{"id":6770,"date":"2016-07-14T07:13:31","date_gmt":"2016-07-14T07:13:31","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=1029"},"modified":"2016-07-14T07:13:31","modified_gmt":"2016-07-14T07:13:31","slug":"phan-tich-di-truyen-huyet-thong-bang-ky-thuat-genome-wide-cua-ho-gen-cipk-trong-cay-san-va-su-the-hien-cua-cac-gen-dieu-khien-chong-chiu-kho-han","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/phan-tich-di-truyen-huyet-thong-bang-ky-thuat-genome-wide-cua-ho-gen-cipk-trong-cay-san-va-su-the-hien-cua-cac-gen-dieu-khien-chong-chiu-kho-han\/","title":{"rendered":"PH\u00c2N T\u00cdCH DI TRUY\u1ec0N HUY\u1ebeT TH\u1ed0NG B\u1eb0NG K\u1ef8 THU\u1eacT \u201cGenome-wide \u201c C\u1ee6A H\u1ecc GEN CIPK TRONG C\u00c2Y S\u1eaeN V\u00c0 S\u1ef0 TH\u1ec2 HI\u1ec6N C\u1ee6A C\u00c1C GEN \u0110I\u1ec0U KHI\u1ec2N CH\u1ed0NG CH\u1ecaU KH\u00d4 H\u1ea0N"},"content":{"rendered":"
S\u1eafn l\u00e0 c\u00e2y l\u01b0\u01a1ng th\u1ef1c quan tr\u1ecdng v\u00e0 l\u00e0 c\u00e2y cung c\u1ea5p nhi\u00ean li\u1ec7u sinh h\u1ecdc. S\u1eafn ch\u1ed1ng ch\u1ecbu \u0111\u01b0\u1ee3c nhi\u1ec1u lo\u1ea1i h\u00ecnh stress phi sinh h\u1ecdc. Tuy nhi\u00ean, c\u01a1 ch\u1ebf ch\u1ed1ng ch\u1ecbu \u1ea5y v\u1eabn ch\u01b0a \u0111\u01b0\u1ee3c bi\u1ebft r\u00f5 r\u00e0ng.<\/span><\/div>\n
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H\u1ecd protein kinase CIPKs (vi\u1ebft t\u1eaft t\u1eeb ch\u1eef: CBL-interacting protein kinases) c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong c\u00e1c ti\u1ebfn tr\u00ecnh ph\u00e1t tri\u1ec3n c\u1ee7a c\u00e2y, s\u1ef1 truy\u1ec1n t\u00edn hi\u1ec7u hormone, v\u00e0 \u0111\u00e1p \u1ee9ng v\u1edbi stress phi sinh h\u1ecdc. Tuy v\u1eady, ch\u01b0a c\u00f3 c\u01a1 s\u1edf d\u1eef li\u1ec7u n\u00e0o hi\u1ec7n nay cung c\u1ea5p nh\u1eefng ki\u1ebfn th\u1ee9c v\u1ec1 h\u1ecd CPK trong c\u00e2y s\u1eafn. Trong nghi\u00ean c\u1ee9u n\u00e0y, c\u00f3 25 CIPK gen \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh t\u1eeb genome c\u00e2y s\u1eafn tr\u00ean c\u01a1 s\u1edf d\u1eef li\u1ec7u c\u00f3 tr\u01b0\u1edbc \u0111\u00e2y v\u1ec1 gi\u1ea3i tr\u00ecnh t\u1ef1 genome.<\/p>\n

Nghi\u00ean c\u1ee9u di truy\u1ec1n huy\u1ebft th\u1ed1ng cho th\u1ea5y 25 MeCIPKs c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e2n l\u00e0m b\u1ed1n h\u1ecd ph\u1ee5 (subfamilies). C\u00e1c h\u1ecd ph\u1ee5 n\u00e0y \u0111\u01b0\u1ee3c xem x\u00e9t tr\u00ean c\u0103n c\u1ee9 nh\u1eefng c\u1ea5u tr\u00fac exon-intron v\u00e0 c\u1ea5u tr\u00fac c\u1ee7a motif c\u00e1c protein b\u1ea3o th\u1ee7. Ph\u00e2n t\u00edch transcriptomic m\u1ed9t h\u1ecd ph\u1ee5 nguy\u00ean th\u1ee7y v\u00e0 hai gi\u1ed1ng s\u1eafn \u0111ang canh t\u00e1c cho th\u1ea5y h\u1ea7u h\u1ebft MeCIPKs bi\u1ec3u hi\u1ec7n kh\u00e1c bi\u1ec7t nhau gi\u1eefa c\u00e1c h\u1ecd ph\u1ee5 v\u00e0 gi\u1eefa c\u00e1c gi\u1ed1ng s\u1eafn canh t\u00e1c trong nh\u1eefng m\u00f4 kh\u00e1c nhau ho\u1eb7c c\u00f3 nh\u1eefng ph\u1ea3n \u1ee9ng kh\u00e1c nhau \u0111\u1ed1i v\u1edbi stress kh\u00f4 h\u1ea1n. M\u1ed9t v\u00e0i gen t\u01b0\u01a1ng \u0111\u1ed3ng (orthologous) li\u00ean quan \u0111\u1ebfn t\u01b0\u01a1ng t\u00e1c c\u1ee7a CIPK c\u0169ng \u0111\u01b0\u1ee3c ng\u01b0\u1eddi ta x\u00e1c \u0111\u1ecbnh gi\u1eefa b\u1ed9 gen c\u00e2y Arabidopsis v\u00e0 s\u1eafn. C\u00e1c h\u1ec7 th\u1ed1ng t\u01b0\u01a1ng t\u00e1c v\u00e0 c\u00e1c h\u1ee3p ph\u1ea7n li\u00ean quan \u0111\u1ebfn s\u1ef1 ki\u1ec7n \u0111\u1ed3ng th\u1ec3 hi\u1ec7n c\u1ee7a nh\u1eefng gen \u0111\u1ed3ng d\u1ea1ng \u1ea5y cho th\u1ea5y r\u1eb1ng: nh\u1eefng chu tr\u00ecnh c\u1ea7n thi\u1ebft \u0111\u01b0\u1ee3c ki\u1ec3m so\u00e1t b\u1edfi h\u1ec7 th\u1ed1ng CIPK c\u00f3 th\u1ec3 c\u00f3 trong ph\u1ea3n \u1ee9ng chuy\u00ean bi\u1ec7t \u0111\u1ed1i v\u1edbi stress kh\u00f4 h\u1ea1n trong nhi\u1ec5u m\u1eabu gi\u1ed1ng s\u1eafn thu th\u1eadp. Ch\u00edn gen MeCIPK \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn l\u1ecdc \u0111\u1ec3 nghi\u00ean c\u1ee9u ph\u1ea3n \u1ee9ng phi\u00ean m\u00e3 \u0111\u1ed1i v\u1edbi c\u00e1c ch\u1ea5t k\u00edch th\u00edch kh\u00e1c nhau; k\u1ebft qu\u1ea3 cho th\u1ea5y c\u00f3 m\u1ed9t ph\u1ea3n \u1ee9ng kh\u00e1 r\u00f5 c\u1ee7a nh\u1eefng gen tr\u1eafc nghi\u1ec7m MeCIPK \u0111\u1ed1i v\u1edbi s\u1ef1 ki\u1ec7n li\u00ean quan \u0111\u1ebfn \u00e1p su\u1ea5t th\u1ea9m th\u1ea5u, m\u1eb7n, l\u1ea1nh, c\u00e1c stress li\u00ean quan \u0111\u1ebfn oxidative, v\u00e0 truy\u1ec1n t\u00edn hi\u1ec7u ABA.<\/p>\n

S\u1ef1 x\u00e1c \u0111\u1ecbnh v\u00e0 ph\u00e2n t\u00edch th\u1ec3 hi\u1ec7n c\u1ee7a h\u1ecd CIPK cho th\u1ea5y h\u1ecd gen CIPK l\u00e0 nh\u1eefng th\u00e0nh ph\u1ea7n v\u00f4 c\u00f9ng quan tr\u1ecdng c\u1ee7a s\u1ef1 ph\u00e1t tri\u1ec3n v\u00e0 nh\u1eefng l\u1ed9 tr\u00ecnh truy\u1ec1n t\u00edn hi\u1ec7u c\u1ee7a c\u00e2y s\u1eafn. Nghi\u00ean c\u1ee9u n\u00e0y s\u1ebd gi\u00fap cho ch\u00fang ta hi\u1ec3u \u0111\u01b0\u1ee3c b\u1ea3n ch\u1ea5t c\u1ee7a h\u1ecd gen CIPK v\u00e0 cung c\u1ea5p nh\u1eefng hi\u1ec3u bi\u1ebft v\u1ec1 c\u00e1c ph\u1ea3n \u1ee9ng v\u1ec1 stress phi sinh h\u1ecdc c\u0169ng nh\u01b0 s\u1ef1 truy\u1ec1n t\u00edn hi\u1ec7u trong b\u1ed9 gen c\u00e2y s\u1eafn.<\/p>\n<\/div>\n

\"image005(2)\"<\/a><\/div>\n
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H\u00ecnh 1: Ph\u00e2n t\u00edch di truy\u1ec1n huy\u1ebft th\u1ed1ng (phylogenetic analysis) CIPK proteins trong c\u00e2y s\u1eafn, l\u00faa, Arabidopsis, v\u00e0 Populus<\/strong>.<\/p>\n

C\u00f3 t\u1ed5ng s\u1ed1 25 CIPK proteins c\u1ee7a s\u1eafn (h\u00ecnh tam gi\u00e1c), 26 c\u1ee7a Arabidopsis (h\u00ecnh vu\u00f4ng), 34 c\u1ee7a l\u00faa (h\u00ecnh tr\u00f2n) v\u00e0 27 c\u1ee7a Populus (h\u00ecnh thoi) \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u1ea1o ra c\u00e2y gia h\u1ec7 theo d\u1ea1ng \u201cmaximum likelihood\u201d tr\u00ean ph\u1ea7n m\u1ec1m ClustalX 2.0 v\u00e0 MEGA5 v\u1edbi 1000 bootstrap. C\u00e1c nh\u00e1nh v\u1edbi \u00edt h\u01a1n 50% \u201cbootstrap support\u201d \u0111\u00e3 ch\u1ed3ng l\u00ean nhau. N\u0103m nh\u00f3m di truy\u1ec1n \u0111\u00e3 \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng ch\u1eef A, B, C, D, v\u00e0 E.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

S\u1eafn l\u00e0 c\u00e2y l\u01b0\u01a1ng th\u1ef1c quan tr\u1ecdng v\u00e0 l\u00e0 c\u00e2y cung c\u1ea5p nhi\u00ean li\u1ec7u sinh h\u1ecdc. S\u1eafn ch\u1ed1ng ch\u1ecbu \u0111\u01b0\u1ee3c nhi\u1ec1u lo\u1ea1i h\u00ecnh stress phi sinh h\u1ecdc. Tuy nhi\u00ean, c\u01a1 ch\u1ebf ch\u1ed1ng ch\u1ecbu \u1ea5y v\u1eabn ch\u01b0a \u0111\u01b0\u1ee3c bi\u1ebft r\u00f5 r\u00e0ng.<\/p>\n","protected":false},"author":1,"featured_media":6894,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6770"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6770"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6770\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6894"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6770"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6770"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6770"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}