{"id":6712,"date":"2015-07-05T08:31:53","date_gmt":"2015-07-05T01:31:53","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1334"},"modified":"2015-07-05T08:31:53","modified_gmt":"2015-07-05T01:31:53","slug":"genome-cua-giong-khoai-lang-co-phan-tu-agrobacterium-t-dnas-bieu-hien-cac-gen-dich-mot-vi-du-chuyen-gen-thanh-cong-tren-giong-cay-luong-thuc","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/genome-cua-giong-khoai-lang-co-phan-tu-agrobacterium-t-dnas-bieu-hien-cac-gen-dich-mot-vi-du-chuyen-gen-thanh-cong-tren-giong-cay-luong-thuc\/","title":{"rendered":"Genome c\u1ee7a gi\u1ed1ng khoai lang c\u00f3 ph\u00e2n t\u1eed Agrobacterium T-dnas bi\u1ec3u hi\u1ec7n c\u00e1c gen \u0111\u00edch: m\u1ed9t v\u00ed d\u1ee5 chuy\u1ec3n gen th\u00e0nh c\u00f4ng tr\u00ean gi\u1ed1ng c\u00e2y l\u01b0\u01a1ng th\u1ef1c"},"content":{"rendered":"
T\u00e1c gi\u1ea3 th\u00f4ng b\u00e1o k\u1ebft qu\u1ea3 quan s\u00e1t \u0111\u00e1ng ch\u00fa \u00fd v\u1ec1 291 m\u1eabu gi\u1ed1ng khoai lang kh\u1ea3o nghi\u1ec7m, t\u1ea5t c\u1ea3 c\u00f3 m\u1ed9t ho\u1eb7c nhi\u1ec1u tr\u00ecnh t\u1ef1 c\u1ee7a transfer DNA (T-DNA). Nh\u1eefng tr\u00ecnh t\u1ef1 n\u00e0y, bi\u1ec3u hi\u1ec7n trong m\u1ed9t d\u00f2ng khoai lang v\u00f4 t\u00ednh (gi\u1ed1ng khoai lang \u201cHuachano\u201d).<\/span><\/div>\n
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Gi\u1ed1ng n\u00e0y \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch chi ti\u1ebft cho th\u1ea5y s\u1ef1 l\u00e2y nhi\u1ec5m c\u1ee7a vi khu\u1ea9n Agrobacterium x\u1ea3y ra trong nh\u1eefng th\u1eddi \u0111i\u1ec3m c\u00f3 t\u00ednh ch\u1ea5t ti\u1ebfn h\u00f3a. M\u1ed9t trong nh\u1eefng ph\u00e2n t\u1eed T-DNAs t\u1ea1m th\u1eddi xu\u1ea5t hi\u1ec7n trong t\u1ea5t c\u1ea3 c\u00e1c d\u00f2ng v\u00f4 t\u00ednh \u00a0c\u1ee7a gi\u1ed1ng khoai lang tr\u1ed3ng tr\u1ecdt, nh\u01b0ng kh\u00f4ng c\u00f3 quan h\u1ec7 v\u1edbi c\u00e1c lo\u00e0i hoang d\u1ea1i g\u1ea7n g\u1ee7i, cho th\u1ea5y r\u1eb1ng ph\u00e2n t\u1eed T-DNA \u0111i\u1ec1u khi\u1ec3n m\u1ed9t t\u00ednh tr\u1ea1ng n\u00e0o \u0111\u00f3 ho\u1eb7c nhi\u1ec1u t\u00ednh tr\u1ea1ng \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn l\u1ef1a trong qu\u00e1 tr\u00ecnh thu\u1ea7n h\u00f3a. Ph\u00e1t hi\u1ec7n n\u00e0y g\u00e2y s\u1ef1 ch\u00fa \u00fd \u0111\u1eb7c bi\u1ec7t v\u1ec1 t\u1ea7m quan tr\u1ecdng c\u1ee7a t\u01b0\u01a1ng t\u00e1c gi\u1eefa c\u00e2y tr\u1ed3ng v\u00e0 vi khu\u1ea9n.<\/p>\n

Ph\u00e1t hi\u1ec7n c\u00f2n cho th\u1ea5y khoai lang l\u00e0 lo\u00e0i c\u00e2y tr\u1ed3ng l\u00e0m ngu\u1ed3n l\u01b0\u01a1ng th\u1ef1c cho h\u00e0ng tri\u1ec7u ng\u01b0\u1eddi, n\u00f3 c\u00f3 th\u1ec3 thay \u0111\u1ed5i h\u1ec7 bi\u1ebfn h\u00f3a (paradigm) c\u1ee7a n\u00f3 \u0111\u1ec3 ki\u1ec3m so\u00e1t tr\u1ea1ng th\u00e1i \u201cunnatural\u201d (kh\u00f4ng c\u00f3 t\u00ednh ch\u1ea5t t\u1ef1 nhi\u00ean) c\u1ee7a lo\u00e0i c\u00e2y tr\u1ed3ng transgenic.<\/p>\n<\/div>\n

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Agrobacterium rhizogenes v\u00e0 Agrobacterium tumefaciens l\u00e0 nh\u1eefng vi khu\u1ea9n k\u00fd sinh th\u1ef1c v\u1eadt c\u00f3 kh\u1ea3 n\u0103ng l\u00e0m vector chuy\u1ec3n c\u00e1c \u0111o\u1ea1n ph\u00e2n t\u1eed DNA [transfer DNA (T-DNA)] mang c\u00e1c gen c\u00f3 ch\u1ee9c n\u0103ng mong mu\u1ed1n v\u00e0o genome c\u00e2y ch\u1ee7. C\u01a1 ch\u1ebf x\u1ea3y ra m\u1ed9t c\u00e1ch t\u1ef1 nhi\u00ean nh\u01b0 v\u1eady \u0111\u01b0\u1ee3c c\u00e1c nh\u00e0 c\u00f4ng ngh\u1ec7 sinh h\u1ecdc c\u00e2y tr\u1ed3ng \u00a0ph\u00e1t tri\u1ec3n ra c\u00e1c gi\u1ed1ng c\u00e2y tr\u1ed3ng bi\u1ebfn \u0111\u1ed5i gen hi\u1ec7n nay \u0111\u01b0\u1ee3c tr\u1ed3ng kho\u1ea3ng 10% di\u1ec7n t\u00edch \u0111\u1ea5t canh t\u00e1c c\u1ee7a th\u1ebf gi\u1edbi, cho d\u00f9 vi\u1ec7c s\u1eed d\u1ee5ng n\u00e0y c\u00f3 th\u1ec3 c\u00f3 k\u1ebft qu\u1ea3 tr\u00e1i chi\u1ec1u.<\/p>\n

Trong khi \u1ea5y ng\u01b0\u1eddi ta s\u1eed d\u1ee5ng c\u00e1c \u0111o\u1ea1n ph\u00e2n t\u1eed nh\u1ecf mang t\u00ednh ch\u1ea5t can thi\u1ec7p c\u00f3 thu\u1eadt ng\u1eef \u201csmall interfering RNAs\u201d, ho\u1eb7c siRNAs, c\u1ee7a c\u00e2y khoai lang ph\u1ee5c v\u1ee5 ph\u00e2n t\u00edch theo k\u1ef9 thu\u1eadt metagenomic, t\u00ednh ch\u1ea5t \u0111\u1ed3ng d\u1ea1ng theo tr\u00ecnh t\u1ef1 gen \u0111\u1ed1i v\u1edbi tr\u00ecnh t\u1ef1 c\u1ee7a T-DNA Agrobacterium spp. \u0111\u00e3 \u0111\u01b0\u1ee3c kh\u00e1m ph\u00e1. K\u1ef9 thu\u1eadt \u201csimple and quantitative PCR\u201d, ph\u00e2n t\u00edch Southern, th\u1ef1c hi\u1ec7n \u201cgenome walking\u201d, v\u00e0 s\u1eed d\u1ee5ng th\u01b0 vi\u1ec7n BAC (bacterial artificial chromosome library) \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng trong thanh l\u1ecdc v\u00e0 gi\u1ea3i tr\u00ecnh t\u1ef1 m\u1ed9t c\u00e1ch r\u00f5 r\u00e0ng. K\u1ebft qu\u1ea3 ch\u1ee9ng minh r\u1eb1ng hai v\u00f9ng T-DNA kh\u00e1c nhau (IbT-DNA1v\u00e0 IbT-DNA2) \u0111\u1ec1u th\u1ec3 hi\u1ec7n trong genome c\u1ee7a gi\u1ed1ng khoai lang tr\u1ed3ng tr\u1ecdt (Ipomoea batatas [L.] Lam.). Nh\u1eefng gen ngo\u1ea1i lai (foreign genes) n\u00e0y th\u1ec3 hi\u1ec7n \u1edf m\u1ee9c \u0111\u1ed9 c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c (detectable) trong c\u00e1c m\u00f4 kh\u00e1c nhau c\u1ee7a khoai lang. IbT-DNA1 \u0111\u01b0\u1ee3c t\u00ecm th\u1ea5y c\u00f3 t\u1ea5t c\u1ea3 b\u1ed1n khung \u0111\u1ecdc m\u00e3 gi\u1ed1ng nhau (ORFs: open reading frames) \u0111\u1ed1i v\u1edbi c\u00e1c gen tryptophan-2-monooxygenase (iaaM), indole-3-acetamide hydrolase (iaaH), C-protein (C-prot), v\u00e0 agrocinopine synthase (Acs) c\u1ee7a vi khu\u1ea9n Agrobacterium spp.<\/p>\n

IbT-DNA1 \u0111\u01b0\u1ee3c t\u00ecm th\u1ea5y trong t\u1ea5t c\u1ea3 291 gi\u1ed1ng khoai lang tr\u1ed3ng tr\u1ecdt kh\u1ea3o nghi\u1ec7m, nh\u01b0ng kh\u00f4ng g\u1ea7n v\u1edbi c\u00e1c lo\u00e0i hoang d\u1ea1i. IbT-DNA2 c\u00f3 ch\u1ee9a \u00edt nh\u1ea5t n\u0103m ORFs v\u1edbi s\u1ef1 \u0111\u1ed3ng d\u1ea1ng c\u00f3 \u00fd ngh\u0129a (homology) \u0111\u1ed1i v\u1edbi ORF14, ORF17n, locus qui \u0111\u1ecbnh t\u1ea1o r\u1ec5 (Rol)B\/RolC, ORF13, v\u00e0 ORF18\/ORF17n c\u1ee7a vi khu\u1ea9n A. rhizogenes. IbT-DNA2 \u0111\u01b0\u1ee3c t\u00ecm th\u1ea5y \u1edf 45 gi\u1ed1ng trong s\u1ed1 217 m\u1eabu gi\u1ed1ng khoai lang kh\u1ea3o nghi\u1ec7m v\u00e0 lo\u00e0i khoai lang hoang d\u1ea1i. Theo \u0111\u00f3, khoai lang \u0111\u00e3 \u0111\u01b0\u1ee3c chuy\u1ec3n gen c\u00f3 t\u00ednh ch\u1ea5t t\u1ef1 nhi\u00ean; trong khi n\u00f3 l\u00e0 lo\u00e0i l\u01b0\u01a1ng th\u1ef1c nu\u00f4i s\u1ed1ng h\u00e0ng tri\u1ec7u ng\u01b0\u1eddi, n\u00f3 c\u00f3 th\u1ec3 g\u00e2y \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn ng\u01b0\u1eddi ti\u00eau d\u00f9ng h\u00f4m nay, kh\u00f4ng nghi ng\u1edd g\u00ec n\u1eefa v\u1ec1 s\u1ef1 an to\u00e0n c\u1ee7a c\u00e2y tr\u1ed3ng transgenic.<\/p>\n

Xem chi ti\u1ebft: http:\/\/www.pnas.org\/content\/112\/18\/5844.abstract<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

T\u00e1c gi\u1ea3 th\u00f4ng b\u00e1o k\u1ebft qu\u1ea3 quan s\u00e1t \u0111\u00e1ng ch\u00fa \u00fd v\u1ec1 291 m\u1eabu gi\u1ed1ng khoai lang kh\u1ea3o nghi\u1ec7m, t\u1ea5t c\u1ea3 c\u00f3 m\u1ed9t ho\u1eb7c nhi\u1ec1u tr\u00ecnh t\u1ef1 c\u1ee7a transfer DNA (T-DNA). Nh\u1eefng tr\u00ecnh t\u1ef1 n\u00e0y, bi\u1ec3u hi\u1ec7n trong m\u1ed9t d\u00f2ng khoai lang v\u00f4 t\u00ednh (gi\u1ed1ng khoai lang \u201cHuachano\u201d). Gi\u1ed1ng n\u00e0y \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch chi ti\u1ebft […]\n","protected":false},"author":1,"featured_media":6326,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6712"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6712"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6712\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6326"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6712"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6712"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}