{"id":6841,"date":"2018-02-05T23:51:11","date_gmt":"2018-02-05T16:51:11","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=4967"},"modified":"2018-02-05T23:51:11","modified_gmt":"2018-02-05T16:51:11","slug":"xac-dinh-phan-ho-nhom-iia-wrky-va-phan-tich-chuc-nang-cua-ghwrky17-tren-cay-bong-vung-cao-gossypium-hirsutum-l-tai-trung-quoc","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/xac-dinh-phan-ho-nhom-iia-wrky-va-phan-tich-chuc-nang-cua-ghwrky17-tren-cay-bong-vung-cao-gossypium-hirsutum-l-tai-trung-quoc\/","title":{"rendered":"X\u00e1c \u0111\u1ecbnh ph\u00e2n h\u1ecd nh\u00f3m IIA WRKY v\u00e0 ph\u00e2n t\u00edch ch\u1ee9c n\u0103ng c\u1ee7a GhWRKY17 tr\u00ean c\u00e2y B\u00f4ng v\u00f9ng cao (Gossypium hirsutum L.) t\u1ea1i Trung Qu\u1ed1c"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif; font-size: 12pt;\">B\u00f4ng l\u00e0 m\u1ed9t lo\u1ea1i c\u00e2y tr\u1ed3ng kinh t\u1ebf quan tr\u1ecdng v\u00e0 nguy\u00ean li\u1ec7u d\u1ec7t c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a n\u1ec1n kinh t\u1ebf qu\u1ed1c d\u00e2n. Nh\u1eefng ph\u00e1t hi\u1ec7n tr\u01b0\u1edbc \u0111\u00e2y \u0111\u00e3 ch\u1ec9 ra r\u1eb1ng stress v\u00e0 gi\u00e0 h\u00f3a l\u00e0 nh\u1eefng y\u1ebfu t\u1ed1 quan tr\u1ecdng h\u1ea1n ch\u1ebf s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a b\u00f4ng, ch\u1ea5t l\u01b0\u1ee3ng s\u1ee3i v\u00e0 n\u0103ng su\u1ea5t. Cho \u0111\u1ebfn nay, ph\u00e2n t\u00edch ch\u1ee9c n\u0103ng c\u1ee7a nh\u00f3m IIa GhWRKYs ch\u1ee7 y\u1ebfu t\u1eadp trung v\u00e0o c\u00e1c \u0111i\u1ec1u ki\u1ec7n stress v\u1edbi ph\u00e2n t\u00edch h\u1ea1n ch\u1ebf v\u1ec1 vai tr\u00f2 c\u00f3 th\u1ec3 c\u1ee7a ch\u00fang trong s\u1ef1 gi\u00e0 h\u00f3a \u1edf l\u00e1. Nh\u00f3m nghi\u00ean c\u1ee9u ph\u00e2n t\u00edch TFA nh\u00f3m IIa trong b\u00f4ng v\u00f9ng cao li\u00ean quan \u0111\u1ebfn ph\u00e1t sinh lo\u00e0i, c\u1ea5u tr\u00fac intron-exon, th\u00e0nh ph\u1ea7n, t\u1ef7 l\u1ec7 Ka\/Ks v\u00e0 c\u00e1c m\u1eabu bi\u1ec3u hi\u1ec7n trong c\u00e1c m\u00f4 kh\u00e1c nhau v\u00e0 trong th\u1eddi k\u1ef3 gi\u00e0 h\u00f3a l\u00e1. \u0110\u1ec3 ph\u00e2n t\u00edch s\u00e2u h\u01a1n ch\u1ee9c n\u0103ng c\u1ee7a gen WRA nh\u00f3m IIa, GhWRKY17 \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00e1ch ly v\u00e0 m\u00f4 t\u1ea3. Bi\u1ec3u hi\u1ec7n GhWRKY17 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c g\u00e2y ra trong qu\u00e1 tr\u00ecnh gi\u00e0 h\u00f3a l\u00e1 v\u00e0 bi\u1ec3u hi\u1ec7n qu\u00e1 m\u1ee9c c\u1ee7a GhWRKY17 d\u1eabn \u0111\u1ebfn m\u1ed9t ki\u1ec3u h\u00ecnh gi\u00e0 h\u00f3a s\u1edbm trong c\u00e2y Arabidopsis chuy\u1ec3n gen.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif; font-size: 12pt;\">\u0110\u1ec3 \u0111\u00e1nh gi\u00e1 c\u00e1c m\u1eabu bi\u1ec3u hi\u1ec7n gen trong c\u00e1c m\u00f4 kh\u00e1c nhau v\u00e0 trong qu\u00e1 tr\u00ecnh l\u00e3o h\u00f3a l\u00e1 t\u1ef1 nhi\u00ean, gi\u1ed1ng CCRI74 gi\u00e0 h\u00f3a s\u1edbm v\u00e0 gi\u1ed1ng Liao 4086 kh\u00f4ng gi\u00e0 h\u00f3a s\u1edbm \u0111\u01b0\u1ee3c tr\u1ed3ng trong khu v\u1ef1c b\u00f4ng t\u1ea1i Vi\u1ec7n Nghi\u00ean c\u1ee9u B\u00f4ng c\u1ee7a CAAS (Anyang, Henan, Trung Qu\u1ed1c) ). \u0110\u1ec3 ph\u00e2n t\u00edch bi\u1ec3u hi\u1ec7n m\u00f4, c\u00e1c m\u00f4 bao g\u1ed3m r\u1ec5, th\u00e2n, l\u00e1, c\u00e1nh hoa, nh\u1ee5y hoa, nh\u1ecb hoa, no\u00e3n v\u00e0 s\u1ee3i \u0111\u01b0\u1ee3c thu th\u1eadp t\u1eeb CCRI74. \u0110\u1ec3 ph\u00e1t hi\u1ec7n bi\u1ec3u hi\u1ec7n gen trong qu\u00e1 tr\u00ecnh gi\u00e0 h\u00f3a l\u00e1 t\u1ef1 nhi\u00ean, hai ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 thu th\u1eadp m\u1eabu. \u0110\u1ec3 ki\u1ec3m tra c\u00e1c giai \u0111o\u1ea1n ph\u00e1t tri\u1ec3n l\u00e1 kh\u00e1c nhau, th\u1ee9 t\u01b0 h\u00e0ng \u0111\u1ea7u l\u00e1 t\u1eeb hai gi\u1ed1ng \u0111\u01b0\u1ee3c l\u1ea5y m\u1eabu m\u1ed7i 10 ng\u00e0y sau khi gieo 80 ng\u00e0y. \u0110\u1ec3 ki\u1ec3m tra c\u00e1c l\u00e1 kh\u00e1c nhau, n\u0103m giai \u0111o\u1ea1n kh\u00e1c nhau c\u1ee7a l\u00e1 \u0111\u01b0\u1ee3c thu t\u1eeb CCRI74: Giai \u0111o\u1ea1n 1, m\u1ed9t l\u00e1 m\u1edbi m\u1edf r\u1ed9ng; Giai \u0111o\u1ea1n 2, l\u00e1 tr\u01b0\u1edfng th\u00e0nh; Giai \u0111o\u1ea1n 3, 25% di\u1ec7n t\u00edch l\u00e1 gi\u00e0 h\u00f3a; Giai \u0111o\u1ea1n 4, 50% di\u1ec7n t\u00edch l\u00e1 gi\u00e0 h\u00f3a; v\u00e0 Giai \u0111o\u1ea1n 5, l\u00e1 c\u00f3 \u00edt nh\u1ea5t 75% di\u1ec7n t\u00edch gi\u00e0 h\u00f3a.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif; font-size: 12pt;\">C\u00e1c y\u1ebfu t\u1ed1 phi\u00ean m\u00e3 WRKY \u0111\u00f3ng vai tr\u00f2 quan tr\u1ecdng trong vi\u1ec7c b\u1ea3o v\u1ec7 th\u1ef1c v\u1eadt, ph\u1ea3n \u1ee9ng stress, s\u1ef1 l\u00e3o h\u00f3a l\u00e1 v\u00e0 t\u0103ng tr\u01b0\u1edfng v\u00e0 ph\u00e1t tri\u1ec3n c\u1ee7a c\u00e2y. Nh\u00f3m nghi\u00ean c\u1ee9u \u0111\u00e3 ph\u00e1t hi\u1ec7n ra c\u00e1c m\u1eabu bi\u1ec3u hi\u1ec7n c\u1ee7a m\u1ed9t s\u1ed1 nh\u00f3m IIa GhWRKYc\u00e1c gen trong c\u00e1c m\u00f4 ri\u00eang l\u1ebb c\u0169ng nh\u01b0 trong qu\u00e1 tr\u00ecnh l\u00e3o h\u00f3a l\u00e1 s\u1eed d\u1ee5ng d\u1eef li\u1ec7u gi\u1ea3i tr\u00ecnh t\u1ef1 RNA c\u00f4ng c\u1ed9ng v\u00e0 PCR \u0111\u1ecbnh l\u01b0\u1ee3ng theo th\u1eddi gian th\u1ef1c. \u0110\u1ec3 hi\u1ec3u r\u00f5 h\u01a1n v\u1ec1 c\u00e1c ch\u1ee9c n\u0103ng c\u1ee7a nh\u00f3m IIa GhWRKYs trong b\u00f4ng, GhWRKY17 ( KF669857 ) \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb b\u00f4ng v\u00f9ng cao, s\u1ef1 li\u00ean k\u1ebft chu\u1ed7i, c\u00e1c y\u1ebfu t\u1ed1 ho\u1ea1t h\u00f3a Cis v\u00e0 n\u1ed9i b\u00e0o h\u00f3a \u0111\u01b0\u1ee3c bi\u1ec3u th\u1ecb \u0111\u1eb7c tr\u01b0ng. H\u01a1n n\u1eefa, s\u1ef1 bi\u1ec3u hi\u1ec7n qu\u00e1 m\u1ee9c c\u1ee7a GhWRKY17 trong c\u00e2y Arabidopsis \u0111\u00e3 \u0111i\u1ec1u ch\u1ec9nh c\u00e1c gen li\u00ean quan \u0111\u1ebfn l\u00e3o h\u00f3a AtWRKY53, AtSAG12 v\u00e0 AtSAG13, t\u0103ng c\u01b0\u1eddng t\u00ednh nh\u1ea1y c\u1ea3m c\u1ee7a c\u00e2y \u0111\u1ed1i v\u1edbi s\u1ef1 gi\u00e0 h\u00f3a l\u00e1. Nh\u1eefng ph\u00e1t hi\u1ec7n n\u00e0y \u0111\u1eb7t n\u1ec1n t\u1ea3ng cho ph\u00e2n t\u00edch s\u00e2u h\u01a1n v\u00e0 nghi\u00ean c\u1ee9u c\u00e1c ch\u1ee9c n\u0103ng c\u1ee7a gen WRKY trong b\u00f4ng.<\/span><\/p>\n<p style=\"text-align: right;\"><em><span style=\"font-family: 'times new roman', times, serif; font-size: 12pt;\">Ngu\u1ed3n: ncbi.nlm.nih.gov<\/span><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>B\u00f4ng l\u00e0 m\u1ed9t lo\u1ea1i c\u00e2y tr\u1ed3ng kinh t\u1ebf quan tr\u1ecdng v\u00e0 nguy\u00ean li\u1ec7u d\u1ec7t c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a n\u1ec1n kinh t\u1ebf qu\u1ed1c d\u00e2n. Nh\u1eefng ph\u00e1t hi\u1ec7n tr\u01b0\u1edbc \u0111\u00e2y \u0111\u00e3 ch\u1ec9 ra r\u1eb1ng stress v\u00e0 gi\u00e0 h\u00f3a l\u00e0 nh\u1eefng y\u1ebfu t\u1ed1 quan tr\u1ecdng h\u1ea1n ch\u1ebf s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a b\u00f4ng, [&#8230;]\n","protected":false},"author":1,"featured_media":6477,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6841"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6841"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6841\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6477"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6841"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6841"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6841"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}