{"id":6641,"date":"2014-03-12T01:28:36","date_gmt":"2014-03-11T18:28:36","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1837"},"modified":"2014-03-12T01:28:36","modified_gmt":"2014-03-11T18:28:36","slug":"bo-gen-cay-san","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/bo-gen-cay-san\/","title":{"rendered":"B\u1ed9 gen c\u00e2y s\u1eafn"},"content":{"rendered":"
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R\u1ec5 c\u1ee7 s\u1eafn l\u00e0 ngu\u1ed3n l\u01b0\u01a1ng th\u1ef1c c\u1ea7n thi\u1ebft cho g\u1ea7n m\u1ed9t t\u1ef7 ng\u01b0\u1eddi tr\u00ean th\u1ebf gi\u1edbi v\u00e0 \u0111\u01b0\u1ee3c xem nh\u01b0 ngu\u1ed3n n\u0103ng l\u01b0\u1ee3ng sinh h\u1ecdc trong t\u01b0\u01a1ng lai.<\/span>\u0110\u0103ng ng\u00e0y 12-03-2014<\/span> trong chuy\u00ean m\u1ee5c Tin th\u1ebf gi\u1edbi<\/a><\/p>\n<\/div>\n

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Ng\u01b0\u1eddi ta \u0111\u00e3 t\u1eadp trung nghi\u00ean c\u1ee9u b\u1ed9 gen c\u00e2y s\u1eafn v\u1edbi hai m\u1ee5c ti\u00eau nh\u01b0 v\u1eady. B\u00ean c\u1ea1nh \u0111\u00f3, vi\u1ec7c hi\u1ec3u bi\u1ebft b\u1ed9 gen s\u00e2y s\u1eafn (cassava genome) gi\u00fap ng\u01b0\u1eddi ta qu\u1ea3n l\u00fd t\u1ed1t s\u00e2u b\u1ec7nh h\u1ea1i ch\u00ednh trong ng\u00e0nh tr\u1ed3ng s\u1eafn; t\u1ea1o ra gi\u1ed1ng s\u1eafn ch\u1ed1ng ch\u1ecbu \u0111\u01b0\u1ee3c nh\u1eefng b\u1ea5t l\u1ee3i cho bi\u1ebfn \u0111\u1ed5i kh\u00ed h\u1eadu.<\/p>\n

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Ng\u01b0\u1eddi ta th\u1ef1c hi\u1ec7n m\u1ed9t d\u1ef1 \u00e1n c\u00f3 t\u00ean l\u00e0 \u201c454-based whole genome shotgun sequence\u201d.<\/strong>\u00a0K\u1ebft qu\u1ea3 n\u00e0y \u0111\u00e3 bi\u1ebft \u0111\u01b0\u1ee3c 69% t\u1ed5ng l\u00edch th\u01b0\u1edbc genome theo d\u1ef1 \u0111o\u00e1n v\u00e0 96% kh\u00f4ng gian ch\u1ee9a c\u00e1c gen m\u00e3 h\u00f3a c\u00e1c protein \u0111\u01b0\u1ee3c bi\u1ebft. C\u00f3 30.666 gen \u0111\u01b0\u1ee3c ghi nh\u1eadn v\u00e0 3.485 d\u1ea1ng h\u00ecnh nh\u1eefng \u201calternate splice<\/strong>\u201d \u0111\u01b0\u1ee3c s\u1ef1 h\u1ed7 tr\u1ee3 c\u1ee7a ch\u1ec9 th\u1ecb ph\u00e2n t\u1eed EST (v\u1edbi 1,4 Mb ESTs).<\/p>\n

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Ng\u01b0\u1eddi ta \u0111\u00e3 \u00e1p d\u1ee5ng th\u00e0nh c\u00f4ng k\u1ef9 thu\u1eadt \u201cs\u1eed d\u1ee5ng SSR, EST v\u00e0 SNP tr\u00ean c\u1edf s\u1edf b\u1ea3n \u0111\u1ed3 di truy\u1ec1n\u201d \u0111\u1ec3 nghi\u00ean c\u1ee9u b\u1ed9 gen c\u00e2y s\u1eafn. Nh\u1edd k\u1ebft qu\u1ea3 n\u00e0y, ng\u01b0\u1eddi ta c\u00f3 \u0111\u01b0\u1ee3c m\u1ed9t b\u1ea3n \u0111\u1ed3 li\u00ean k\u1ebft c\u00f3 m\u1ee9c \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao (high-density linkage map). N\u00f3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n t\u1eeb t\u1ed5 h\u1ee3p lai gi\u1eefa hai gi\u1ed1ng s\u1eafn c\u00f3 m\u1ee9c \u0111\u1ed9 \u0111a d\u1ea1ng di truy\u1ec1n kh\u00e1c bi\u1ec7t nhau: gi\u1ed1ng s\u1eafn nhi\u1ec5m b\u1ec7nh\u00a0brown streak<\/strong>\u00a0(s\u1ecdc n\u00e2u); v\u00e0 gi\u1ed1ng kh\u00e1ng b\u1ec7nh s\u1ecdc n\u00e2u.<\/p>\n

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Ph\u01b0\u01a1ng ph\u00e1p m\u1edbi c\u00f3 t\u00ean g\u1ecdi l\u00e0\u00a0GBS<\/strong>\u00a0\u0111\u01b0\u1ee3c vi\u1ebft t\u1eaft t\u1eeb ch\u1eef \u201cgenotyping-by-sequencing\u201d (\u0111\u00e1nh gi\u00e1 ki\u1ec3u gen b\u1eb1ng gi\u1ea3i tr\u00ecnh t\u1ef1 gen) \u0111\u00e3 v\u00e0 \u0111ang \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n nh\u1eb1m li\u1ec7t k\u00ea SNPs (catalog) gi\u1eefa qu\u1ea7n th\u1ec3 l\u1eadp b\u1ea3n \u0111\u1ed3 v\u1edbi c\u00e1c gi\u1ed1ng s\u1eafn c\u1ee7a Ch\u00e2u Phi, \u0111ang \u0111\u01b0\u1ee3c n\u00f4ng d\u00e2n \u1ee9 chu\u1ed9ng. Nh\u1eefng ngu\u1ed3n k\u1ebft qu\u1ea3 nh\u01b0 v\u1eady \u0111ang th\u00fac \u0111\u1ea9y m\u1ed9t c\u00e1ch thu\u1eadn l\u1ee3i ch\u01b0\u01a1ng tr\u00ecnh ch\u1ecdn t\u1ea1o gi\u1ed1ng s\u1eafn b\u1eb1ng ch\u1ec9 th\u1ecb ph\u00e2n t\u1eed (MAS), cho ph\u00e9p ch\u00fang ta c\u1ea3i ti\u1ebfn t\u00ednh ch\u1ed1ng ch\u1ecbu b\u1ec7nh s\u1eafn v\u00e0 c\u1ea3i ti\u1ebfn h\u00e0m l\u01b0\u1ee3ng dinh d\u01b0\u1ee1ng, gi\u00fap ch\u00fang ta hi\u1ec3u c\u1eb7n k\u1ebd c\u01a1 s\u1edf di truy\u1ec1n c\u1ee7a t\u00ednh kh\u00e1ng b\u1ec7nh.<\/p>\n

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Ngu\u1ed3n:\u00a0<\/strong>Simon Prochnik v\u00e0 ctv. 2012. The Cassava Genome: Current Progress, Future Directions. Tropical Plant Biol. (2012) 5:88.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

R\u1ec5 c\u1ee7 s\u1eafn l\u00e0 ngu\u1ed3n l\u01b0\u01a1ng th\u1ef1c c\u1ea7n thi\u1ebft cho g\u1ea7n m\u1ed9t t\u1ef7 ng\u01b0\u1eddi tr\u00ean th\u1ebf gi\u1edbi v\u00e0 \u0111\u01b0\u1ee3c xem nh\u01b0 ngu\u1ed3n n\u0103ng l\u01b0\u1ee3ng sinh h\u1ecdc trong t\u01b0\u01a1ng lai.\u0110\u0103ng ng\u00e0y 12-03-2014 trong chuy\u00ean m\u1ee5c Tin th\u1ebf gi\u1edbi Ng\u01b0\u1eddi ta \u0111\u00e3 t\u1eadp trung nghi\u00ean c\u1ee9u b\u1ed9 gen c\u00e2y s\u1eafn v\u1edbi hai m\u1ee5c ti\u00eau nh\u01b0 v\u1eady. B\u00ean […]\n","protected":false},"author":1,"featured_media":6385,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6641"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6641"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6641\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6385"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6641"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6641"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6641"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}