{"id":6679,"date":"2015-05-02T08:54:52","date_gmt":"2015-05-02T01:54:52","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1440"},"modified":"2015-05-02T08:54:52","modified_gmt":"2015-05-02T01:54:52","slug":"giong-lua-bien-doi-gen-bieu-hien-suy-giam-do-bac-bung","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/giong-lua-bien-doi-gen-bieu-hien-suy-giam-do-bac-bung\/","title":{"rendered":"Gi\u1ed1ng l\u00faa bi\u1ebfn \u0111\u1ed5i gen bi\u1ec3u hi\u1ec7n suy gi\u1ea3m \u0111\u1ed9 b\u1ea1c b\u1ee5ng"},"content":{"rendered":"
Vi\u1ec7c cung c\u1ea5p kh\u00f4ng \u0111\u1ee7 l\u01b0\u1ee3ng \u201cphotosynthates\u201d (s\u1ea3n ph\u1ea9m quang h\u1ee3p c\u1ee7a h\u1ea1t g\u1ea1o \u0111\u01b0\u1ee3c xem nh\u01b0 l\u00e0 m\u1ed9t trong nh\u1eefng nguy\u00ean nh\u00e2n g\u00e2y ra b\u1ea1c b\u1ee5ng (chalkiness), l\u00e0m cho ph\u1ea9m ch\u1ea5t h\u1ea1t suy gi\u1ea3m.\u00a0<\/span><\/div>\n
\n

T\u1ea1i Trung Qu\u1ed1c, c\u00e1c nh\u00e0 khoa h\u1ecdc thu\u1ed9c \u0110H Zhaoqing, \u0111\u1ee9ng \u0111\u1ea7u l\u00e0 Yonghai Liu \u0111\u00e3 nghi\u00ean c\u1ee9u\u00a0enzyme L-galactono-1, 4-lactone dehydrogenase<\/strong>\u00a0(L-GalLDH, EC1.3.2.3<\/strong>), \u0111\u00f3ng vai tr\u00f2 x\u00fac t\u00e1c \u1edf giai \u0111o\u1ea1n cu\u1ed1i c\u1ee7a sinh t\u1ed5ng h\u1ee3pascorbic acid (Asc)<\/strong>\u00a0v\u00e0 \u1ea3nh h\u01b0\u1edfng c\u1ee7a n\u00f3 \u0111\u1ed1i v\u1edbi \u0111\u1ed9 b\u1ea1c b\u1ee5ng.<\/p>\n

H\u1ecd th\u1ea5y r\u1eb1ng s\u1eed bi\u1ec3u hi\u1ec7n m\u1ea1nh m\u1ebd\u00a0L-GalLDH<\/strong>\u00a0trong c\u00e2y l\u00faa transgenic,\u00a0GO-2<\/strong>, ch\u1ee9a h\u00e0m l\u01b0\u1ee3ng\u00a0Asc<\/strong>\u00a0trong l\u00e1 l\u00faa cao h\u01a1n c\u00e2y l\u00faa b\u00ecnh th\u01b0\u1eddng; l\u00e0m gi\u1ea3m \u0111\u1ed9 b\u1ea1c b\u1ee5ng \u0111\u00e1ng k\u1ec3. Ph\u00e2n t\u00edch s\u00e2u h\u01a1n cho th\u1ea5y khi l\u00e0m t\u0103ng m\u1ee9c \u0111\u1ed9\u00a0Asc<\/strong>\u00a0s\u1ebd l\u00e0m t\u1ed5ng h\u1ee3p raribulose-1,5-bisphosphate (RuBP)<\/strong>\u00a0cao h\u01a1n, m\u1ee9c \u0111\u1ed9 protein\u00a0carboxylase\/oxygenase (Rubisco)<\/strong>\u00a0trong\u00a0GO-2<\/strong>cao h\u01a1n, ng\u1ea7n \u1ea5y l\u00e0m t\u0103ng m\u1ee9c \u0111\u1ed9 quang h\u1ee3p c\u1ee7a c\u00e2y l\u00faa.<\/p>\n

Th\u00fac \u0111\u1ea9y s\u1ef1 t\u0103ng h\u00e0m l\u01b0\u1ee3ng Asc s\u1ebd \u1ea3n h\u01b0\u1edfng \u0111\u1ebfn b\u1ea1c b\u1ee5ng c\u1ee7a h\u1ea1t l\u00faa chuy\u1ec3n gen GO-2 th\u00f4ng qua ch\u1ee9c n\u0103ng quang h\u1ee3p trong l\u00e1 l\u00faa \u1edf giai \u0111o\u1ea1n ph\u00e1t tri\u1ec3n ch\u1ee7 y\u1ebfu li\u00ean quan \u0111\u1ebfn s\u1ef1 v\u00e0o ch\u1eafc c\u1ee7a h\u1ea1t.<\/p>\n

Xem\u00a0Science Direct<\/a>.<\/p>\n

 <\/p>\n

\"\"<\/p>\n

H\u00ecnh\u00a01.\u00a0\u0110\u1ed9 b\u1ea1c b\u1ee5ng c\u1ee7a h\u1ea1t g\u1ea1o v\u00e0 h\u00ecnh th\u00e1i h\u1ecdc c\u1ee7a tinh b\u1ed9t so s\u00e1nh gi\u1eefa gi\u1ed1ng l\u00faa bi\u1ebfn \u0111\u1ed5i gen GO-2 v\u00e0 gi\u1ed1ng nguy\u00ean th\u1ee7y (WT).<\/em><\/p>\n

(A) \u0110\u1ed9 b\u1ea1c b\u1ee5ng c\u1ee7a h\u1ea1t g\u1ea1o GO-2 v\u00e0 WT.<\/p>\n

(B) H\u1ea1t g\u1ea1o ch\u00e0 b\u00f3ng c\u1ee7a l\u00faa WT (tr\u00e1i) v\u00e0 GO-2 (ph\u1ea3i).<\/p>\n

(C) H\u00ecnh \u1ea3nh \u201cscanning electron microscopy\u201d\u00a0 nh\u1eefng ph\u1ea7n n\u1ed5i c\u1ee7a gi\u1ed1ng WT (tr\u00e1i) v\u00e0 GO-2 (ph\u1ea3i). Gi\u00e1 tr\u1ecb m\u1ed9t g\u1ea1ch l\u00e0 10\u00a0\u03bcm.<\/p>\n

S\u1ed1 li\u1ec7u trong h\u00ecnh (A) l\u00e0 gi\u00e1 tr\u1ecb trung b\u00ecnh \u00b1\u00a0SE c\u1ee7a 50 l\u1ea7n nh\u1eafc l\u1ea1i, LSD 0,05.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Vi\u1ec7c cung c\u1ea5p kh\u00f4ng \u0111\u1ee7 l\u01b0\u1ee3ng \u201cphotosynthates\u201d (s\u1ea3n ph\u1ea9m quang h\u1ee3p c\u1ee7a h\u1ea1t g\u1ea1o \u0111\u01b0\u1ee3c xem nh\u01b0 l\u00e0 m\u1ed9t trong nh\u1eefng nguy\u00ean nh\u00e2n g\u00e2y ra b\u1ea1c b\u1ee5ng (chalkiness), l\u00e0m cho ph\u1ea9m ch\u1ea5t h\u1ea1t suy gi\u1ea3m.\u00a0 T\u1ea1i Trung Qu\u1ed1c, c\u00e1c nh\u00e0 khoa h\u1ecdc thu\u1ed9c \u0110H Zhaoqing, \u0111\u1ee9ng \u0111\u1ea7u l\u00e0 Yonghai Liu \u0111\u00e3 nghi\u00ean c\u1ee9u\u00a0enzyme L-galactono-1, 4-lactone […]\n","protected":false},"author":1,"featured_media":6085,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6679"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6679"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6679\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6085"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6679"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6679"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6679"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}