti\u1ebfp sau \u0111\u1ec3 c\u1ea3i thi\u1ec7n n\u0103ng su\u1ea5t, t\u0103ng s\u1ea3n l\u01b0\u1ee3ng v\u00e0 t\u00ednh ch\u1ed1ng ch\u1ecbu tr\u00ean c\u00e2y c\u00e0 chua.<\/p>\n<p>Nghi\u00ean c\u1ee9u \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh tr\u00ean hai gi\u1ed1ng c\u00e0 chua (F1Fn576 v\u00e0 Nicola F1NT538) \u0111\u01b0\u1ee3c cung c\u1ea5p b\u1edfi C\u00f4ng ty Gi\u1ed1ng Trang N\u00f4ng. K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u cho th\u1ea5y: ch\u1ed3i t\u00e1i sinh t\u1eeb callus (th\u1ec3 chai) c\u1ee7a \u0111o\u1ea1n th\u00e2n m\u1ea7m v\u00e0 l\u00e1 \u0111\u01b0\u1ee3c \u0111i\u1ec1u khi\u1ec3n b\u1edfi s\u1ef1 k\u1ebft h\u1ee3p gi\u1eefa n\u1ed3ng \u0111\u1ed9 kinetin v\u00e0 gi\u1ed1ng. Quy tr\u00ecnh t\u1ed1t nh\u1ea5t \u0111\u1ec3 t\u00e1i sinh ch\u1ed3i \u0111\u01b0\u1ee3c ghi nh\u1eadn tr\u00ean gi\u1ed1ng c\u00e0 chua F1Fn576 v\u00e0 m\u00f4i tr\u01b0\u1eddng MS b\u1ed5 sung 1 mg\/l kinetin + 1 mg\/l IAA. V\u1edbi m\u1eabu l\u00e1 t\u1ec9 l\u1ec7 m\u1eabu t\u1ea1o ch\u1ed3i \u0111\u1ea1t 78,8% v\u00e0 4 ch\u1ed3i\/m\u1eabu, c\u00f2n tr\u00ean m\u1eabu th\u00e2n m\u1ea7m t\u1ec9 l\u1ec7 n\u00e0y \u0111\u1ea1t 37,77% v\u00e0 0,24 ch\u1ed3i\/m\u1eabu (ghi nh\u1eadn sau 4 tu\u1ea7n nu\u00f4i c\u1ea5y). M\u00f4i tr\u01b0\u1eddng hi\u1ec7u qu\u1ea3 cho vi\u1ec7c nh\u00e2n ch\u1ed3i \u1edf \u0111o\u1ea1n th\u00e2n m\u1ea7m sau 4 tu\u1ea7n quan s\u00e1t l\u00e0 MS + 1 mg\/l BA (2,2 ch\u1ed3i\/c\u1ee5m ch\u1ed3i, \u0111\u1ea1t chi\u1ec1u d\u00e0i ch\u1ed3i 3,13 cm).\u00a0C\u00e1c ch\u1ed3i kh\u1ecfe \u0111\u01b0\u1ee3c \u0111em t\u1ea1o r\u1ec5 tr\u00ean m\u00f4i tr\u01b0\u1eddng MS \u0111\u1ea1t 27 r\u1ec5\/m\u1eabu v\u00e0 chi\u1ec1u d\u00e0i r\u1ec5 \u0111\u1ea1t 3,49 cm.<\/p>\n<p>Ch\u1ee7ng vi khu\u1ea9n Agrobacterium tumefaciens GV3580 mang plasmit pGII0229 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 chuy\u1ec3n v\u00e0o c\u00e2y c\u00e0 chua. B\u01b0\u1edbc \u0111\u1ea7u ghi nh\u1eadn k\u1ebft qu\u1ea3 bi\u1ec3u hi\u1ec7n c\u1ee7a gien gusA \u0111\u01b0\u1ee3c chuy\u1ec3n v\u00e0o h\u1ec7 gien c\u1ee7a c\u00e2y, hi\u1ec7u qu\u1ea3 chuy\u1ec3n gien \u0111\u1ea1t 100% \u1edf l\u00e1 v\u00e0 71% \u1edf \u0111o\u1ea1n th\u00e2n m\u1ea7m khi \u1ee7 trong d\u1ecbch khu\u1ea9n v\u1edbi th\u1eddi gian l\u00e0 15 ph\u00fat, n\u1ed3ng \u0111\u1ed9 axetosyringon l\u00e0 100mM.<\/p>\n<p>K\u1ebft qu\u1ea3 in vitro tr\u00ean m\u00f4i tr\u01b0\u1eddng ch\u1ecdn l\u1ecdc c\u00e1c m\u1eabu c\u00e0 chua l\u00e2y nhi\u1ec5m vi khu\u1ea9n \u0111\u00e3 thu \u0111\u01b0\u1ee3c 2 d\u00f2ng c\u00e0 chua in vitro c\u00f3 kh\u1ea3 n\u0103ng s\u1ed1ng \u0111\u01b0\u1ee3c trong m\u00f4i tr\u01b0\u1eddng ch\u1ecdn l\u1ecdc v\u1edbi PPT. V\u00e0 k\u1ebft qu\u1ea3 PCR t\u1eeb m\u1eabu l\u00e1 2 d\u00f2ng n\u00e0y \u0111\u00e3 x\u00e1c nh\u1eadn s\u1ef1 c\u00f3 m\u1eb7t c\u1ee7a gien bar trong b\u1ed9 gien c\u1ee7a c\u00e2y c\u00e0 chua.<\/p>\n<p><em><strong><span class=\"noi-dung-chi-tiet\">Theo Ph\u00f2ng Qu\u1ea3n l\u00fd ngu\u1ed3n gen v\u00e0 An to\u00e0n sinh h\u1ecdc,<\/span><\/strong><\/em><\/p>\n<p><em><strong><span class=\"noi-dung-chi-tiet\">C\u1ee5c B\u1ea3o t\u1ed3n \u0111a d\u1ea1ng sinh h\u1ecdc<\/span><\/strong><\/em><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>C\u00e2y c\u00e0 chua c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb ch\u00e2u M\u1ef9 v\u00e0 ph\u1ed5 bi\u1ebfn ra to\u00e0n th\u1ebf gi\u1edbi t\u1eeb sau th\u1ebf k\u1ef7 16, v\u1edbi kh\u1ea3 n\u0103ng th\u00edch \u1ee9ng cao, c\u00e0 chua g\u1ea7n nh\u01b0 \u0111\u01b0\u1ee3c tr\u1ed3ng \u1edf m\u1ecdi n\u01a1i tr\u00ean th\u1ebf gi\u1edbi v\u00e0 mang l\u1ea1i gi\u00e1 tr\u1ecb kinh t\u1ebf cao. &nbsp; Nghi\u00ean c\u1ee9u do nh\u00f3m c\u00e1c t\u00e1c gi\u1ea3 [&#8230;]\n","protected":false},"author":1,"featured_media":948,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/5962"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=5962"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/5962\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/948"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=5962"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=5962"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=5962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}