{"id":6878,"date":"2019-12-13T15:10:45","date_gmt":"2019-12-13T08:10:45","guid":{"rendered":"http:\/\/antoansinhhoc.vn\/?p=5601"},"modified":"2019-12-13T15:10:45","modified_gmt":"2019-12-13T08:10:45","slug":"nghien-cuu-trinh-tu-the-he-tiep-theo-duoc-co-muc-tieu-de-nghien-cuu-tinh-on-dinh-cua-cay-trong-trong-cay-bien-doi-gen","status":"publish","type":"post","link":"https:\/\/nbca.gov.vn\/nghien-cuu-trinh-tu-the-he-tiep-theo-duoc-co-muc-tieu-de-nghien-cuu-tinh-on-dinh-cua-cay-trong-trong-cay-bien-doi-gen\/","title":{"rendered":"Nghi\u00ean c\u1ee9u: Tr\u00ecnh t\u1ef1 th\u1ebf h\u1ec7 ti\u1ebfp theo \u0111\u01b0\u1ee3c c\u00f3 m\u1ee5c ti\u00eau \u0111\u1ec3 nghi\u00ean c\u1ee9u t\u00ednh \u1ed5n \u0111\u1ecbnh c\u1ee7a c\u00e2y tr\u1ed3ng trong c\u00e2y bi\u1ebfn \u0111\u1ed5i gen"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif;\">Ch\u1ec9 th\u1ecb c\u1ee7a EU 2001\/18\/EC y\u00eau c\u1ea7u b\u1ea5t k\u1ef3 s\u1ef1 ki\u1ec7n bi\u1ebfn \u0111\u1ed5i gen (GM) n\u00e0o ph\u1ea3i \u1ed5n \u0111\u1ecbnh. Trong c\u00f4ng vi\u1ec7c hi\u1ec7n t\u1ea1i, m\u1ed9t c\u00e1ch ti\u1ebfp c\u1eadn C\u00f4ng ngh\u1ec7 gi\u1ea3i tr\u00ecnh t\u1ef1 th\u1ebf h\u1ec7 m\u1edbi (NGS) \u0111\u01b0\u1ee3c nh\u1eafm m\u1ee5c ti\u00eau s\u1eed d\u1ee5ng m\u00e3 v\u1ea1ch \u0111\u1ec3 g\u1eafn th\u1ebb c\u1ee5 th\u1ec3 t\u1eebng ph\u00e2n t\u1eed DNA ri\u00eang l\u1ebb trong qu\u00e1 tr\u00ecnh chu\u1ea9n b\u1ecb th\u01b0 vi\u1ec7n \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c \u0111\u1ed9t bi\u1ebfn c\u00f3 t\u00ednh \u0111\u1ebfn nhi\u1ec5u n\u1ec1n do l\u1ed7i khu\u1ebfch \u0111\u1ea1i v\u00e0 gi\u1ea3i tr\u00ecnh t\u1ef1. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y l\u1ea7n \u0111\u1ea7u ti\u00ean \u0111\u01b0\u1ee3c ch\u1ee9ng minh l\u00e0 c\u00f3 hi\u1ec7u qu\u1ea3 trong vi\u1ec7c ph\u00e1t hi\u1ec7n c\u00e1c \u0111\u1ed9t bi\u1ebfn trong c\u00e1c m\u1eabu t\u1ed5ng h\u1ee3p \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb v\u1edbi c\u00e1c tr\u00ecnh t\u1ef1 P35S \u0111\u1ed9t bi\u1ebfn t\u1ed5ng h\u1ee3p ho\u1eb7c kh\u00f4ng \u0111\u1ed9t bi\u1ebfn \u0111\u01b0\u1ee3c tr\u1ed9n l\u1eabn theo c\u00e1c t\u1ef7 l\u1ec7 kh\u00e1c nhau. T\u00ednh \u1ed5n \u0111\u1ecbnh di truy\u1ec1n c\u1ee7a m\u1ed9t ph\u1ea7n c\u1ee7a ch\u1ea5t k\u00edch th\u00edch P35S \u0111\u01b0\u1ee3c nh\u1eafm m\u1ee5c ti\u00eau ph\u00e1t hi\u1ec7n GM sau \u0111\u00f3 \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch trong c\u00e1c m\u1eabu b\u1ed9t GM.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif;\">M\u1ed9t s\u1ed1 \u0111\u1ed9t bi\u1ebfn t\u1ea7n s\u1ed1 th\u1ea5p \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n trong tr\u00ecnh t\u1ef1 P35S. M\u1ed9t s\u1ed1 nucleotide b\u1ecb \u0111\u1ed9t bi\u1ebfn n\u1eb1m trong \u0111o\u1ea1n m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n P35S. N\u1ebfu hi\u1ec7n t\u1ea1i kh\u00f4ng ph\u1ea3i l\u00e0 \u0111\u1ed9t bi\u1ebfn soma m\u00e0 l\u00e0 tr\u00ecnh t\u1ef1 t\u01b0\u01a1ng \u0111\u1ed3ng c\u1ee7a m\u1ed9t s\u1ed1 \u0111\u01a1n v\u1ecb, nh\u1eefng \u0111\u1ed9t bi\u1ebfn n\u00e0y c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u qu\u1ea3 c\u1ee7a x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n PCR th\u1eddi gian th\u1ef1c P35S. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong c\u00e1c nghi\u00ean c\u1ee9u v\u1ec1 t\u00ednh \u1ed5n \u0111\u1ecbnh di truy\u1ec1n c\u1ee7a c\u00e1c ch\u00e8n GM nh\u01b0ng c\u0169ng \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c c\u00e2y bi\u1ebfn \u0111\u1ed5i gen nucleotide \u0111\u01a1n \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c k\u1ef9 thu\u1eadt nh\u00e2n gi\u1ed1ng m\u1edbi.<\/span><\/p>\n<p style=\"text-align: right;\"><span style=\"font-family: arial, helvetica, sans-serif;\">Ngu\u1ed3n: ncbi.nlm.nih.gov<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ch\u1ec9 th\u1ecb c\u1ee7a EU 2001\/18\/EC y\u00eau c\u1ea7u b\u1ea5t k\u1ef3 s\u1ef1 ki\u1ec7n bi\u1ebfn \u0111\u1ed5i gen (GM) n\u00e0o ph\u1ea3i \u1ed5n \u0111\u1ecbnh. Trong c\u00f4ng vi\u1ec7c hi\u1ec7n t\u1ea1i, m\u1ed9t c\u00e1ch ti\u1ebfp c\u1eadn C\u00f4ng ngh\u1ec7 gi\u1ea3i tr\u00ecnh t\u1ef1 th\u1ebf h\u1ec7 m\u1edbi (NGS) \u0111\u01b0\u1ee3c nh\u1eafm m\u1ee5c ti\u00eau s\u1eed d\u1ee5ng m\u00e3 v\u1ea1ch \u0111\u1ec3 g\u1eafn th\u1ebb c\u1ee5 th\u1ec3 t\u1eebng ph\u00e2n t\u1eed DNA ri\u00eang [&#8230;]\n","protected":false},"author":1,"featured_media":6894,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6878"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=6878"}],"version-history":[{"count":0,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/6878\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6894"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=6878"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=6878"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=6878"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}