{"id":6758,"date":"2015-10-15T08:20:10","date_gmt":"2015-10-15T01:20:10","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1268"},"modified":"2015-10-15T08:20:10","modified_gmt":"2015-10-15T01:20:10","slug":"mot-so-ket-qua-nghien-cuu-lua-gm","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/mot-so-ket-qua-nghien-cuu-lua-gm\/","title":{"rendered":"M\u1ed9t s\u1ed1 k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u l\u00faa GM"},"content":{"rendered":"
\u0110\u1ea1i H\u1ecdc Kebangsaan, Malaysia v\u00e0\u00a0\u0110\u1ea1i H\u1ecdc N\u00f4ng Nghi\u1ec7p Th\u1ee5y \u0110i\u1ec3n m\u1edbi \u0111\u00e2y \u0111\u00e3 c\u00f4ng b\u1ed1 m\u1ed9t s\u1ed1 k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u li\u00ean quan \u0111\u1ebfn l\u00faa bi\u1ebfn \u0111\u1ed5i gen.\u00a0<\/span><\/div>\n
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1. L\u00faa bi\u1ebfn \u0111\u1ed5i gen n\u0103ng su\u1ea5t cao, \u00edt ph\u00e1t th\u1ea3i GHG:<\/strong><\/p>\n

C\u00e1c nh\u00e0 khoa h\u1ecdc thu\u1ed9c \u0110\u1ea1i H\u1ecdc N\u00f4ng Nghi\u1ec7p Th\u1ee5y \u0110i\u1ec3n \u00a0\u0111\u1ee9ng \u0111\u1ea7u l\u00e0 Chuanxin Sun v\u00e0 ctv. thu\u1ed9c nhi\u1ec1u c\u01a1 quan nghi\u00ean c\u1ee9u kh\u00e1c \u0111\u00e3 ph\u00e1t tri\u1ec3n th\u00e0nh c\u00f4ng c\u00e2y l\u00faa cao s\u1ea3n nh\u01b0ng \u00edt ph\u00e1t th\u1ea3i kh\u00ed nh\u00e0 k\u00ednh (GHG: greenhouse-gas \u00a0emissions). K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u quan tr\u1ecdng n\u00e0y \u0111\u01b0\u1ee3c c\u00f4ng b\u1ed1 tr\u00ean t\u1ea1p ch\u00ed Nature. Gi\u1ed1ng l\u00faa m\u1edbi n\u00e0y \u0111\u01b0\u1ee3c t\u1ea1o ra b\u1eb1ng c\u00f4ng ngh\u1ec7 di truy\u1ec1n \u0111\u1ec3 bi\u1ec3u hi\u1ec7n ra m\u1ed9t gen l\u1ea5y t\u1eeb l\u00faa m\u1ea1ch (barley). Gen \u1ea5y gi\u00fap c\u00e2y ph\u00e1t th\u1ea3i \u00edt h\u01a1n kh\u00edr methane v\u00e0 cho n\u0103ng su\u1ea5t h\u1ea1t t\u0103ng 43%. Theo Dr. Sun, ba n\u0103m so s\u00e1nh n\u0103ng su\u1ea5t \u0111\u1ec1u cho k\u1ebft qu\u1ea3 t\u00edch c\u1ef1c. S\u1ef1 gi\u1ea3m ph\u00e1t th\u1ea3i kh\u00ed methane cao nh\u1ea5t \u0111\u01b0\u1ee3c ng\u01b0\u1eddi ta quan s\u00e1t trong v\u1ee5 m\u00f9a H\u00c8, gi\u1ea3m t\u1eeb 0,3 \u0111\u1ebfn 10% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng. Gi\u1ed1ng l\u00faa GM n\u00e0y \u0111\u00e3 l\u00e0m gi\u1ea3m thi\u1ec3u ph\u00e1t th\u1ea3i kh\u00ed nh\u00e0 k\u00ednh r\u1ea5t \u0111\u00e1ng k\u1ec3 trong v\u1ee5 m\u00f9a THU, do nhi\u1ec7t \u0111\u1ed9 th\u1ea5p.<\/div>\n
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Xem MIT Technology Review. ho\u1eb7c t\u1ea1p ch\u00ed Nature.<\/p>\n

2.\u00a0S\u1ef1 bi\u1ec3u hi\u1ec7n m\u1ea1nh m\u1ebd c\u1ee7a protein li\u00ean quan \u0111\u1ebfn stress c\u1ee7a c\u00e2y l\u00faa ch\u1ed1ng ch\u1ecbu m\u1eb7n c\u00f3 trong c\u00e2y Arabidopsis<\/strong><\/p>\n<\/div>\n

H\u1ecd protein SAP (stress-associated protein) \u0111\u00e3 \u0111\u01b0\u1ee3c ng\u01b0\u1eddi ta t\u00ecm th\u1ea5y c\u00f3 ch\u1ee9c n\u0103ng ch\u1ed1ng ch\u1ecbu stress m\u1eb7n c\u1ee7a c\u00e2y tr\u1ed3ng. Zamri Zainal v\u00e0 ctv. thu\u1ed9c \u0110\u1ea1i H\u1ecdc Kebangsaan, Malaysia \u0111\u00e3 nghi\u00ean c\u1ee9u SAP MR219, m\u1ed9 th\u00e0nh vi\u00ean c\u1ee7a h\u1ecd SAP d9u77o5c k\u00edch ho\u1ea1t b\u1edfi stress m\u1eb7n g\u00e2y n\u00ean. Ph\u00e2n t\u00edch d\u00f2ng SAP MR219 cDNA \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb gi\u1ed1ng l\u00faa tr\u1ed3ng Oryza sativa var. MR219 cho th\u1ea5y: s\u1ea3n ph\u1ea9m c\u1ee7a gen n\u00e0y m\u00e3 h\u00f3a protein ch\u1ee9c n\u0103ng th\u00f4ng qua t\u01b0\u01a1ng t\u00e1c protein – protein. Gen SAP MR219 \u1ea5y \u0111\u01b0\u1ee3c ng\u01b0\u1eddi ta chuy\u1ec3n v\u00e0o c\u00e2y Arabidopsis thaliana v\u00e0 cho th\u1ebf hi\u1ec7n trong c\u00e2y m\u00f4 h\u00ecnh n\u00e0y, t\u1ea1o ra c\u00e1c d\u00f2ng Arabidopsis transgenic. Ng\u01b0\u1eddi ta \u0111\u00e1nh gi\u00e1 ph\u1ea3n \u1ee9ng ch\u1ed1ng ch\u1ecbu c\u1ee7a ch\u00fang trong \u0111i\u1ec1u ki\u1ec7n stress m\u1eb7n. \u1ede n\u1ed3ng \u0111\u1ed9 m\u1eb7n 250 mM NaCl, c\u00e1c d\u00f2ng transgenic nh\u01b0 v\u1eady \u0111\u00e3 n\u1ea9y m\u1ea7m kho\u1ea3ng 50% trong khi c\u00e2y nguy\u00ean th\u1ee7y (wild-type) h\u1ea7u nh\u01b0 kh\u00f4ng t\u0103ng tr\u01b0\u1edfng \u0111\u01b0\u1ee3c. K\u1ebft qu\u1ea3 cho th\u1ea5y gen SAP MR219 c\u00f3 th\u1ec3 \u0111\u00f3ng vai tr\u00f2 quan tr\u1ecdng trong th\u1ef1c v\u1eadt \u0111\u1ec3 ph\u1ea3n \u1ee9ng v\u1edbi stress m\u1eb7n. Xem Plant Omics Journal.
\n\"\"H\u00ecnh:S\u1ea3n ph\u1ea9m PCR theo k\u1ebft qu\u1ea3 \u0111i\u1ec7n di tr\u00ean agarose gel c\u1ee7a c\u00e2y Arabidopsis chuy\u1ec3n gen, s\u1eed d\u1ee5ng promoter primer 35S-F v\u00e0 SAP MR219OV-R \u0111\u1ec3 ph\u00e1t hi\u1ec7n ph\u00e2n t\u1eed c\u00f3 k\u00edch th\u01b0\u1edbc 700 bp. Lane M: 1 kp marker, Lane 1-5: putative transgenic plants, Lane 6: positive control, Lane 7: negative control (wild-type).<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u0110\u1ea1i H\u1ecdc Kebangsaan, Malaysia v\u00e0\u00a0\u0110\u1ea1i H\u1ecdc N\u00f4ng Nghi\u1ec7p Th\u1ee5y \u0110i\u1ec3n m\u1edbi \u0111\u00e2y \u0111\u00e3 c\u00f4ng b\u1ed1 m\u1ed9t s\u1ed1 k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u li\u00ean quan \u0111\u1ebfn l\u00faa bi\u1ebfn \u0111\u1ed5i gen.\u00a0 1. L\u00faa bi\u1ebfn \u0111\u1ed5i gen n\u0103ng su\u1ea5t cao, \u00edt ph\u00e1t th\u1ea3i GHG: C\u00e1c nh\u00e0 khoa h\u1ecdc thu\u1ed9c \u0110\u1ea1i H\u1ecdc N\u00f4ng Nghi\u1ec7p Th\u1ee5y \u0110i\u1ec3n \u00a0\u0111\u1ee9ng \u0111\u1ea7u l\u00e0 Chuanxin […]\n","protected":false},"author":1,"featured_media":1298,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6758"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6758"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6758\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/1298"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6758"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6758"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6758"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}