{"id":6757,"date":"2015-10-15T08:09:31","date_gmt":"2015-10-15T01:09:31","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1212"},"modified":"2015-10-15T08:09:31","modified_gmt":"2015-10-15T01:09:31","slug":"ho-gen-sweet-cua-dau-nanh","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/ho-gen-sweet-cua-dau-nanh\/","title":{"rendered":"H\u1ecd gen SWEET c\u1ee7a \u0111\u1eadu n\u00e0nh"},"content":{"rendered":"
SWEET (MtN3_saliva) domain proteins l\u00e0 m\u1ed9t nh\u00f3m protein v\u1eeba \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh g\u1ea7n \u0111\u00e2y thu\u1ed9c efflux transporters, c\u00f3 vai tr\u00f2 quan tr\u1ecdng s\u1ef1 ti\u1ebft ra \u0111\u01b0\u1eddng (sugar efflux), s\u1ef1 ch\u1ee9a nh\u1ef1a nguy\u00ean trong m\u00f4 libe (phloem loading), t\u01b0\u01a1ng t\u00e1c gi\u1eefa c\u00e2y ch\u1ee7 v\u00e0 pathogen v\u00e0 s\u1ef1 ph\u00e1t tri\u1ec3n m\u00f4 sinh d\u1ee5c. H\u1ecd gen SWEET \u0111\u01b0\u1ee3c \u01b0u ti\u00ean nghi\u00ean c\u1ee9u trong c\u00e2y Arabidopsis v\u00e0 nh\u1eefng th\u00e0nh vi\u00ean c\u1ee7a h\u1ecd gen n\u00e0y v\u1eeba \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u tr\u00ean c\u00e2y l\u00faa.<\/span><\/div>\n
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1. C\u01a1 s\u1edf khoa h\u1ecdc:<\/strong><\/p>\n

SWEET (MtN3_saliva) domain proteins l\u00e0 m\u1ed9t nh\u00f3m protein v\u1eeba \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh g\u1ea7n \u0111\u00e2y thu\u1ed9c efflux transporters, c\u00f3 vai tr\u00f2 quan tr\u1ecdng s\u1ef1 ti\u1ebft ra \u0111\u01b0\u1eddng (sugar efflux), s\u1ef1 ch\u1ee9a nh\u1ef1a nguy\u00ean trong m\u00f4 libe (phloem loading), t\u01b0\u01a1ng t\u00e1c gi\u1eefa c\u00e2y ch\u1ee7 v\u00e0 pathogen v\u00e0 s\u1ef1 ph\u00e1t tri\u1ec3n m\u00f4 sinh d\u1ee5c.
\nH\u1ecd gen SWEET \u0111\u01b0\u1ee3c \u01b0u ti\u00ean nghi\u00ean c\u1ee9u trong c\u00e2y Arabidopsis v\u00e0 nh\u1eefng th\u00e0nh vi\u00ean c\u1ee7a h\u1ecd gen n\u00e0y v\u1eeba \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u tr\u00ean c\u00e2y l\u00faa. Hi\u1ec7n nay, ng\u01b0\u1eddi ta v\u1eabn ch\u01b0a c\u00f3 m\u1ed9t ph\u00e2n t\u00edch n\u00e0o li\u00ean quan \u0111\u1ebfn transcriptome hay genomics c\u1ee7a nh\u1eefng gen SWEET trong c\u00e2y \u0111\u1eadu n\u00e0nh.<\/p>\n<\/div>\n

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2. K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u:<\/strong><\/p>\n

C\u00e1c t\u00e1c gi\u1ea3 \u0111\u00e3 khai th\u00e1c \u0111\u01b0\u1ee3c kh\u00eda c\u1ea1nh ti\u1ebfn h\u00f3a c\u1ee7a h\u1ecd gen SWEET trong nh\u1eefng lo\u00e0i c\u00e2y r\u1ea5t \u0111a d\u1ea1ng: t\u1eeb lo\u00e0i g\u1ed3m t\u1ea3o \u0111\u01a1n b\u00e0o cho \u0111\u1ebfn primitive single cell algae th\u1ef1c v\u1eadt h\u1ea1t k\u00edn (angiosperms) \u0111\u1eb7c bi\u1ec7t nh\u1ea5n m\u1ea1nh \u0111\u1ebfn lo\u00e0i \u0111\u1eadu n\u00e0nh (Glycine max). Nh\u1eefng \u0111\u1eb7c \u0111i\u1ec3m ti\u1ebfn h\u00f3a cho th\u1ea5y c\u00f3 s\u1ef1 lan r\u1ed9ng v\u00e0 t\u00e1i l\u1eadp (expansion and duplication) c\u1ee7a h\u1ecd gen SWEET c\u1ee7a th\u1ef1c v\u1eadt s\u1ed1ng tr\u00ean m\u1eb7t \u0111\u1ea5t. Nghi\u00ean c\u1ee9u t\u00ednh ch\u1ea5t \u0111\u1ed3ng d\u1ea1ng (homology) b\u1eb1ng c\u00f4ng c\u1ee5 BLAST v\u00e0 Hidden Markov Model-directed, th\u1ef1c hi\u1ec7n so s\u00e1nh chu\u1ed7i tr\u00ecnh t\u1ef1 (sequence alignments) x\u00e1c \u0111\u1ecbnh tr\u00ean 52 gen SWEET. Ng\u01b0\u1eddi ta \u0111\u00e3 th\u1ef1c hi\u1ec7n \u0111\u01b0\u1ee3c b\u1ea3n \u0111\u1ed3 gen tr\u00ean 15 nhi\u1ec5m s\u1eafc th\u1ec3 c\u1ee7a b\u1ed9 gen c\u00e2y \u0111\u1eadu n\u00e0nh nh\u01b0 nh\u1eefng s\u1ef1 ki\u1ec7n c\u00f3 t\u00ednh ch\u1ea5t t\u00e1i l\u1eadp theo chu tr\u00ecnh lu\u00e2n phi\u00ean (tandem duplication). Nh\u1eefng gen SWEET c\u1ee7a \u0111\u1eadu n\u00e0nh (GmSWEET) cho th\u1ea5y ch\u00fang c\u00f3 m\u1ed9t qu\u00e3ng th\u1ec3 hi\u1ec7n r\u1ea5t r\u1ed9ng (wide range of expression profiles) t\u1ea1i nh\u1eefng m\u00f4 t\u1ebf b\u00e0o kh\u00e1c nhau v\u00e0 \u1edf nh\u1eefng th\u1eddi k\u1ef3 ph\u00e1t tri\u1ec3n kh\u00e1c nhau.<\/p>\n

Ph\u00e2n t\u00edch d\u1eef li\u1ec7u transcriptome \u0111\u1eb7c tr\u01b0ng nh\u1ea5t v\u00e0 ph\u1ed5 th\u1ec3 hi\u1ec7n gen b\u1eb1ng k\u1ef9 thu\u1eadt quantitative real time PCR (qRT-PCR) cho th\u1ea5y: t\u00ednh chung nh\u1ea5t c\u1ee7a c\u00e1c gen GmSWEET \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh trong khi ph\u00e1t tri\u1ec3n m\u00f4 sinh d\u1ee5c. Nhi\u1ec1u th\u1ec3 bi\u1ebfn d\u1ecb di truy\u1ec1n t\u1ef1 nhi\u00ean (non-synonymous SNPs, premature stop codons and haplotype) \u0111\u01b0\u1ee3c ng\u01b0\u1eddi ta ph\u00e2n l\u1eadp trong c\u00e1c gen GmSWEET s\u1eed d\u1ee5ng to\u00e0n b\u1ed9 d\u1eef li\u1ec7u ch\u1ea1y tr\u00ecnh t\u1ef1 genome \u00a0v\u1edbi 106 gi\u1ed1ng \u0111\u1eadu n\u00e0nh. Ng\u01b0\u1eddi ta th\u1ea5y c\u00f3 s\u1ef1 k\u1ebft h\u1ee3p r\u1ea5t c\u00f3 \u00fd ngh\u0129a gi\u1eefa SNP-haplogroup v\u00e0 h\u00e0m l\u01b0\u1ee3ng sucrose h\u1ea1t \u0111\u1eadu n\u1eb1m trong 3 nh\u00f3m gen c\u1ee7a nhi\u1ec5m s\u1eafc th\u1ec3 6. K\u1ebft lu\u1eadn: Nghi\u00ean c\u1ee9u n\u00e0y \u0111\u00e3 s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p so s\u00e1nh genome (comparative genomics), transcriptome profiling v\u00e0 gi\u1ea3i tr\u00ecnh t\u1ef1 l\u1ea1i to\u00e0n b\u1ed9 genome.<\/p>\n

K\u1ebft qu\u1ea3 cung c\u1ea5p m\u1ed9t s\u1ef1 m\u00f4 t\u1ea3 c\u00f3 t\u00ednh h\u1ec7 th\u1ed1ng \u00a0nh\u1eefng gen SWEET c\u1ee7a \u0111\u1eadu n\u00e0nh v\u00e0 x\u00e1c \u0111\u1ecbnh nh\u1eefng gen \u1ee9ng c\u1eed vi\u00ean c\u00f3 t\u00ednh ch\u1ea5t gi\u1ea3 \u0111\u1ecbnh v\u1edbi ch\u1ee9c n\u0103ng c\u00f3 th\u1ec3 c\u00f3 trong s\u1ef1 ph\u00e1t tri\u1ec3n m\u00f4 sinh d\u1ee5c. Ph\u1ed5 th\u1ec3 hi\u1ec7n gen (expression profiling) t\u1ea1i nh\u1eefng giai \u0111o\u1ea1n ph\u00e1t tri\u1ec3n kh\u00e1c nhau v\u00e0 b\u1ed9 s\u1ed1 li\u1ec7u bi\u1ebfn thi\u00ean di truy\u1ec1n s\u1ebd gi\u00fap cho ch\u00fang ta nh\u01b0 m\u1ed9t ngu\u1ed3n th\u00f4ng tin quan tr\u1ecdng trong nghi\u00ean c\u1ee9u \u0111\u1eadu n\u00e0nh v\u00e0 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c xem l\u00e0 t\u00e0i li\u1ec7u r\u1ea5t c\u00f3 gi\u00e1 tr\u1ecb \u00a0\u0111\u1ec3 hi\u1ec5u \u0111\u01b0\u1ee3c s\u1ee9c ch\u1ee9a \u0111\u00e3 \u0111\u01b0\u1ee3c v\u1eadn h\u00e0nh nh\u01b0 th\u1ebf n\u00e0o (sink unloading) c\u0169ng nh\u01b0 vi\u1ec7c th\u00fac \u0111\u1ea9y s\u1ef1 gi\u1ea3i ph\u00f3ng ra carbohydrate \u0111\u1ec3 ph\u00e1t tri\u1ec3n h\u1ea1t nh\u1eb1m c\u1ea3i ti\u1ebfn n\u0103ng su\u1ea5t \u0111\u1eadu n\u00e0nh.<\/p>\n<\/div>\n

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Xem chi ti\u1ebft: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26162601<\/p>\n

\"\"<\/p>\n

H\u00ecnh:<\/u>\u00a0 Ph\u1ed5 th\u1ec3 hi\u1ec7n gen SWEET c\u1ee7a \u0111\u1eadu n\u00e0nh trong c\u00e1c m\u00f4 t\u1ebf b\u00e0o kh\u00e1c nhau<\/p>\n

Ngu\u1ed3n: Patil G, Valliyodan B, Deshmukh R, Prince S, Nicander B, Zhao M, Sonah H, Song L, Lin L, Chaudhary J, Liu Y, Joshi T, Xu D, Nguyen HT. 2015. Soybean (Glycine max) SWEET gene family: insights through comparative genomics, transcriptome profiling and whole genome re-sequence analysis. BMC Genomics. 2015 Jul 11; 16: 520.<\/span><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

SWEET (MtN3_saliva) domain proteins l\u00e0 m\u1ed9t nh\u00f3m protein v\u1eeba \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh g\u1ea7n \u0111\u00e2y thu\u1ed9c efflux transporters, c\u00f3 vai tr\u00f2 quan tr\u1ecdng s\u1ef1 ti\u1ebft ra \u0111\u01b0\u1eddng (sugar efflux), s\u1ef1 ch\u1ee9a nh\u1ef1a nguy\u00ean trong m\u00f4 libe (phloem loading), t\u01b0\u01a1ng t\u00e1c gi\u1eefa c\u00e2y ch\u1ee7 v\u00e0 pathogen v\u00e0 s\u1ef1 ph\u00e1t tri\u1ec3n m\u00f4 sinh d\u1ee5c. H\u1ecd gen SWEET \u0111\u01b0\u1ee3c […]\n","protected":false},"author":1,"featured_media":6027,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6757"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6757"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6757\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6027"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6757"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6757"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6757"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}