{"id":6873,"date":"2019-12-10T08:31:21","date_gmt":"2019-12-10T01:31:21","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=5461"},"modified":"2019-12-10T08:31:21","modified_gmt":"2019-12-10T01:31:21","slug":"nghien-cuu-nghien-cuu-cung-cap-cas9-mot-cong-tac-bat-de-kiem-soat-tot-hon-viec-chinh-sua-gen-crispr","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/nghien-cuu-nghien-cuu-cung-cap-cas9-mot-cong-tac-bat-de-kiem-soat-tot-hon-viec-chinh-sua-gen-crispr\/","title":{"rendered":"Nghi\u00ean c\u1ee9u: Nghi\u00ean c\u1ee9u: Cung c\u1ea5p Cas9 m\u1ed9t c\u00f4ng t\u1eafc ‘b\u1eadt’ \u0111\u1ec3 ki\u1ec3m so\u00e1t t\u1ed1t h\u01a1n vi\u1ec7c ch\u1ec9nh s\u1eeda gen CRISPR"},"content":{"rendered":"

CRISPR-Cas9 l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 mang t\u00ednh c\u00e1ch m\u1ea1ng v\u00e0 m\u1ed9t ph\u1ea7n v\u00ec t\u00ednh linh ho\u1ea1t c\u1ee7a CRISPR-Cas9 v\u00e0 ho\u1ea1t \u0111\u1ed9ng t\u1ed1t trong c\u00e1c t\u1ebf b\u00e0o con ng\u01b0\u1eddi \u0111\u1ec3 th\u1ef1c hi\u1ec7n t\u1ea5t c\u1ea3 c\u00e1c lo\u1ea1i th\u1ee7 thu\u1eadt di truy\u1ec1n, bao g\u1ed3m c\u1eaft v\u00e0 d\u00e1n DNA, t\u1ea1o \u0111\u1ed9t bi\u1ebfn x\u00e1c \u0111\u1ecbnh v\u00e0 k\u00edch ho\u1ea1t ho\u1eb7c b\u1ea5t ho\u1ea1t m\u1ed9t gen.<\/span><\/p>\n

\n

\u0110\u1ea1i h\u1ecdc California, Berkeley v\u00e0 c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u hi\u1ec7n \u0111\u00e3 l\u00e0m cho CRISPR-Cas9 tr\u1edf n\u00ean linh ho\u1ea1t h\u01a1n b\u1eb1ng c\u00e1ch b\u1eadt c\u00f4ng t\u1eafc “b\u1eadt”, cho ph\u00e9p ng\u01b0\u1eddi d\u00f9ng t\u1eaft tr\u00ecnh ch\u1ec9nh s\u1eeda gen Cas9 trong t\u1ea5t c\u1ea3 c\u00e1c t\u1ebf b\u00e0o tr\u1eeb gen m\u1ee5c ti\u00eau \u0111\u01b0\u1ee3c ch\u1ec9 \u0111\u1ecbnh.\u00a0<\/span>Enzim Cas9 \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf l\u1ea1i – c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u g\u1ecdi l\u00e0 ProCas9 c\u00f3 \u0111\u1ea7y \u0111\u1ee7 ch\u1ee9c n\u0103ng ngo\u1ea1i tr\u1eeb vi\u1ec7c ch\u1ee9a m\u1ed9t chi\u1ec1u d\u00e0i protein c\u1ea7n \u0111\u01b0\u1ee3c c\u1eaft tr\u01b0\u1edbc khi enzyme c\u00f3 th\u1ec3 li\u00ean k\u1ebft v\u00e0 c\u1eaft DNA.\u00a0N\u1ebfu c\u00e1c nh\u00e0 khoa h\u1ecdc ch\u00e8n m\u1ed9t \u0111o\u1ea1n protein ng\u1eafn c\u00f3 th\u1ec3 c\u1eaft ch\u1ec9 b\u1eb1ng m\u1ed9t lo\u1ea1i enzyme c\u1ee5 th\u1ec3, ch\u1eb3ng h\u1ea1n nh\u01b0 m\u1ed9t lo\u1ea1i \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ri\u00eang cho t\u1ebf b\u00e0o ung th\u01b0 ho\u1eb7c virus ho\u1eb7c vi khu\u1ea9n truy\u1ec1n nhi\u1ec5m, th\u00ec enzyme \u0111\u00f3 s\u1ebd tr\u1edf th\u00e0nh t\u00e1c nh\u00e2n k\u00edch ho\u1ea1t Cas9.\u00a0<\/span>ProCas9 v\u1ec1 c\u01a1 b\u1ea3n “c\u1ea3m nh\u1eadn” lo\u1ea1i t\u1ebf b\u00e0o d\u1ef1a tr\u00ean enzyme c\u1eaft protein g\u1ecdi l\u00e0 protease.\u00a0<\/span>N\u00f3 c\u0169ng cung c\u1ea5p protein Cas9 v\u1edbi c\u00e1c \u0111\u1ea7u v\u00e0o c\u00f3 th\u1ec3 l\u1eadp tr\u00ecnh c\u00f9ng v\u1edbi c\u00e1c \u0111\u1ea7u ra.<\/span><\/p>\n

“C\u00f3 r\u1ea5t nhi\u1ec1u protease \u0111i\u1ec1u ch\u1ec9nh c\u00e1c con \u0111\u01b0\u1eddng truy\u1ec1n t\u00edn hi\u1ec7u trong c\u00e1c t\u1ebf b\u00e0o, bi\u1ebfn c\u00e1c t\u1ebf b\u00e0o b\u00ecnh th\u01b0\u1eddng th\u00e0nh t\u1ebf b\u00e0o ung th\u01b0 v\u00e0 c\u00f3 li\u00ean quan \u0111\u1ebfn nhi\u1ec5m tr\u00f9ng m\u1ea7m b\u1ec7nh”, Savage n\u00f3i.\u00a0“N\u1ebfu ch\u00fang ta c\u00f3 th\u1ec3 c\u1ea3m nh\u1eadn \u0111\u01b0\u1ee3c c\u00e1c t\u00edn hi\u1ec7u n\u00e0y, ch\u00fang ta c\u00f3 th\u1ec3 ch\u1ea1m v\u00e0o v\u00e0 ph\u1ea3n h\u1ed3i ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c con \u0111\u01b0\u1eddng quan tr\u1ecdng n\u00e0y.”<\/span><\/p>\n

Trong nghi\u00ean c\u1ee9u, Savage v\u00e0 c\u00e1c \u0111\u1ed3ng nghi\u1ec7p \u0111\u00e3 ch\u1ee9ng minh s\u1ef1 ki\u1ec3m so\u00e1t protease c\u1ee7a Cas9 b\u1eb1ng c\u00e1ch l\u00e0m cho n\u00f3 nh\u1ea1y c\u1ea3m v\u1edbi c\u1ea3 virut th\u1ef1c v\u1eadt v\u00e0 ng\u01b0\u1eddi, nh\u01b0 Virus West Nile.\u00a0Trong t\u01b0\u01a1ng lai, h\u1ecd tin r\u1eb1ng lo\u1ea1i c\u00f4ng ngh\u1ec7 n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 nh\u1eadp h\u1ec7 th\u1ed1ng mi\u1ec5n d\u1ecbch vi khu\u1ea9n CRISPR-Cas9 v\u00e0o th\u1ef1c v\u1eadt \u0111\u1ec3 gi\u00fap ch\u00fang ch\u1ed1ng l\u1ea1i c\u00e1c m\u1ea7m b\u1ec7nh g\u00e2y h\u1ea1i.\u00a0<\/span>M\u1ee5c ti\u00eau nghi\u00ean c\u1ee9u ban \u0111\u1ea7u c\u1ee7a Savage l\u00e0 c\u1eaft gi\u1ea3m protein Cas9 nh\u01b0 “chi\u1ebfc k\u00e9o” c\u1eaft DNA \u0111\u1ec3 ch\u1ec9nh s\u1eeda gen \u0111\u1ebfn nh\u1eefng ph\u1ea7n \u0111\u01a1n gi\u1ea3n nh\u1ea5t c\u1ee7a n\u00f3, \u0111\u1ec3 c\u00f3 \u0111\u01b0\u1ee3c tr\u00ecnh ch\u1ec9nh s\u1eeda gen m\u1ea1nh m\u1ebd nh\u1ea5t c\u00f3 th\u1ec3.\u00a0C\u00e0ng \u0111\u01a1n gi\u1ea3n, n\u00f3 c\u00e0ng d\u1ec5 l\u00e0m vi\u1ec7c v\u00e0 chuy\u1ec3n v\u00e0o c\u00e1c t\u1ebf b\u00e0o.\u00a0<\/span>“Ch\u00fang t\u00f4i bi\u1ebft c\u00e1c protein Cas r\u1ea5t ph\u1ee9c t\u1ea1p v\u00e0 protein Cas\u00a0c\u00f3 t\u1ea5t c\u1ea3 c\u00e1c lo\u1ea1i quy \u0111\u1ecbnh r\u1ea5t quan tr\u1ecdng ho\u1ea1t \u0111\u1ed9ng trong h\u1ec7 th\u1ed1ng mi\u1ec5n d\u1ecbch c\u1ee7a vi khu\u1ea9n”.\u00a0“M\u1ee5c ti\u00eau l\u1edbn trong c\u00f4ng vi\u1ec7c c\u1ee7a ch\u00fang t\u00f4i l\u00e0 ch\u1ebf ng\u1ef1 ch\u00fang \u0111\u1ec3 s\u1eed d\u1ee5ng \u1edf ng\u01b0\u1eddi v\u00e0 lo\u1ea1i b\u1ecf nh\u1eefng th\u1ee9 kh\u00f4ng c\u1ea7n thi\u1ebft kh\u00f4ng li\u00ean quan \u0111\u1ebfn ch\u1ec9nh s\u1eeda b\u1ed9 gen.”<\/span><\/p>\n

\u0110\u1eb7c bi\u1ec7t, Savage mu\u1ed1n thi\u1ebft k\u1ebf l\u1ea1i Cas9 \u0111\u1ec3 vi\u1ec7c g\u1eafn c\u00e1c protein kh\u00e1c s\u1ebd d\u1ec5 d\u00e0ng h\u01a1n.\u00a0\u0110i\u1ec1u n\u00e0y s\u1ebd cho ph\u00e9p Cas9 mang protein v\u1edbi nhi\u1ec1u ch\u1ee9c n\u0103ng \u0111\u1ebfn \u0111\u00fang v\u1ecb tr\u00ed tr\u00ean DNA.\u00a0Ch\u00fang \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 protein t\u1ed5ng h\u1ee3p, c\u00e1c bi\u1ebfn th\u1ec3 h\u1ee9a h\u1eb9n c\u00f3 th\u1ec3 thay \u0111\u1ed5i bi\u1ec3u hi\u1ec7n gen ho\u1eb7c trong tr\u01b0\u1eddng h\u1ee3p c\u00f4ng ngh\u1ec7 \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 ch\u1ec9nh s\u1eeda c\u01a1 s\u1edf, thay \u0111\u1ed5i m\u1ed9t c\u01a1 s\u1edf ho\u1eb7c nucleotide trong DNA v\u1edbi \u0111\u1ed9 ch\u00ednh x\u00e1c.\u00a0<\/span>K\u1ef9 thu\u1eadt m\u00e0 \u00f4ng \u0111\u00e3 s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1i c\u1ea5u tr\u00fac Cas9, \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 ho\u00e1n v\u1ecb v\u00f2ng tr\u00f2n, ch\u01b0a bao gi\u1edd \u0111\u01b0\u1ee3c th\u1eed tr\u00ean m\u1ed9t lo\u1ea1i protein ph\u1ee9c t\u1ea1p nh\u01b0 Cas9.\u00a0Ho\u00e1n v\u1ecb v\u00f2ng tr\u00f2n li\u00ean quan \u0111\u1ebfn vi\u1ec7c l\u1ea5y chu\u1ed7i axit amin c\u1ee7a protein Cas9 v\u00e0 c\u1eaft n\u00f3, chuy\u1ec3n \u0111\u1ed5i th\u1ee9 t\u1ef1 c\u1ee7a hai ph\u00e2n \u0111o\u1ea1n v\u00e0 sau \u0111\u00f3 cho ph\u00e9p n\u00f3 g\u1ea5p l\u1ea1i th\u00e0nh c\u1ea5u h\u00ecnh 3D m\u1edbi.\u00a0\u00d4ng \u0111\u00e3 l\u00e0m \u0111i\u1ec1u n\u00e0y cho t\u1ea5t c\u1ea3 c\u00e1c c\u00e1ch c\u00f3 th\u1ec3 c\u1eaft protein th\u00e0nh hai ph\u1ea7n.<\/span><\/p>\n

Savage hi\u1ec7n \u0111ang kh\u00e1m ph\u00e1 m\u1ed9t s\u1ed1 s\u1eafp x\u1ebfp l\u1ea1i Cas9 c\u00f3 th\u1ec3 cung c\u1ea5p t\u1ed1t h\u01a1n cho protein t\u1ed5ng h\u1ee3p, \u0111\u01b0a ch\u00fang \u0111\u1ebfn g\u1ea7n h\u01a1n v\u1edbi chu\u1ed7i DNA m\u1ee5c ti\u00eau.\u00a0<\/span>Trong qu\u00e1 tr\u00ecnh s\u1eafp x\u1ebfp l\u1ea1i\u00a0Cas9, anh th\u1ea5y r\u1eb1ng c\u00e1ch k\u1ebft n\u1ed1i l\u1ea1i hai ph\u00e2n \u0111o\u1ea1n protein \u0111\u00e3 t\u1ea1o ra s\u1ef1 kh\u00e1c bi\u1ec7t.\u00a0<\/span>“Khi ch\u00fang t\u00f4i c\u1eaft protein v\u00e0 chuy\u1ec3n m\u1ea3nh c\u0169 \u0111\u1ebfn m\u1ed9t v\u1ecb tr\u00ed m\u1edbi trong protein, h\u1ec7 th\u1ed1ng tr\u1edf n\u00ean r\u1ea5t nh\u1ea1y c\u1ea3m v\u1edbi c\u00e1ch b\u1ea1n li\u00ean k\u1ebft hai m\u1ea3nh l\u1ea1i v\u1edbi nhau”, \u00f4ng n\u00f3i.\u00a0“Ch\u00fang t\u00f4i nh\u1eadn ra r\u1eb1ng ch\u00fang t\u00f4i c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng \u0111\u1ed9 nh\u1ea1y \u0111\u00f3 \u0111\u1ec3 thi\u1ebft k\u1ebf protein \u0111\u1ec3 c\u00f3 c\u00e1c v\u1ecb tr\u00ed nh\u1eadn bi\u1ebft protease.”<\/span><\/p>\n

Ngu\u1ed3n: sciencedaily.com<\/span><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

CRISPR-Cas9 l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 mang t\u00ednh c\u00e1ch m\u1ea1ng v\u00e0 m\u1ed9t ph\u1ea7n v\u00ec t\u00ednh linh ho\u1ea1t c\u1ee7a CRISPR-Cas9 v\u00e0 ho\u1ea1t \u0111\u1ed9ng t\u1ed1t trong c\u00e1c t\u1ebf b\u00e0o con ng\u01b0\u1eddi \u0111\u1ec3 th\u1ef1c hi\u1ec7n t\u1ea5t c\u1ea3 c\u00e1c lo\u1ea1i th\u1ee7 thu\u1eadt di truy\u1ec1n, bao g\u1ed3m c\u1eaft v\u00e0 d\u00e1n DNA, t\u1ea1o \u0111\u1ed9t bi\u1ebfn x\u00e1c \u0111\u1ecbnh v\u00e0 k\u00edch ho\u1ea1t ho\u1eb7c b\u1ea5t […]\n","protected":false},"author":1,"featured_media":6894,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6873"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6873"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6873\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6894"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6873"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6873"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6873"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}