{"id":6000,"date":"2013-06-03T07:38:25","date_gmt":"2013-06-03T00:38:25","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1131"},"modified":"2013-06-03T07:38:25","modified_gmt":"2013-06-03T00:38:25","slug":"ung-dung-ky-thuat-dna-gop-phan-vao-viec-phan-loai-hinh-thai-mot-so-loai-tre-viet-nam","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/ung-dung-ky-thuat-dna-gop-phan-vao-viec-phan-loai-hinh-thai-mot-so-loai-tre-viet-nam\/","title":{"rendered":"\u1ee8ng d\u1ee5ng k\u1ef9 thu\u1eadt DNA g\u00f3p ph\u1ea7n v\u00e0o vi\u1ec7c ph\u00e2n lo\u1ea1i h\u00ecnh th\u00e1i m\u1ed9t s\u1ed1 lo\u00e0i tre Vi\u1ec7t Nam"},"content":{"rendered":"
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Tre ph\u00e2n b\u1ed1 r\u1ed9ng kh\u1eafp m\u1ecdi n\u01a1i \u1edf Vi\u1ec7t Nam. \u0110\u00e2y l\u00e0 ngu\u1ed3n t\u00e0i nguy\u00ean c\u00f3 gi\u00e1 tr\u1ecb trong nhi\u1ec1u l\u0129nh v\u1ef1c nh\u01b0 c\u00f4ng nghi\u1ec7p, gia d\u1ee5ng, c\u1ea3nh quan, th\u1ef1c ph\u1ea9m.<\/p>\n

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Cu\u1ed1i th\u1ebf k\u1ef7 XIX (1890), nh\u00e0 th\u1ef1c v\u1eadt h\u1ecdc ng\u01b0\u1eddi Ph\u00e1p Balansa l\u1ea7n \u0111\u1ea7u ti\u00ean ph\u00e2n lo\u1ea1i tre Vi\u1ec7t Nam: c\u00f3 5 chi v\u00e0 7 lo\u00e0i, trong \u0111\u00f3 c\u00f3 2 lo\u00e0i m\u1edbi. Theo th\u1eddi gian s\u1ed1 l\u01b0\u1ee3ng taxon ng\u00e0y m\u1ed9t t\u0103ng, theo Nguy\u1ec5n Ho\u00e0ng Ngh\u0129a (2006) chi tre tr\u00fac \u1edf Vi\u1ec7t Nam c\u00f3 25 chi v\u00e0 216 lo\u00e0i, trong \u0111\u00f3 chi tre (Bambusa<\/em>\u00a0Schreb.) c\u00f3 67 lo\u00e0i, th\u00ec c\u00f3 t\u1edbi 37 lo\u00e0i ch\u01b0a \u0111\u1ecbnh lo\u1ea1i \u0111\u01b0\u1ee3c t\u00ean lo\u00e0i (d\u1ea1ng sp. v\u00e0 aff.). B\u00ean c\u1ea1nh \u0111\u00f3, L\u00ea Vi\u1ebft L\u00e2m (2008) c\u0169ng \u0111\u00e3 ph\u00e1t hi\u1ec7n th\u00eam 4 lo\u00e0i m\u1edbi, trong \u0111\u00f3 2 lo\u00e0i ch\u01b0a x\u00e1c \u0111\u1ecbnh t\u00ean.<\/p>\n

Hi\u1ec7n nay, vi\u1ec7c ph\u00e2n lo\u1ea1i v\u00e0 \u0111\u1ecbnh lo\u1ea1i t\u00ean lo\u00e0i cho tre v\u1eabn ch\u1ee7 y\u1ebfu d\u1ef1a tr\u00ean ph\u01b0\u01a1ng ph\u00e1p h\u00ecnh th\u00e1i. Tuy nhi\u00ean ph\u01b0\u01a1ng ph\u00e1p \u0111\u00f3 \u0111\u00f2i h\u1ecfi m\u1eabu v\u1eadt ph\u1ea3i c\u00f3 \u0111\u1ea7y \u0111\u1ee7 c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m ph\u00e2n lo\u1ea1i, \u0111\u1eb7c bi\u1ec7t l\u00e0 c\u01a1 quan sinh s\u1ea3n, m\u00e0 \u0111\u1ed1i v\u1edbi tre th\u00ec c\u00e1c m\u1eabu v\u1eadt ch\u1ec9 c\u00f3 c\u01a1 quan dinh d\u01b0\u1ee1ng, h\u1ea7u h\u1ebft kh\u00f4ng c\u00f3 \u0111\u1eb7c \u0111i\u1ec3m v\u1ec1 c\u01a1 quan sinh s\u1ea3n (hoa, qu\u1ea3) v\u00ec chu k\u1ef3 ra hoa t\u1edbi v\u00e0i ch\u1ee5c n\u0103m. H\u01a1n n\u1eefa trong m\u1ed9t s\u1ed1 tr\u01b0\u1eddng h\u1ee3p, ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n lo\u1ea1i b\u1eb1ng h\u00ecnh th\u00e1i kh\u00f3 th\u1ef1c hi\u1ec7n, ho\u1eb7c d\u1ec5 nh\u1ea7m l\u1eabn do m\u1eabu mang \u0111\u1eb7c \u0111i\u1ec3m trung gian ho\u1eb7c \u0111\u1ed3ng h\u00ecnh. Kh\u00f3 kh\u0103n n\u00e0y kh\u00f4ng ch\u1ec9 c\u00f3 \u1edf Vi\u1ec7t Nam m\u00e0 ngay c\u1ea3 tr\u00ean th\u1ebf gi\u1edbi. V\u00ec v\u1eady, vi\u1ec7c \u0111\u1ecbnh lo\u1ea1i t\u00ean lo\u00e0i \u1edf chi tre v\u1eabn c\u00f2n r\u1ea5t nan gi\u1ea3i, c\u1ea7n c\u00f3 s\u1ef1 h\u1ed7 tr\u1ee3 c\u1ee7a k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch DNA.<\/p>\n\n\n\n
\"\"<\/td>\n\"\"<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Trong khu\u00f4n kh\u1ed5 c\u1ee7a \u0111\u1ec1 t\u00e0i\u00a0\u201cG\u00f3p ph\u1ea7n x\u00e1c \u0111\u1ecbnh c\u00e1c lo\u00e0i thu\u1ed9c chi tre (Bambusa Schreb.) \u1edf Vi\u1ec7t Nam b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p DNA h\u1ed7 tr\u1ee3 ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n lo\u1ea1i h\u00ecnh th\u00e1i truy\u1ec1n th\u1ed1ng”<\/em>\u00a0m\u00e3 s\u1ed1: VAST.\u0110L.02\/11-12, do PGS.TS. \u0110inh Th\u1ecb Ph\u00f2ng – B\u1ea3o t\u00e0ng Thi\u00ean nhi\u00ean Vi\u1ec7t Nam l\u00e0m ch\u1ee7 nhi\u1ec7m th\u1ef1c hi\u1ec7n trong hai n\u0103m 2011 v\u00e0 2012, c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u \u0111\u00e3 \u0111i th\u1ef1c \u0111\u1ecba t\u1ea1i 42 \u0111i\u1ec3m thu\u1ed9c n\u0103m v\u00f9ng l\u00e0 T\u00e2y B\u1eafc B\u1ed9, \u0110\u00f4ng B\u1eafc B\u1ed9, \u0110\u1ed3ng b\u1eb1ng S\u00f4ng H\u1ed3ng, Tr\u01b0\u1eddng S\u01a1n B\u1eafc v\u00e0 T\u00e2y Nam B\u1ed9. T\u1ed5ng s\u1ed1 \u0111\u00e3 thu \u0111\u01b0\u1ee3c 139 m\u1eabu c\u1ee7a 14 lo\u00e0i tre \u0111\u1ec3 nghi\u00ean c\u1ee9u v\u1ecb tr\u00ed ph\u00e2n lo\u1ea1i tr\u00ean c\u01a1 s\u1edf ph\u00e2n t\u00edch tr\u00ecnh t\u1ef1 5 v\u00f9ng gen (01 v\u00f9ng gen nh\u00e2n v\u00e0 04 v\u00f9ng gen l\u1ee5c l\u1ea1p). \u0110\u1ec1 t\u00e0i \u0111\u00e3 thu \u0111\u01b0\u1ee3c c\u00e1c k\u1ebft qu\u1ea3 nh\u01b0 sau:<\/p>\n

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  • M\u01b0\u1eddi b\u1ed1n lo\u00e0i tre nghi\u00ean c\u1ee9u g\u1ed3m c\u00f3 t\u00e1m lo\u00e0i c\u1ea7n x\u00e1c \u0111\u1ecbnh t\u00ean khoa h\u1ecdc (Bambusa<\/em>\u00a0sp.) l\u00e0 B\u1ea1c m\u00e0y, M\u1ea1y c\u01b0\u1ee3p, M\u1ea1y kh\u00f4, M\u00e9t ba v\u00ec, Tre kh\u00f4ng gai t\u00e2n an, Tre l\u1ee5c b\u00ecnh, Tre tr\u00e3i long an v\u00e0 Tre \u0111\u00f4ng kh\u00ea; ba lo\u00e0i ch\u1ec9nh l\u00fd t\u00ean chi l\u00e0 D\u00f9ng c\u1ea7u hai (B.<\/em>(Lingnania<\/em>) sp.), D\u00f9ng ph\u1ea5n (B.<\/em>\u00a0(Lingnania) chungii<\/em>) v\u00e0 L\u00f9ng thanh ho\u00e1 (B.<\/em>\u00a0(Lingnania) longissima<\/em>) v\u00e0 ba lo\u00e0i c\u1ea7n x\u00e1c \u0111\u1ecbnh l\u1ea1i t\u00ean khoa h\u1ecdc do bi\u1ebfn \u0111\u1ed5i h\u00ecnh th\u00e1i theo sinh th\u00e1i l\u00e0 Tre b\u1ee5ng ph\u1eadt (B.<\/em>vulgaris<\/em>), Tre v\u00e0ng s\u1ecdc (B. vulgaris<\/em>) v\u00e0 Tre \u0111\u00f9i g\u00e0 (B. ventricosa<\/em>).<\/li>\n
  • Th\u00f4ng qua k\u1ebft qu\u1ea3 ph\u00e2n t\u00edch v\u00e0 so s\u00e1nh m\u1ee9c \u0111\u1ed9 t\u01b0\u01a1ng \u0111\u1ed3ng tr\u00ecnh t\u1ef1 nucleotide c\u1ee7a b\u1ed1n v\u00f9ng gen PIF,\u00a0psbA-trnH, matK<\/em>v\u00e0\u00a0trnL-trnF<\/em>\u00a0c\u1ee7a ba lo\u00e0i tre c\u1ea7n ch\u1ec9nh l\u00fd t\u00ean chi v\u1edbi c\u00e1c tr\u00ecnh t\u1ef1 tr\u00ean ng\u00e2n h\u00e0ng Genbank cho ph\u00e9p nh\u1eadn \u0111\u1ecbnh ba lo\u00e0i D\u00f9ng c\u1ea7u hai, D\u00f9ng ph\u1ea5n v\u00e0 L\u00f9ng thanh h\u00f3a \u0111\u1ec1u thu\u1ed9c chi\u00a0Bambusa<\/em>.<\/li>\n<\/ul>\n\n\n\n\n
    \"\"<\/td>\n<\/tr>\n
    M\u1ed1i quan h\u1ec7 di truy\u1ec1n \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng b\u1eb1ng ph\u1ea7n m\u1ec1m MEGA 4.0 (ph\u01b0\u01a1ng ph\u00e1p Neighbor-Joining) gi\u1eefa 03 lo\u00e0i D\u00f9ng c\u1ea7u hai (K3001), D\u00f9ng ph\u1ea5n (K3002) v\u00e0 L\u00f9ng thanh ho\u00e1 (K3003) v\u1edbi c\u00e1c lo\u00e0i Bambusa tr\u00ean ng\u00e2n h\u00e0ng Genbank ph\u00e2n t\u00edch v\u1edbi b\u1ed1n v\u00f9ng gen PIF, psbA-trnH, matK v\u00e0 trnL-trnF<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
      \n
    • K\u1ebft qu\u1ea3 ph\u00e2n t\u00edch so s\u00e1nh m\u1ee9c \u0111\u1ed9 t\u01b0\u01a1ng \u0111\u1ed3ng tr\u00ecnh t\u1ef1 nucleotide c\u1ee7a b\u1ed1n v\u00f9ng gen PIF,\u00a0psbA-trnH, matK<\/em>\u00a0v\u00e0\u00a0trnL-trnF<\/em>\u00a0\u0111\u00e3 ch\u1ec9 ra Tre b\u1ee5ng ph\u1eadt v\u00e0 Tre v\u00e0ng s\u1ecdc thu\u1ed9c c\u00f9ng lo\u00e0i\u00a0B. vulgaris<\/em>; Tre \u0111\u00f9i g\u00e0 ch\u1ec9 l\u00e0 t\u00ean \u0111\u1ed3ng ngh\u0129a c\u1ee7a H\u00f3p nh\u1ecf (B. tuldoides<\/em>) v\u00e0 ch\u1ec9 l\u00e0 gi\u1ed1ng tr\u1ed3ng (cv.).<\/li>\n<\/ul>\n\n\n\n\n
      \"\"<\/td>\n<\/tr>\n
      M\u1ed1i quan h\u1ec7 di truy\u1ec1n \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng b\u1eb1ng ph\u1ea7n m\u1ec1m MEGA 4.0 (ph\u01b0\u01a1ng ph\u00e1p Neighbor-Joining) gi\u1eefa lo\u00e0i Tre \u0111\u00f9i g\u00e0 (K3006) v\u1edbi lo\u00e0i B. tuldoides tr\u00ean ng\u00e2n h\u00e0ng Genbank ph\u00e2n t\u00edch v\u1edbi v\u00f9ng gen trnL-trnF, psbA-trnH v\u00e0 matK ((tr\u00ecnh t\u1ef1 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 so s\u00e1nh c\u00f3 m\u00e3 hi\u1ec7u trong ng\u00e2n h\u00e0ng GenBank: v\u00f9ng gen trnL-trnF: B. tuldoides (HM448967); v\u00f9ng gen psbA-trnH: B. tuldoides (GU063083); v\u00f9ng gen: B. tuldoides (HM448937)<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
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      • B\u01b0\u1edbc \u0111\u1ea7u \u0111\u00e3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c lo\u00e0i Tre kh\u00f4ng gai t\u00e2n an c\u00f3 th\u1ec3 l\u00e0 lo\u00e0i tre m\u1edbi c\u1ee7a Vi\u1ec7t Nam tr\u00ean c\u01a1 s\u1edf x\u00e1c \u0111\u1ecbnh tr\u00ecnh t\u1ef1 n\u0103m v\u00f9ng gen (PIF,\u00a0psbA-trnH, matK, trnL-trnF<\/em>\u00a0v\u00e0\u00a0rpoC2<\/em>).<\/li>\n
      • So s\u00e1nh 19 \u0111\u1eb7c \u0111i\u1ec3m h\u00ecnh th\u00e1i v\u00e0 5 \u0111\u1eb7c \u0111i\u1ec3m ph\u00e2n t\u1eed cho th\u1ea5y lo\u00e0i M\u00e9t ba v\u00ec c\u00f3 nhi\u1ec1u \u0111\u1eb7c \u0111i\u1ec3m gi\u1ed1ng v\u1edbi lo\u00e0i M\u1ea1y c\u01b0\u1ee3p.<\/li>\n<\/ul>\n

        Th\u00f4ng qua c\u00e1c k\u1ebft qu\u1ea3 nh\u1eadn \u0111\u01b0\u1ee3c, c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u \u0111\u1ec1 ngh\u1ecb th\u1ed1ng nh\u1ea5t vi\u1ebft t\u00ean chi\u00a0Bambusa<\/em>\u00a0cho ba lo\u00e0i D\u00f9ng c\u1ea7u hai, D\u00f9ng ph\u1ea5n v\u00e0 L\u00f9ng thanh h\u00f3a (b\u1ecf\u00a0Lingnania<\/em>) v\u00e0 th\u1ed1ng nh\u1ea5t t\u00ean khoa h\u1ecdc cho hai lo\u00e0i Tre b\u1ee5ng ph\u1eadt v\u00e0 Tre v\u00e0ng s\u1ecdc l\u00e0Bambusa vulgaris<\/em>; v\u00e0 Tre \u0111\u00f9i g\u00e0 l\u00e0 t\u00ean \u0111\u1ed3ng ngh\u0129a c\u1ee7a H\u00f3p nh\u1ecf v\u00e0 c\u00f3 t\u00ean khoa h\u1ecdc\u00a0Bambusa tuldoides<\/em>.<\/p>\n

        \u0110\u00e2y l\u00e0 nghi\u00ean c\u1ee9u \u0111\u1ea7u ti\u00ean \u1edf Vi\u1ec7t Nam x\u00e1c \u0111\u1ecbnh c\u00e1c lo\u00e0i thu\u1ed9c chi tre (Bambusa<\/em>\u00a0Schreb.) b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p DNA g\u00f3p ph\u1ea7n h\u1ed7 tr\u1ee3 ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n lo\u1ea1i h\u00ecnh th\u00e1i truy\u1ec1n th\u1ed1ng. C\u00e1c k\u1ebft qu\u1ea3 nh\u1eadn \u0111\u01b0\u1ee3c ban \u0111\u1ea7u cho th\u1ea5y, \u0111\u1ec3 c\u00f3 th\u1ec3 \u1ee9ng d\u1ee5ng hi\u1ec7u qu\u1ea3 k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch ADN h\u1ed7 tr\u1ee3 cho ph\u00e2n lo\u1ea1i h\u00ecnh th\u00e1i \u1edf tre n\u00f3i ri\u00eang v\u00e0 c\u00e1c lo\u00e0i th\u1ef1c v\u1eadt kh\u00e1c n\u00f3i chung, c\u1ea7n ph\u1ea3i c\u00f3 s\u1ef1 k\u1ebft h\u1ee3p r\u1ea5t ch\u1eb7t ch\u1ebd gi\u1eefa c\u00e1c nh\u00e0 ph\u00e2n lo\u1ea1i h\u00ecnh th\u00e1i v\u00e0 sinh h\u1ecdc ph\u00e2n t\u1eed.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

        Tre ph\u00e2n b\u1ed1 r\u1ed9ng kh\u1eafp m\u1ecdi n\u01a1i \u1edf Vi\u1ec7t Nam. \u0110\u00e2y l\u00e0 ngu\u1ed3n t\u00e0i nguy\u00ean c\u00f3 gi\u00e1 tr\u1ecb trong nhi\u1ec1u l\u0129nh v\u1ef1c nh\u01b0 c\u00f4ng nghi\u1ec7p, gia d\u1ee5ng, c\u1ea3nh quan, th\u1ef1c ph\u1ea9m.   Cu\u1ed1i th\u1ebf k\u1ef7 XIX (1890), nh\u00e0 th\u1ef1c v\u1eadt h\u1ecdc ng\u01b0\u1eddi Ph\u00e1p Balansa l\u1ea7n \u0111\u1ea7u ti\u00ean ph\u00e2n lo\u1ea1i tre Vi\u1ec7t Nam: c\u00f3 5 chi […]\n","protected":false},"author":1,"featured_media":6894,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6000"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6000"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6000\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6894"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6000"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6000"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6000"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}