K\u1ef9 thu\u1eadt t\u00e1i b\u1ea3n \u0111\u1eb3ng nhi\u1ec7t (LAMP –\u00a0LOOP MEDIATED ISOTHERMAL AMPLIFICATION) l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p t\u00e1i b\u1ea3n acid nucleic (DNA ho\u1eb7c RNA) trong m\u1ed9t nhi\u1ec7t \u0111\u1ed9 duy nh\u1ea5t s\u1eed d\u1ee5ng m\u1ed9t b\u1ed9 m\u1ed3i \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1eb7c bi\u1ec7t v\u00e0 m\u1ed9t DNA polymerase c\u00f3 kh\u1ea3 n\u0103ng v\u1eeba t\u00e1ch m\u1ea1ch \u0111\u00f4i v\u1eeba t\u1ed5ng h\u1ee3p. S\u1ea3n ph\u1ea9m DNA l\u00e0 c\u1ea5u tr\u00fac g\u1ed1c v\u00f2ng do s\u1ef1 l\u1eb7p l\u1ea1i nhi\u1ec1u l\u1ea7n c\u1ee7a gen \u0111\u00edch. LAMP l\u00e0 ph\u01b0\u01a1ng ph\u00e1p c\u00f3 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao, hi\u1ec7u qu\u1ea3, nhanh ch\u00f3ng v\u00e0 s\u1ea3n ph\u1ea9m c\u00f3 th\u1ec3 quan s\u00e1t tr\u1ef1c ti\u1ebfp trong \u1ed1ng ph\u1ea3n \u1ee9ng, do v\u1eady ph\u01b0\u01a1ng ph\u00e1p LAMP c\u00f3 th\u1ec3 l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p \u0111\u1eb7c bi\u1ec7t h\u1eefu \u00edch \u0111\u1ec3 ch\u1ea9n \u0111o\u00e1n b\u1ec7nh truy\u1ec1n nhi\u1ec5m nhi\u1ec5m nh\u01b0 virus, vi sinh v\u1eadt,….<\/p>\n
\u0110\u1ed1i v\u1edbi \u0111\u1eadu t\u01b0\u01a1ng GMO, theo Liu M v\u00e0 c\u1ed9ng s\u1ef1, 2009 (ngu\u1ed3n: ncbi.nlm.nih.gov) nghi\u00ean c\u1ee9u ch\u1ec9 ra r\u1eb1ng s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p LAMP, m\u1ed9t h\u1ec7 th\u1ed1ng d\u00f2 t\u00ecm nh\u1ea1y c\u1ea3m \u0111\u1eb7c bi\u1ec7t v\u00e0 nh\u1ea1y c\u1ea3m cao \u0111\u1ed1i v\u1edbi \u0111\u1eadu t\u01b0\u01a1ng bi\u1ebfn \u0111\u1ed5i gen (Roundup Ready) \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf.<\/p>\n
Trong h\u1ec7 th\u1ed1ng ph\u00e1t hi\u1ec7n n\u00e0y, m\u1ed9t b\u1ed9 b\u1ed1n m\u1ed3i \u0111\u00e3 \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf b\u1eb1ng c\u00e1ch nh\u1eafm m\u1ee5c ti\u00eau gen ngo\u1ea1i sinh 35S EPSPS.\u00a0DNA \u0111\u01b0\u1ee3c khu\u1ebfch \u0111\u1ea1i v\u00e0 th\u1ec3 hi\u1ec7n c\u00e1c b\u0103ng tr\u00ean gel agarose trong v\u00f2ng 45 ph\u00fat \u1edf \u0111i\u1ec1u ki\u1ec7n \u0111\u1eb3ng nhi\u1ec7t \u1edf 65 \u0111\u1ed9 C. Kh\u00f4ng c\u00f3 \u0111i\u1ec7n di gel, LAMP amplcon th\u1ea5y \u0111\u01b0\u1ee3c tr\u1ef1c ti\u1ebfp trong \u1ed1ng ph\u1ea3n \u1ee9ng b\u1eb1ng c\u00e1ch th\u00eam SYBR Green I (N’,N’-dimethyl-N-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N-propylpropane-1,3-diamine) \u0111\u1ec3 ki\u1ec3m tra m\u1eaft th\u01b0\u1eddng.\u00a0\u0110\u1ed9 nh\u1ea1y ph\u00e1t hi\u1ec7n c\u1ee7a LAMP cao g\u1ea5p 10 l\u1ea7n so v\u1edbi ph\u01b0\u01a1ng ph\u00e1p PCR \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp trong ph\u00f2ng th\u00ed nghi\u1ec7m.<\/p>\n
H\u01a1n n\u1eefa, ph\u01b0\u01a1ng ph\u00e1p LAMP nhanh h\u01a1n nhi\u1ec1u, ch\u1ec9 m\u1ea5t 70 ph\u00fat, so v\u1edbi 300 ph\u00fat \u0111\u1ed1i v\u1edbi ph\u01b0\u01a1ng ph\u00e1p PCR \u0111\u1ec3 ho\u00e0n th\u00e0nh vi\u1ec7c ph\u00e2n t\u00edch \u0111\u1eadu t\u01b0\u01a1ng GM.\u00a0So v\u1edbi ph\u01b0\u01a1ng ph\u00e1p PCR truy\u1ec1n th\u1ed1ng, quy tr\u00ecnh th\u1ef1c hi\u1ec7n LAMP nhanh h\u01a1n v\u00e0 nh\u1ea1y h\u01a1n,\u00a0kh\u00f4ng c\u1ea7n m\u00e1y PCR \u0111\u1eb7c bi\u1ec7t ho\u1eb7c thi\u1ebft b\u1ecb \u0111i\u1ec7n di.\u00a0Do \u0111\u00f3, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 th\u1ec3 l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 r\u1ea5t h\u1eefu \u00edch cho ph\u00e1t hi\u1ec7n GMO v\u00e0 \u0111\u1eb7c bi\u1ec7t thu\u1eadn ti\u1ec7n cho vi\u1ec7c s\u00e0ng l\u1ecdc nhanh. Do v\u1eady, LAMP th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u1ea1o c\u00e1c b\u1ed9 kit ch\u1ea9n \u0111o\u00e1n nhanh \u0111ang \u0111\u01b0\u1ee3c ph\u1ed5 bi\u1ebfn r\u1ed9ng r\u00e3i tr\u00ean th\u1ebf gi\u1edbi.<\/p>\n
Ngu\u1ed3n: ncbi.nlm.nih.gov<\/em><\/p>\n","protected":false},"excerpt":{"rendered":" K\u1ef9 thu\u1eadt t\u00e1i b\u1ea3n \u0111\u1eb3ng nhi\u1ec7t (LAMP –\u00a0LOOP MEDIATED ISOTHERMAL AMPLIFICATION) l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p t\u00e1i b\u1ea3n acid nucleic (DNA ho\u1eb7c RNA) trong m\u1ed9t nhi\u1ec7t \u0111\u1ed9 duy nh\u1ea5t s\u1eed d\u1ee5ng m\u1ed9t b\u1ed9 m\u1ed3i \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1eb7c bi\u1ec7t v\u00e0 m\u1ed9t DNA polymerase c\u00f3 kh\u1ea3 n\u0103ng v\u1eeba t\u00e1ch m\u1ea1ch \u0111\u00f4i v\u1eeba t\u1ed5ng h\u1ee3p. S\u1ea3n ph\u1ea9m DNA […]\n","protected":false},"author":1,"featured_media":6213,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6806"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6806"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6806\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6213"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6806"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6806"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6806"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}