{"id":6784,"date":"2017-02-19T09:45:30","date_gmt":"2017-02-19T02:45:30","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=4881"},"modified":"2017-02-19T09:45:30","modified_gmt":"2017-02-19T02:45:30","slug":"thu-nghiem-chuyen-gen-dna-helicase-45-pdg45-tai-vien-nghien-cuu-lua-bangladesh","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/thu-nghiem-chuyen-gen-dna-helicase-45-pdg45-tai-vien-nghien-cuu-lua-bangladesh\/","title":{"rendered":"Th\u1eed nghi\u1ec7m chuy\u1ec3n gen DNA Helicase 45 (PDG45) t\u1ea1i Vi\u1ec7n nghi\u00ean c\u1ee9u l\u00faa Bangladesh"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">Chuy\u1ec3n gen DNA Helicase 45 bi\u1ec3u hi\u1ec7n \u1edf ki\u1ec3u gen l\u00faa\u00a0v\u00e0 m\u1ee9c \u0111\u1ed9 ch\u1ecbu m\u1eb7n trong ki\u1ec1m ch\u1ebf sinh h\u1ecdc. \u0110\u1ed9 m\u1eb7n \u0111\u1ea5t l\u00e0 y\u1ebfu t\u1ed1 h\u1ea1n ch\u1ebf \u0111\u1ea7u ti\u00ean \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 t\u0103ng tr\u01b0\u1edfng v\u00e0 s\u1ea3n xu\u1ea5t g\u1ea1o\u00a0tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. DNA helicase (<em>PDH45<\/em>) t\u1eeb c\u00e2y \u0111\u1eadu (<em>Pisum sativum<\/em>), l\u00e0 m\u1ed9t th\u00e0nh vi\u00ean c\u1ee7a\u00a0DEAD-box protein family v\u00e0 \u0111\u00f3ng m\u1ed9t vai tr\u00f2 quan tr\u1ecdng trong vi\u1ec7c ki\u1ec3m so\u00e1t abiotic stress tolerance trong th\u1ef1c v\u1eadt.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">S\u1ef1 bi\u1ec3u hi\u1ec7n qu\u00e1 m\u1ee9c <em>PDH45<\/em> trao \u0111\u1ed5i c\u1ea3 c\u00e2y gi\u1ed1ng v\u00e0 sinh s\u1ea3n\u00a0c\u00f3 kh\u1ea3 n\u0103ng ch\u1ed1ng ch\u1ecbu m\u1eb7n \u1edf giai \u0111o\u1ea1n \u0111\u1ea7u c\u1ee7a gi\u1ed1ng l\u00faa g\u1ea1o Bangladesh, Binnatoa (BA). Trong nghi\u00ean c\u1ee9u hi\u1ec7n t\u1ea1i,\u00a0chuy\u1ec3n gen BA ch\u1ee9a <em>PDH4<\/em>5 \u0111\u00e3 \u0111\u01b0\u1ee3c lai v\u1edbi hai gi\u1ed1ng g\u1ea1o n\u00f4ng d\u00e2n tr\u1ed3ng kh\u00e1c nhau ph\u1ed5 bi\u1ebfn BRRI\u00a0(Bangladesh Rice Research Institute), BRRI dhan28 (BR28) v\u00e0 BRRI dhan47 (BR47) trong m\u1ed9t nh\u00e0 l\u01b0\u1edbi c\u00f3 ch\u1ee9a \u1edf DU. \u1ede\u00a0th\u1ebf h\u1ec7 F1\u00a0th\u1ef1c v\u1eadt c\u00f3 ki\u1ec3u h\u00ecnh th\u1ee5 th\u1ec3 ch\u1ee9a transgene \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn b\u1eb1ng ph\u00e2n t\u1eed\u00a0th\u1eed nghi\u1ec7m v\u00e0 n\u00e2ng cao t\u1eeb F1 \u0111\u1ebfn F5. <\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">Bi\u1ec3u hi\u1ec7n c\u1ee7a <em>PDH45<\/em> \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf t\u1ea5t c\u1ea3 c\u00e1c th\u1ebf h\u1ec7. \u0110\u00e3 c\u00f3 m\u1ed9t s\u1ed1 kh\u00e1c bi\u1ec7t v\u1ec1 m\u1ee9c bi\u1ec3u hi\u1ec7n PDH45 trong c\u00e1c ki\u1ec3u gen cho\u00a0v\u00e0 nh\u1eadn\u00a0m\u00e0 kh\u00f4ng c\u00f3 b\u1ea5t k\u1ef3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn kh\u1ea3 n\u0103ng\u00a0\u0111\u1ed9 m\u1eb7n\u00a0c\u1ee7a ch\u00fang trong c\u00e1c th\u1eed nghi\u1ec7m kh\u00e1c nhau. T\u1ea5t c\u1ea3 c\u00e1c ki\u1ec3u gen l\u00faa\u00a0cho th\u1ea5y t\u0103ng tr\u01b0\u1edfng m\u1ea1nh, h\u00e0m l\u01b0\u1ee3ng chlorophyll cao h\u01a1n, ch\u1ea5t \u0111i\u1ec7n ph\u00e2n th\u1ea5p h\u01a1n v\u00e0 th\u1ea5p h\u01a1n \u0111i\u1ec3m SES so v\u1edbi c\u00e1c lo\u1ea1i hoang d\u00e3 t\u01b0\u01a1ng \u1ee9ng. \u1ede giai \u0111o\u1ea1n sinh s\u1ea3n theo\u00a0\u00e1p l\u1ef1c m\u1eb7n li\u00ean t\u1ee5c, c\u00e1c d\u00f2ng lai t\u1ea1o (BR28 v\u00e0 BR47) cho th\u1ea5y t\u1ed1t h\u01a1n \u0111\u00e1ng k\u1ec3\u00a0kh\u1ea3 n\u0103ng sinh s\u1ea3n v\u00e0 n\u0103ng su\u1ea5t tr\u00ean m\u1ed9t c\u00e2y tr\u1ed3ng so v\u1edbi c\u00e1c lo\u1ea1i hoang d\u00e3 t\u01b0\u01a1ng \u1ee9ng. Ki\u1ec3m tra\u00a0c\u00e1c t\u01b0\u01a1ng t\u00e1c c\u1ee7a gen chuy\u1ec3n gen <em>PDH45<\/em> trong t\u1ea5t c\u1ea3 c\u00e1c ki\u1ec3u gen l\u00faa v\u00e0 s\u1ef1 bi\u1ec3u hi\u1ec7n c\u1ee7a s\u00e1u gen li\u00ean quan \u0111\u1ebfn\u00a0\u0111\u1ed9 ch\u1ecbu\u00a0m\u1eb7n\u00a0t\u0103ng \u0111\u00e1ng k\u1ec3 \u1edf m\u1ee9c NaCl 150 mM.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">C\u00e1c d\u00f2ng chuy\u1ec3n gen \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn c\u1ee7a ch\u00fang t\u00f4i \u0111\u00e3 \u0111\u01b0\u1ee3c g\u1eedi t\u1edbi BRRI theo th\u1ee7 t\u1ee5c v\u00e0 c\u1ea5p ph\u00e9p v\u1ec1 an to\u00e0n sinh h\u1ecdc\u00a0\u0111\u1ec3 ki\u1ec3m tra v\u1ec1 hi\u1ec7u qu\u1ea3 c\u1ee7a ch\u00fang trong nh\u00e0 k\u00ednh.\u00a0<\/span><\/p>\n<p style=\"text-align: right;\"><em><span style=\"font-family: 'times new roman', times, serif;\">Ngu\u1ed3n: ilsirf.org<\/span><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chuy\u1ec3n gen DNA Helicase 45 bi\u1ec3u hi\u1ec7n \u1edf ki\u1ec3u gen l\u00faa\u00a0v\u00e0 m\u1ee9c \u0111\u1ed9 ch\u1ecbu m\u1eb7n trong ki\u1ec1m ch\u1ebf sinh h\u1ecdc. \u0110\u1ed9 m\u1eb7n \u0111\u1ea5t l\u00e0 y\u1ebfu t\u1ed1 h\u1ea1n ch\u1ebf \u0111\u1ea7u ti\u00ean \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 t\u0103ng tr\u01b0\u1edfng v\u00e0 s\u1ea3n xu\u1ea5t g\u1ea1o\u00a0tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. DNA helicase (PDH45) t\u1eeb c\u00e2y \u0111\u1eadu (Pisum sativum), l\u00e0 m\u1ed9t th\u00e0nh vi\u00ean [&#8230;]\n","protected":false},"author":1,"featured_media":6527,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6784"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6784"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6784\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6527"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6784"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6784"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6784"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}