Nh\u1eefng n\u0103m tr\u1edf l\u1ea1i \u0111\u00e2y, c\u00e1c nh\u00e0 khoa h\u1ecdc Vi\u1ec7t Nam \u0111\u00e3 c\u00f4ng b\u1ed1 nhi\u1ec1u c\u00f4ng tr\u00ecnh li\u00ean quan \u0111\u1ebfn c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gene tr\u00ean n\u00f4ng s\u1ea3n, t\u1eeb c\u00e1c k\u1ebft qu\u1ea3 \u0111\u1ec1 t\u00e0i khoa h\u1ecdc c\u1ea5p Th\u00e0nh ph\u1ed1, c\u1ea5p Qu\u1ed1c gia, \u0111\u1ebfn c\u00e1c b\u00e0i b\u00e1o khoa h\u1ecdc \u0111\u0103ng t\u1ea3i tr\u00ean c\u00e1c t\u1ea1p ch\u00ed uy t\u00edn th\u1ebf gi\u1edbi.<\/p>\n
\u0110\u1ed1i v\u1edbi c\u00e2y l\u00faa<\/strong><\/p>\n V\u1edbi c\u00e2y l\u00faa, c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gene \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00e1c nh\u00e0 khoa h\u1ecdc tri\u1ec3n khai nghi\u00ean c\u1ee9u v\u00e0 \u1ee9ng d\u1ee5ng trong c\u00e1c \u0111\u1ec1 t\u00e0i khoa h\u1ecdc c\u1ea5p Qu\u1ed1c gia, nghi\u1ec7m thu k\u1ebft qu\u1ea3 n\u0103m 2021: \u201cNghi\u00ean c\u1ee9u \u1ee9ng d\u1ee5ng c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda h\u1ec7 gene \u0111\u1ec3 c\u1ea3i t\u1ea1o t\u00ednh tr\u1ea1ng m\u00f9i th\u01a1m v\u00e0 kh\u00e1ng b\u1ea1c l\u00e1 tr\u00ean m\u1ed9t s\u1ed1 gi\u1ed1ng l\u00faa ch\u1ee7 l\u1ef1c c\u1ee7a Vi\u1ec7t Nam\u201d do Vi\u1ec7n Di truy\u1ec1n N\u00f4ng nghi\u1ec7p (Vi\u1ec7n Khoa h\u1ecdc N\u00f4ng nghi\u1ec7p Vi\u1ec7t Nam – B\u1ed9 N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n) ch\u1ee7 tr\u00ec th\u1ef1c hi\u1ec7n.<\/p>\n K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u \u0111\u00e3 x\u00e2y d\u1ef1ng quy tr\u00ecnh, t\u1ea1o m\u1ed9t s\u1ed1 d\u00f2ng l\u00faa B\u1eafc th\u01a1m 7. Trong \u0111i\u1ec1u ki\u1ec7n nh\u00e0 l\u01b0\u1edbi, t\u1ea5t c\u1ea3 d\u00f2ng l\u00faa BT7 ch\u1ec9nh s\u1eeda gene \u0111\u1ec1u kh\u00f4ng c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 ch\u1ec9 ti\u00eau n\u00f4ng h\u1ecdc so v\u1edbi d\u00f2ng l\u00faa \u0111\u1ed1i ch\u1ee9ng kh\u00f4ng ch\u1ec9nh s\u1eeda gene. Ba d\u00f2ng l\u00faa BT7 (1.12.07, 1.15.21 v\u00e0 3.01.19) kh\u00f4ng thay \u0111\u1ed5i m\u1ee9c \u0111\u1ed9 bi\u1ec3u hi\u1ec7n OsSWEET14 khi \u0111\u01b0\u1ee3c l\u00e2y nhi\u1ec5m nh\u00e2n t\u1ea1o v\u1edbi 3 isolate Xoo (vi khu\u1ea9n g\u00e2y b\u1ec7nh b\u1ea1c l\u00e1 tr\u00ean l\u00faa – Xanthomonas oryzae pv. oryzae – Xoo). C\u1ea3 3 d\u00f2ng l\u00faa \u0111\u1ec1u th\u1ec3 hi\u1ec7n t\u00ednh kh\u00e1ng r\u00f5 r\u1ec7t v\u1edbi VXO_ll v\u00e0 kh\u00e1ng nh\u1eb9 v\u1edbi VXO_96 cho th\u1ea5y tri\u1ec3n v\u1ecdng c\u1ea3i ti\u1ebfn t\u00ednh kh\u00e1ng b\u1ea1c l\u00e1 cho c\u00e1c gi\u1ed1ng l\u00faa \u01b0u t\u00fa nh\u01b0 BT7 th\u00f4ng qua \u0111\u1ed9t bi\u1ebfn gene \u0111\u00edch b\u1eb1ng c\u00f4ng ngh\u1ec7 CRISPR\/Cas9. B\u00ean c\u1ea1nh \u0111\u00f3, k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u c\u0169ng t\u1ea1o ra d\u00f2ng l\u00faa OM5451 \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ec9nh s\u1eeda gene BADH2 c\u00f3 m\u00f9i th\u01a1m t\u01b0\u01a1ng \u0111\u01b0\u01a1ng gi\u1ed1ng OM6162 m\u00e0 kh\u00f4ng b\u1ecb thay \u0111\u1ed5i ch\u1ea5t l\u01b0\u1ee3ng.<\/p>\n Tr\u00ean c\u00e2y d\u01b0a leo<\/strong><\/p>\n Nh\u1eb1m b\u01b0\u1edbc \u0111\u1ea7u nghi\u00ean c\u1ee9u th\u1eed nghi\u1ec7m t\u1ea1o gi\u1ed1ng d\u01b0a leo c\u00f3 kh\u1ea3 n\u0103ng kh\u00e1ng virus g\u00e2y b\u1ec7nh v\u00e0ng g\u00e2n d\u01b0a leo CVYV (Cucumber vein yellowing virus) v\u00e0 virus g\u00e2y b\u1ec7nh kh\u1ea3m l\u00e1 d\u01b0a leo CMV (Cucumber mosaic virus), t\u1eeb n\u0103m 2018, c\u00e1c nh\u00e0 khoa h\u1ecdc t\u1ea1i Trung t\u00e2m C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc TP.HCM \u0111\u00e3 ti\u1ebfn h\u00e0nh th\u1ef1c hi\u1ec7n \u0111\u1ec1 t\u00e0i \u201cNghi\u00ean c\u1ee9u \u1ee9ng d\u1ee5ng c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gene CRISPR\/Cas9 tr\u00ean c\u00e2y d\u01b0a leo h\u01b0\u1edbng \u0111\u1ebfn t\u1ea1o gi\u1ed1ng d\u01b0a leo c\u00f3 kh\u1ea3 n\u0103ng kh\u00e1ng virus\u201d, k\u1ebft qu\u1ea3 v\u1eeba \u0111\u01b0\u1ee3c S\u1edf Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 TP.HCM nghi\u1ec7m thu trong n\u0103m 2022.<\/p>\n Ph\u00e2n t\u00edch cho th\u1ea5y h\u1ea7u h\u1ebft c\u00e1c m\u1eabu chuy\u1ec3n gene \u0111\u1ec1u b\u1ecb nhi\u1ec5m virus v\u00e0 c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 m\u1ee9c \u0111\u1ed9 l\u00e2y nhi\u1ec5m gi\u1eefa c\u00e1c m\u1eabu chuy\u1ec3n gene nh\u01b0ng ch\u01b0a cho th\u1ea5y t\u00ednh kh\u00e1ng virus bi\u1ec3u hi\u1ec7n r\u00f5 r\u00e0ng \u1edf t\u1ea5t c\u1ea3 c\u00e1c m\u1eabu. M\u1eb7c d\u00f9 c\u1ea7n nh\u1eefng nghi\u00ean c\u1ee9u \u0111\u00e1nh gi\u00e1 s\u00e2u h\u01a1n v\u1ec1 kh\u1ea3 n\u0103ng kh\u00e1ng virus, nh\u01b0ng \u0111\u00ea\u0300 ta\u0300i c\u1ee7a c\u00e1c nh\u00e0 khoa h\u1ecdc t\u1ea1i Trung t\u00e2m C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc TP.HCM \u0111\u00e3 g\u00f3p ph\u1ea7n t\u1ea1o ti\u1ec1n \u0111\u1ec1 \u1ee9ng d\u1ee5ng c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gen CRISPR\/Cas9 v\u00e0o nghi\u00ean c\u1ee9u t\u1ea1o gi\u1ed1ng c\u00e2y tr\u1ed3ng t\u1ea1i Vi\u1ec7t Nam, \u0111\u1eb7c bi\u1ec7t l\u00e0 t\u1ea1o gi\u1ed1ng kh\u00e1ng virus cho n\u00f4ng s\u1ea3n.<\/p>\n \u00a0\u0110u \u0111\u1ee7<\/strong><\/p>\n Ngay t\u1eeb n\u0103m 2016, c\u00e1c nh\u00e0 khoa h\u1ecdc t\u1ea1i Vi\u1ec7n C\u00f4ng ngh\u1ec7 sinh h\u1ecdc (Vi\u1ec7n H\u00e0n l\u00e2m KH&CN Vi\u1ec7t Nam) \u0111\u00e3 nghi\u00ean c\u1ee9u v\u00e0 \u00e1p d\u1ee5ng c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gene tr\u00ean c\u00e2y \u0111u \u0111\u1ee7 th\u00f4ng qua h\u1ec7 th\u1ed1ng CRISPR\/Cas9 nh\u1eb1m t\u00ecm ra ph\u01b0\u01a1ng ph\u00e1p x\u1eed l\u00fd b\u1ec7nh \u0111\u1ed1m v\u00f2ng do virus PRSV (Papaya ringspot virus) g\u00e2y ra.<\/p>\n K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u \u0111\u00e3 t\u1ea1o \u0111\u01b0\u1ee3c c\u00e2y \u0111u \u0111\u1ee7 \u0111\u1ed9t bi\u1ebfn, \u0111\u1ecbnh h\u01b0\u1edbng tr\u00ean gene eIF4E. \u0110\u00e1nh gi\u00e1 b\u01b0\u1edbc \u0111\u1ea7u trong \u0111i\u1ec1u ki\u1ec7n nh\u00e0 l\u01b0\u1edbi cho th\u1ea5y, c\u00e1c d\u00f2ng \u0111u \u0111\u1ee7 ch\u1ec9nh s\u1eeda gene c\u00f3 t\u00ednh kh\u00e1ng ho\u00e0n to\u00e0n v\u1edbi b\u1ec7nh virus \u0111\u1ed1m v\u00f2ng. Ngo\u00e0i ra, \u1edf c\u00e1c d\u00f2ng c\u00e2y \u0111u \u0111\u1ee7 ch\u1ec9nh s\u1eeda gene, kh\u00f4ng ghi nh\u1eadn s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 h\u00ecnh th\u00e1i, sinh tr\u01b0\u1edfng ph\u00e1t tri\u1ec3n v\u00e0 kh\u1ea3 n\u0103ng t\u1ea1o qu\u1ea3 so v\u1edbi gi\u1ed1ng ch\u01b0a ch\u1ec9nh s\u1eeda gene.<\/p>\n K\u1ebft qu\u1ea3 b\u01b0\u1edbc \u0111\u1ea7u c\u1ee7a nh\u00f3m nghi\u00ean c\u1ee9u v\u1ec1 ch\u1ec9nh s\u1eeda gene tr\u00ean c\u00e2y \u0111u \u0111\u1ee7 \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00f4ng b\u1ed1 tr\u00ean T\u1ea1p ch\u00ed Plant Cell Tissue and Organ Cultures c\u1ee7a NXB Springer Nature v\u00e0o th\u00e1ng 11\/2022, v\u1edbi t\u00ean b\u00e0i b\u00e1o: \u201cDeveloping a robust in vivo hairy root system for assessing transgene expression and genome editing efficiency in papaya\u201d.<\/p>\n T\u0103ng ch\u1ea5t l\u01b0\u1ee3ng qu\u1ea3 c\u00e0 chua<\/strong><\/p>\n Ngo\u00e0i kh\u00e1ng virus g\u00e2y b\u1ec7nh, vi\u1ec7c ch\u1ec9nh s\u1eeda gene c\u0169ng nh\u1eafm \u0111\u1ebfn m\u1ee5c ti\u00eau t\u0103ng ch\u1ea5t l\u01b0\u1ee3ng n\u00f4ng s\u1ea3n, nghi\u00ean c\u1ee9u \u201c\u1ee8ng d\u1ee5ng c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gene \u0111\u1ec3 l\u00e0m t\u0103ng h\u00e0m l\u01b0\u1ee3ng \u0111\u01b0\u1eddng v\u00e0 c\u00e1c axit amin c\u00f3 l\u1ee3i cho s\u1ee9c kh\u1ecfe tr\u00ean qu\u1ea3 c\u00e0 chua\u201d \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00e1c nh\u00e0 khoa h\u1ecdc c\u1ee7a Vi\u1ec7n C\u00f4ng ngh\u1ec7 sinh h\u1ecdc (Vi\u1ec7n H\u00e0n l\u00e2m KH&CN Vi\u1ec7t Nam) th\u1ef1c hi\u1ec7n tr\u00ean gi\u1ed1ng c\u00e0 chua PT18 c\u1ee7a Vi\u1ec7t Nam.<\/p>\n K\u1ebft qu\u1ea3 ph\u00e2n t\u00edch th\u00e0nh ph\u1ea7n qu\u1ea3 c\u1ee7a c\u00e1c d\u00f2ng c\u00e2y \u0111\u01b0\u1ee3c ch\u1ec9nh s\u1eeda gene cho th\u1ea5y, c\u00f3 s\u1ef1 gia t\u0103ng \u0111\u00e1ng k\u1ec3 h\u00e0m l\u01b0\u1ee3ng \u0111\u01b0\u1eddng v\u00e0 axit amin t\u1ed5ng.<\/p>\n Chia s\u1ebb, h\u1ecdc t\u1eadp kinh nghi\u1ec7m c\u1ee7a c\u00e1c qu\u1ed1c gia \u0111i tr\u01b0\u1edbc v\u00e0 trao \u0111\u1ed5i, ph\u1ed5 bi\u1ebfn th\u00f4ng tin v\u1ec1 th\u00e0nh t\u1ef1u c\u1ee7a c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gen, ti\u1ec1m n\u0103ng, l\u1ee3i \u00edch c\u1ee7a c\u00f4ng ngh\u1ec7 l\u00e0 vi\u1ec7c l\u00e0m c\u1ea7n thi\u1ebft, li\u00ean t\u1ee5c \u0111\u1ec3 khai th\u00e1c \u1ee9ng d\u1ee5ng c\u00f3 hi\u1ec7u qu\u1ea3 c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gen trong \u0111i\u1ec1u ki\u1ec7n Vi\u1ec7t Nam ph\u00e1t tri\u1ec3n n\u00f4ng nghi\u1ec7p b\u1ec1n v\u1eefng.\/.<\/p>\n NBCA<\/strong><\/p>\n \n","protected":false},"excerpt":{"rendered":" Nh\u1eefng n\u0103m tr\u1edf l\u1ea1i \u0111\u00e2y, c\u00e1c nh\u00e0 khoa h\u1ecdc Vi\u1ec7t Nam \u0111\u00e3 c\u00f4ng b\u1ed1 nhi\u1ec1u c\u00f4ng tr\u00ecnh li\u00ean quan \u0111\u1ebfn c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gene tr\u00ean n\u00f4ng s\u1ea3n, t\u1eeb c\u00e1c k\u1ebft qu\u1ea3 \u0111\u1ec1 t\u00e0i khoa h\u1ecdc c\u1ea5p Th\u00e0nh ph\u1ed1, c\u1ea5p Qu\u1ed1c gia, \u0111\u1ebfn c\u00e1c b\u00e0i b\u00e1o khoa h\u1ecdc \u0111\u0103ng t\u1ea3i tr\u00ean c\u00e1c t\u1ea1p ch\u00ed uy […]\n","protected":false},"author":10,"featured_media":6894,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/9232"}],"collection":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/comments?post=9232"}],"version-history":[{"count":1,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/9232\/revisions"}],"predecessor-version":[{"id":9233,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/posts\/9232\/revisions\/9233"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media\/6894"}],"wp:attachment":[{"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/media?parent=9232"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/categories?post=9232"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbca.gov.vn\/wp-json\/wp\/v2\/tags?post=9232"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}