{"id":6687,"date":"2014-10-01T09:38:33","date_gmt":"2014-10-01T02:38:33","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1614"},"modified":"2014-10-01T09:38:33","modified_gmt":"2014-10-01T02:38:33","slug":"su-dung-snp-va-haplotypes-de-xac-dinh-vung-gia-dinh-cua-gen-cua-hat-dau-nanh","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/su-dung-snp-va-haplotypes-de-xac-dinh-vung-gia-dinh-cua-gen-cua-hat-dau-nanh\/","title":{"rendered":"S\u1eed d\u1ee5ng SNP v\u00e0 haplotypes \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh v\u00f9ng gi\u1ea3 \u0111\u1ecbnh c\u1ee7a gen c\u1ee7a h\u1ea1t \u0111\u1eadu n\u00e0nh"},"content":{"rendered":"
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S\u1eed d\u1ee5ng\u00a0SNP (single nucleotide polymorphisms) v\u00e0 haplotypes \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh v\u00f9ng gi\u1ea3 \u0111\u1ecbnh c\u1ee7a gen m\u00e3 h\u00f3a h\u00e0m l\u01b0\u1ee3ng protein (PC) v\u00e0 protein h\u00f2a tan trong n\u01b0\u1edbc (SPC) c\u1ee7a h\u1ea1t \u0111\u1eadu n\u00e0nh<\/span><\/p>\n<\/div>\n

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C\u00f3 b\u1ed1n loci m\u00e3 h\u00f3a protein \u201cSPC-specific\u201d(h\u00e0m l\u01b0\u1ee3ng protein tan trong n\u01b0\u1edbc \u0111\u1eb7c bi\u1ec7t) \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh, gi\u1ea3i th\u00edch \u0111\u01b0\u1ee3c 8,5\u201315,1\u00a0% bi\u1ebfn thi\u1ebfn ki\u1ec3u h\u00ecnh, v\u00e0 l\u00fd gi\u1ea3i t\u1ea1i sao gi\u1ed1ng \u0111\u1eadu n\u00e0nh c\u00f3 h\u00e0m l\u01b0\u1ee3ng PC cao th\u00ec SPC th\u1ea5p.<\/p>\n

H\u00e0m l<\/strong>\u01b0\u1ee3<\/strong>ng protein tan trong n<\/strong>\u01b0\u1edb<\/strong>c<\/strong>\u00a0(SPC<\/strong>: water-soluble protein content) l\u00e0 m\u1ed9t y\u1ebfu t\u1ed1 v\u00f4 c\u00f9ng c\u1ea7n thi\u1ebft cho c\u1ea3 hai m\u1ee5c \u0111\u00edch: ph\u1ea9m ch\u1ea5t th\u1ee9c \u0103n v\u00e0 s\u1ea3n l\u01b0\u1ee3ng c\u1ee7a protein \u0111\u1eadu n\u00e0nh \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh. Tuy nhi\u00ean, c\u00f3 r\u1ea5t \u00edt s\u1ed1 li\u1ec7u v\u1ec1 b\u1ea3n ch\u1ea5t di truy\u1ec1n v\u00e0 c\u01a1 ch\u1ebf bi\u1ebfn d\u01b0\u1ee1ng l\u00e0m sao gia t\u0103ng \u0111\u01b0\u1ee3c\u00a0SPC<\/strong>. Trong th\u00ed nghi\u1ec7m n\u00e0y, ng\u01b0\u1eddi ta s\u1eed d\u1ee5ng t\u1eadp \u0111o\u00e0n \u0111\u1eadu n\u00e0nh g\u1ed1m c\u00f3 192 m\u1eabu gi\u1ed1ng c\u00f3 ngu\u1ed3n g\u1ed1c \u0111\u1ecba l\u00fd kh\u00e1 r\u1ed9ng \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh v\u00f9ng n\u00e0o trong genome c\u00e2y \u0111\u1eadu n\u00e0nh li\u00ean quan \u0111\u1ebfn h\u00e0m l\u01b0\u1ee3ng protein (PC<\/strong>: protein content) v\u00e0 h\u00e0m l\u01b0\u1ee3ng protein h\u00f2a tan trong n\u01b0\u1edbc (SPC) th\u00f4ng qua c\u00e1ch ti\u1ebfp c\u1eadn v\u1edbi ph\u01b0\u01a1ng ph\u00e1p \u201cassociation mapping<\/strong>\u201d, v\u1edbi 1.536 SNP makers v\u00e0 232 haplotypes. Bi\u1ebfn thi\u00ean di truy\u1ec1n c\u1ee7a PC v\u00e0 SPC r\u1ea5t l\u1edbn th\u00f4ng qua k\u1ebft qu\u1ea3 \u201cdiverse panel\u201d. K\u1ebft qu\u1ea3 ph\u00e2n t\u00edch \u201cassociation mapping\u201d v\u1edbi ba ph\u01b0\u01a1ng ph\u00e1p th\u00f4ng d\u1ee5ng nh\u1eb1m gi\u1ea3m thi\u1ec3u t\u1ed1i \u0111a t\u00e1c \u0111\u1ed9ng c\u1ee7a nh\u1eefng k\u1ebft h\u1ee3p mang t\u00ednh ch\u1ea5t sai l\u1ec7ch (false-positive associations).<\/p>\n

Nh\u01b0 v\u1eady, c\u00f3 4\/8\u00a0SNPs\u00a0v\u00e0 3\/6 haplotypes k\u1ebft h\u1ee3p m\u1ed9t c\u00e1ch \u00fd ngh\u0129a v\u1edbi h\u00e0m l\u01b0\u1ee3ng protein PC\/ h\u00e0m l\u01b0\u1ee3ng SPC trong hai ho\u1eb7c nhi\u1ec1u h\u01a1n m\u00f4i tr\u01b0\u1eddng tr\u00ean c\u01a1 s\u1edf xem x\u00e9t m\u00f4 ph\u1ecfng h\u1ed7n h\u1ee3p (mixed model). M\u1ed9t ch\u1ec9 th\u1ecb SNP li\u00ean k\u1ebft c\u00f3 \u00fd ngh\u0129a v\u1edbi\u00a0PC, BARC-021267-04016,<\/strong>\u0111\u1ecbnh v\u1ecb \u1edf qu\u00e3ng\u00a00.28\u00a0cM\u00a0<\/strong>v\u1edbi gen m\u00e3 h\u00f3a glycin,\u00a0G7<\/em><\/strong>, v\u00e0 \u0111\u01b0\u1ee3c t\u00ecm th\u1ea5y ch\u00fang bi\u1ec3u hi\u1ec7n qua t\u1ea5t c\u1ea3 b\u1ed1n m\u00f4i tr\u01b0\u1eddng nghi\u00ean c\u1ee9u. B\u1ed1n loci ch\u1ee7 l\u1ef1c li\u00ean quan \u0111\u1ebfn\u00a0SPC-specific<\/strong>, \u0111\u00f3 l\u00e0\u00a0BARC-029149-06088, BARC-018023-02499, BARC-041663-08059<\/strong>\u00a0v\u00e0\u00a0haplotype 15 (hp15)<\/strong>, \u0111\u01b0\u1ee3c ng\u01b0\u1eddi ta x\u00e1c \u0111\u1ecbnh tr\u00ean nhi\u1ec5m s\u1eafc th\u1ec3 5 v\u00e0 8. Ch\u00fang gi\u1ea3i th\u00edch \u0111\u01b0\u1ee3c 8.5\u201315.1\u00a0% bi\u1ebfn thi\u00ean ki\u1ec3u h\u00ecnh. H\u01a1n n\u1eefa, gen m\u00e3 h\u00f3a\u00a0glutelin type-B 2-like<\/strong>\u00a0c\u0169ng \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh tr\u00ean nhi\u1ec5m s\u1eafc th\u1ec3 8 v\u00e0 c\u00f3 kh\u1ea3 n\u0103ng li\u00ean quan \u0111\u1ebfn t\u00ednh ch\u1ea5t d\u1ec5 h\u00f2a tan c\u1ee7a protein \u0111\u1eadu n\u00e0nh.<\/p>\n

Nh\u1eefng markers nh\u01b0 v\u1eady \u0111\u1ecbnh v\u1ecb tr\u00ean QTL \u0111\u00e3 \u0111\u01b0\u1ee3c ng\u01b0\u1eddi ta b\u00e1o c\u00e1o tr\u01b0\u1edbc \u0111\u00e2y, ch\u00fang t\u00e1i kh\u1eb3ng \u0111\u1ecbnh l\u1ea1i nh\u1eefng k\u1ebft qu\u1ea3 tr\u01b0\u1edbc \u0111\u00f3 v\u00e0 tr\u1edf th\u00e0nh nh\u1eefng markers m\u1ee5c ti\u00eau \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh c\u00e1c gen c\u00f3 li\u00ean quan \u0111\u1ebfn protein. Nh\u1eefng\u00a0SNPs\u00a0m\u1edbi n\u00e0y v\u00e0 nh\u1eefng haplotypes \u1ea5y v\u00f4 c\u00f9ng quan tr\u1ecdng ph\u1ee5c v\u1ee5 cho nghi\u00ean c\u1ee9u s\u00e2u h\u01a1n b\u1ea3n ch\u1ea5t di truy\u1ec1n h\u1ecdc c\u1ee7a PC v\u00e0 SPC. Th\u00f4ng qua vi\u1ec7c so s\u00e1nh t\u01b0\u01a1ng quan v\u00e0 loci di truy\u1ec1n gi\u1eefa PC v\u00e0 SPC, ng\u01b0\u1eddi ta cung c\u1ea5p cho c\u00e1c nh\u00e0 khoa h\u1ecdc c\u00e1ch nh\u1eadn \u0111\u1ecbnh m\u1edbi v\u1ec1 gi\u1ed1ng \u0111\u1eadu n\u00e0nh t\u1ea1i sao khi h\u00e0m l\u01b0\u1ee3ng PC cao th\u00ec SPC l\u1ea1i th\u1ea5p.<\/p>\n

 <\/p>\n

Ngu\u1ed3n:\u00a0Theoretical and Applied Genetics<\/em><\/strong>\u00a0September 2014, Volume 127,\u00a0Issue 9, pp 1905-1915<\/p>\n

http:\/\/link.springer.com\/article\/10.1007\/s00122-014-2347-2<\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

S\u1eed d\u1ee5ng\u00a0SNP (single nucleotide polymorphisms) v\u00e0 haplotypes \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh v\u00f9ng gi\u1ea3 \u0111\u1ecbnh c\u1ee7a gen m\u00e3 h\u00f3a h\u00e0m l\u01b0\u1ee3ng protein (PC) v\u00e0 protein h\u00f2a tan trong n\u01b0\u1edbc (SPC) c\u1ee7a h\u1ea1t \u0111\u1eadu n\u00e0nh C\u00f3 b\u1ed1n loci m\u00e3 h\u00f3a protein \u201cSPC-specific\u201d(h\u00e0m l\u01b0\u1ee3ng protein tan trong n\u01b0\u1edbc \u0111\u1eb7c bi\u1ec7t) \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh, gi\u1ea3i th\u00edch \u0111\u01b0\u1ee3c 8,5\u201315,1\u00a0% bi\u1ebfn […]\n","protected":false},"author":1,"featured_media":6107,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6687"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6687"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6687\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6107"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6687"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6687"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}